C57BL/6JCya-Cep89em1flox/Cya
Common Name:
Cep89-flox
Product ID:
S-CKO-18781
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cep89-flox
Strain ID
CKOCMP-72140-Cep89-B6J-VB
Gene Name
Product ID
S-CKO-18781
Gene Alias
2610507L03Rik; Ccdc123
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cep89em1flox/Cya mice (Catalog S-CKO-18781) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000079414
NCBI RefSeq
NM_028120
Target Region
Exon 5
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
CEP89, also known as centrosomal protein 89, is essential for ciliogenesis, mitochondrial metabolism, and neuronal function. It is involved in pathways related to the assembly of primary cilia, regulation of mitochondrial complex IV activity, and synaptic transmission. It has overall biological importance in processes like cell development and cognitive function, and genetic models such as Drosophila are valuable for its study [2,3,4].
In ovarian cancer, CEP89 is overexpressed, with more than a quarter of patients having copy number gains in the gene. High expression is associated with poor prognosis, as patients with high expression have over a year shorter overall survival compared to those with low expression. In vitro studies on OC cell lines also show differences in CEP89 levels. Additionally, CEP89 is required for mitochondrial metabolism; in Drosophila, its knockdown decreases complex IV activity and is lethal. In cilia formation, CEP89 selectively functions in RAB34+ ciliary vesicle recruitment. Knockout studies in Drosophila show that it is essential for IFT-dependent neuronal cilia formation [1,2,3,4].
In conclusion, CEP89 is crucial for ciliogenesis, mitochondrial metabolism, and neuronal function. Model-based research, including Drosophila knockdown models, has revealed its role in these processes. In ovarian cancer, CEP89's overexpression and copy number gains suggest it may serve as a prognostic predictor and a potential therapeutic target.
References:
1. Berkel, Caglar, Cacan, Ercan. 2022. Copy number and expression of CEP89, a protein required for ciliogenesis, are increased and predict poor prognosis in patients with ovarian cancer. In Cell biochemistry and function, 40, 298-309. doi:10.1002/cbf.3694. https://pubmed.ncbi.nlm.nih.gov/35285957/
2. van Bon, Bregje W M, Oortveld, Merel A W, Nijtmans, Leo G, de Vries, Bert B A, Schenck, Annette. 2013. CEP89 is required for mitochondrial metabolism and neuronal function in man and fly. In Human molecular genetics, 22, 3138-51. doi:10.1093/hmg/ddt170. https://pubmed.ncbi.nlm.nih.gov/23575228/
3. Kanie, Tomoharu, Love, Julia F, Fisher, Saxton D, Gustavsson, Anna-Karin, Jackson, Peter K. 2023. A hierarchical pathway for assembly of the distal appendages that organize primary cilia. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.01.06.522944. https://pubmed.ncbi.nlm.nih.gov/36711481/
4. Hou, Yanan, Zheng, Shirui, Wu, Zhimao, Durand, Bénédicte, Wei, Qing. 2023. Drosophila transition fibers are essential for IFT-dependent ciliary elongation but not basal body docking and ciliary budding. In Current biology : CB, 33, 727-736.e6. doi:10.1016/j.cub.2022.12.046. https://pubmed.ncbi.nlm.nih.gov/36669498/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen