Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Diaph2em1flox/Cya
Common Name:
Diaph2-flox
Product ID:
S-CKO-18739
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Diaph2-flox
Strain ID
CKOCMP-54004-Diaph2-B6J-VB
Gene Name
Diaph2
Product ID
S-CKO-18739
Gene Alias
Dia3; Diap2; Drf2; E430022I22Rik
Background
C57BL/6JCya
NCBI ID
54004
Modification
Conditional knockout
Chromosome
X
Phenotype
MGI:1858500
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Diaph2em1flox/Cya mice (Catalog S-CKO-18739) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000037854
NCBI RefSeq
NM_172493
Target Region
Exon 6~7
Size of Effective Region
~0.9 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Diaph2, a diaphanous related formin gene, encodes a regulatory cytoskeletal protein involved in actin elongation and microtubule stabilization [5,6,7,8]. It belongs to the formin family and is crucial for processes like cell movement, adhesion, and meiosis/DNA repair pathways [2,4,6]. Genetic models, such as mouse models, can be valuable for studying its function.

Studies have shown that DIAPH2 gene polymorphisms are associated with an increased risk of laryngeal cancer. The rs6620138 polymorphism can raise the onset risk, and there are significant differences in allele distribution in case and control groups [1]. In men, the rs12851931 polymorphism in DIAPH2 may increase the risk of laryngeal squamous cell carcinoma (LSCC), with homozygotes more prevalent in smokers, and the polymorphism is related to the T stage of the tumor [3]. Additionally, DIAPH2 alterations are present primarily in metastasizing specimens of LSCC, and may contribute to the tumor's metastatic potential, as Nuclease technology-edited heterozygous DIAPH2+/- mutant HEK-293T cells show a shift from 'proliferation' to'migration' phenotype [4].

In conclusion, Diaph2 plays important roles in cell movement, adhesion, and may be involved in cancer-related processes such as the development and metastasis of laryngeal cancer. The study of Diaph2 using genetic models has provided insights into its role in these disease conditions, helping to better understand the underlying mechanisms and potentially providing new directions for cancer research and treatment [1,3,4].

References:
1. Śnit, Mirosław, Misiołek, Maciej, Ścierski, Wojciech, Okła, Sławomir, Grzeszczak, Władysław. 2021. DIAPH2, PTPRD and HIC1 Gene Polymorphisms and Laryngeal Cancer Risk. In International journal of environmental research and public health, 18, . doi:10.3390/ijerph18147486. https://pubmed.ncbi.nlm.nih.gov/34299935/
2. França, Monica Malheiros, Mendonca, Berenice Bilharinho. 2021. Genetics of ovarian insufficiency and defects of folliculogenesis. In Best practice & research. Clinical endocrinology & metabolism, 36, 101594. doi:10.1016/j.beem.2021.101594. https://pubmed.ncbi.nlm.nih.gov/34794894/
3. Cichocka, Edyta, Górczyńska-Kosiorz, Sylwia, Misiołek, Maciej, Stryjewska-Makuch, Grażyna, Gumprecht, Janusz. 2024. DIAPH2 gene polymorphisms and laryngeal cancer risk in men. In Journal of cancer research and clinical oncology, 150, 295. doi:10.1007/s00432-024-05820-4. https://pubmed.ncbi.nlm.nih.gov/38844723/
4. Kostrzewska-Poczekaj, M, Byzia, E, Soloch, N, Szyfter, K, Giefing, M. . DIAPH2 alterations increase cellular motility and may contribute to the metastatic potential of laryngeal squamous cell carcinoma. In Carcinogenesis, 40, 1251-1259. doi:10.1093/carcin/bgz035. https://pubmed.ncbi.nlm.nih.gov/30793164/
5. Chiereghin, Chiara, Robusto, Michela, Lewis, Morag A, Asselta, Rosanna, Soldà, Giulia. 2023. In-depth genetic and molecular characterization of diaphanous related formin 2 (DIAPH2) and its role in the inner ear. In PloS one, 18, e0273586. doi:10.1371/journal.pone.0273586. https://pubmed.ncbi.nlm.nih.gov/36689403/
6. Grueb, Saskia S, Muhs, Stefanie, Popp, Yannes, Lin, Yuan-Na, Windhorst, Sabine. 2019. The formin Drosophila homologue of Diaphanous2 (Diaph2) controls microtubule dynamics in colorectal cancer cells independent of its FH2-domain. In Scientific reports, 9, 5352. doi:10.1038/s41598-019-41731-y. https://pubmed.ncbi.nlm.nih.gov/30926831/
7. Labat-de-Hoz, Leticia, Alonso, Miguel A. 2021. Formins in Human Disease. In Cells, 10, . doi:10.3390/cells10102554. https://pubmed.ncbi.nlm.nih.gov/34685534/
8. Chiereghin, Chiara, Robusto, Michela, Massa, Valentina, Asselta, Rosanna, Soldà, Giulia. 2022. Role of Cytoskeletal Diaphanous-Related Formins in Hearing Loss. In Cells, 11, . doi:10.3390/cells11111726. https://pubmed.ncbi.nlm.nih.gov/35681420/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest