C57BL/6JCya-Card11em1flox/Cya
Common Name:
Card11-flox
Product ID:
S-CKO-18652
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Card11-flox
Strain ID
CKOCMP-108723-Card11-B6J-VB
Gene Name
Product ID
S-CKO-18652
Gene Alias
0610008L17Rik; 2410011D02Rik; BIMP3; CARMA1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Card11em1flox/Cya mice (Catalog S-CKO-18652) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000085786
NCBI RefSeq
NM_175362
Target Region
Exon 4~5
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
CARD11, a lymphoid lineage-specific scaffold protein, is crucial for integrating antigen-receptor engagement with downstream signaling to NF-κB and other outputs in lymphocytes [2,4]. It forms the CARD11-BCL10-MALT1 (CBM) signaling complex, which is key in T-and B-cell receptor-induced gene expression by regulating NF-κB activation and mRNA stability [4]. Deregulated CARD11 expression or CBM signaling is associated with immunodeficiency, autoimmunity, and cancer [4]. Mouse models are valuable in studying CARD11 function.
Mouse models with CARD11 and related signaling component deficiencies generally have Treg defects [1]. CARD11 is essential for the development and function of thymic regulatory T cells (Tregs) [1]. Some CARD11-deficient mouse models develop overt autoimmunity and inflammatory disease, while others do not [1]. Also, a germline N-ethyl-N-nitrosourea (ENU)-induced Card11M365K mutation in mice, similar to those in diffuse large B-cell lymphoma (DLBCL) and peripheral T-cell lymphomas (PTCL), increased B and T lymphocyte activation and proliferation following antigen receptor stimulation, and caused over-accumulation of activated T cells, Tregs, T follicular (TFH), and T follicular regulatory (TFR) cells [3].
In conclusion, CARD11 is a key regulator in lymphocyte-mediated immune responses. Its function is essential for Treg development and function, as revealed by mouse models. Dysregulation of CARD11 can lead to various immune-related diseases such as autoimmunity, inflammatory diseases, and lymphoproliferative disorders, highlighting the importance of understanding CARD11 for developing therapies in these disease areas.
References：
1. Carter, Nicole M, Pomerantz, Joel L. 2022. CARD11 signaling in regulatory T cell development and function. In Advances in biological regulation, 84, 100890. doi:10.1016/j.jbior.2022.100890. https://pubmed.ncbi.nlm.nih.gov/35255409/
2. Pomerantz, Joel L, Milner, Joshua D, Snow, Andrew L. 2022. Elevated IgE from attenuated CARD11 signaling: lessons from atopic mice and humans. In Current opinion in immunology, 79, 102255. doi:10.1016/j.coi.2022.102255. https://pubmed.ncbi.nlm.nih.gov/36334349/
3. Masle-Farquhar, Etienne, Jeelall, Yogesh, White, Jacqueline, Horikawa, Keisuke, Goodnow, Christopher Carl. 2023. CARD11 gain-of-function mutation drives cell-autonomous accumulation of PD-1+ ICOShigh activated T cells, T-follicular, T-regulatory and T-follicular regulatory cells. In Frontiers in immunology, 14, 1095257. doi:10.3389/fimmu.2023.1095257. https://pubmed.ncbi.nlm.nih.gov/36960072/
4. Lork, Marie, Staal, Jens, Beyaert, Rudi. 2018. Ubiquitination and phosphorylation of the CARD11-BCL10-MALT1 signalosome in T cells. In Cellular immunology, 340, 103877. doi:10.1016/j.cellimm.2018.11.001. https://pubmed.ncbi.nlm.nih.gov/30514565/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen