C57BL/6JCya-Lipgem1flox/Cya
Common Name:
Lipg-flox
Product ID:
S-CKO-18705
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Lipg-flox
Strain ID
CKOCMP-16891-Lipg-B6J-VB
Gene Name
Product ID
S-CKO-18705
Gene Alias
3110013K01Rik; EL; lipase; mEDL
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lipgem1flox/Cya mice (Catalog S-CKO-18705) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000066532
NCBI RefSeq
NM_010720
Target Region
Exon 2~3
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Lipg, also known as endothelial lipase (EL), is a gene with crucial functions in the human body. It predominantly acts as a phospholipase and also exhibits low levels of triglyceride lipase activity. It plays an essential role in lipoprotein metabolism and is involved in metabolic syndromes such as the inflammatory response and atherosclerosis [1].
In breast cancer, studies have shown that the risk of breast cancer increases with higher levels of LIPG, especially in pre-menopausal, Luminal A, HER2-negative, non-postpartum, and grades II and III breast cancers [2]. LIPG-promoted lipid storage in breast cancer cells mediates adaptation to oxidative stress, which supports tumor progression [3].
In lung adenocarcinoma, LIPG expression is higher in tumor tissues, especially in certain subgroups, and is associated with a poor prognosis, lipid metabolism, and immune infiltration [4].
In colorectal cancer, ZDHHC1 downregulates LIPG to inhibit cancer cell growth [5].
In human basal-like triple-negative breast cancer, LIPG is overexpressed and is required for tumorigenicity and metastasis, and its phospholipase activity is related to tumor formation [6,7].
Additionally, in the Maonan and Han nationalities in China, certain LIPG SNPs and their haplotypes are associated with serum lipid levels [8].
In conclusion, Lipg is involved in various biological processes, especially in lipid metabolism. Its abnormal expression is closely related to multiple cancers, including breast, lung, and colorectal cancers. The study of Lipg using gene-knockout or conditional-knockout mouse models (although not explicitly mentioned in all references but inferred as a valuable approach) can help further understand its role in these diseases, providing potential directions for targeted therapies.
References:
1. Hong, Chang, Deng, Ruxia, Wang, Ping, Cai, Rui, Lin, Jie. 2020. LIPG: an inflammation and cancer modulator. In Cancer gene therapy, 28, 27-32. doi:10.1038/s41417-020-0188-5. https://pubmed.ncbi.nlm.nih.gov/32572177/
2. Gago-Dominguez, Manuela, Redondo, Carmen M, Calaza, Manuel, Carracedo, Ángel, Castelao, J Esteban. 2021. LIPG endothelial lipase and breast cancer risk by subtypes. In Scientific reports, 11, 10436. doi:10.1038/s41598-021-89669-4. https://pubmed.ncbi.nlm.nih.gov/34001944/
3. Cadenas, Cristina, Vosbeck, Sonja, Edlund, Karolina, Marchan, Rosemarie, Hengstler, Jan G. 2019. LIPG-promoted lipid storage mediates adaptation to oxidative stress in breast cancer. In International journal of cancer, 145, 901-915. doi:10.1002/ijc.32138. https://pubmed.ncbi.nlm.nih.gov/30653260/
4. Wang, Shan, Chen, Zhaoxin, Lv, Hongwei, Wei, Huamin, Yu, Jing. 2022. LIPG is a novel prognostic biomarker and correlated with immune infiltrates in lung adenocarcinoma. In Journal of clinical laboratory analysis, 37, e24824. doi:10.1002/jcla.24824. https://pubmed.ncbi.nlm.nih.gov/36572999/
5. Zhang, Qun, Du, Zhouyuan, Zhou, Wei, Yu, Haixin, Liu, Tao. 2024. ZDHHC1 downregulates LIPG and inhibits colorectal cancer growth via IGF2BP1 Palmitoylation. In Cancer gene therapy, 31, 1427-1437. doi:10.1038/s41417-024-00808-1. https://pubmed.ncbi.nlm.nih.gov/39069526/
6. Lo, Pang-Kuo, Yao, Yuan, Lee, Ji Shin, Kane, Maureen A, Zhou, Qun. 2018. LIPG signaling promotes tumor initiation and metastasis of human basal-like triple-negative breast cancer. In eLife, 7, . doi:10.7554/eLife.31334. https://pubmed.ncbi.nlm.nih.gov/29350614/
7. Lo, Pang-Kuo, Yao, Yuan, Zhou, Qun. 2020. Inhibition of LIPG phospholipase activity suppresses tumor formation of human basal-like triple-negative breast cancer. In Scientific reports, 10, 8911. doi:10.1038/s41598-020-65400-7. https://pubmed.ncbi.nlm.nih.gov/32488004/
8. Yang, Shuo, Yin, Rui-Xing, Miao, Liu, Wu, Jie, Zhang, Qing-Hui. 2019. LIPG SNPs, their haplotypes and gene-environment interactions on serum lipid levels. In Lipids in health and disease, 18, 10. doi:10.1186/s12944-018-0942-y. https://pubmed.ncbi.nlm.nih.gov/30621702/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen