C57BL/6JCya-Dgkgem1flox/Cya
Common Name:
Dgkg-flox
Product ID:
S-CKO-18954
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Dgkg-flox
Strain ID
CKOCMP-110197-Dgkg-B6J-VB
Gene Name
Product ID
S-CKO-18954
Gene Alias
2900055E17Rik; 90kDa; Dagk3; E430001K23Rik; mKIAA4131
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dgkgem1flox/Cya mice (Catalog S-CKO-18954) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000089925
NCBI RefSeq
NM_138650
Target Region
Exon 4~5
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Dgkg, or diacylglycerol kinase gamma, is a key enzyme in glycerophospholipid metabolism and phosphatidylinositol signaling pathways [8]. It plays significant roles in various biological processes, and its study via genetic models can provide insights into its functions.
In hepatocellular carcinoma (HCC), endothelial Dgkg promotes tumor angiogenesis and immunosuppressive regulatory T-cell differentiation. Hypoxia-induced HIF-1α activates Dgkg transcription, and upregulated Dgkg promotes HCC progression through the ZEB2/TGF-β1 axis. Targeting endothelial Dgkg potentiates the efficiency of dual blockade of PD-1 and VEGFR-2 [1].
In Wagyu cattle, a deleterious homozygous missense variant in Dgkg is associated with hepatic fibrinogen storage disease [2]. In an epileptic patient, a heterozygous mutation in Dgkg was identified, and an induced pluripotent stem cell line was generated for studying epilepsy mechanisms [3].
In oral squamous cell carcinoma (OSCC), Dgkg is among genes in a Cox model that can predict patient prognosis, and histone lysine lactylation-induced Dgkg may be involved in OSCC drug therapy and tumor microenvironment [4].
In acute myeloid leukemia (AML), high Dgkg expression is associated with a more favorable prognosis [5].
In colorectal cancer, Dgkg promoter is hypermethylated, suppressing its expression. Ectopic expression of Dgkg suppresses cell migration and invasion, suggesting a tumor-suppressor role [6].
In GABAergic interneurons, Dgkg is dominantly expressed in somatostatin-expressing neurons, and its functional study indicates an alteration in neurite outgrowth [7].
In glioblastoma, a hypoxia-induced alternative splicing transcript Dgkg-Δ exon13 promotes glioblastoma proliferation and infiltration [9].
In conclusion, Dgkg is involved in multiple biological processes and diseases. Its study through various models, such as in disease-associated genetic variants or in vitro and in vivo functional studies, helps understand its role in tumor angiogenesis, immune evasion, neurological development, and other pathological and physiological conditions.
References：
1. Zhang, Liren, Xu, Jiali, Zhou, Suiqing, Li, Qing, Wang, Xuehao. 2023. Endothelial DGKG promotes tumor angiogenesis and immune evasion in hepatocellular carcinoma. In Journal of hepatology, 80, 82-98. doi:10.1016/j.jhep.2023.10.006. https://pubmed.ncbi.nlm.nih.gov/37838036/
2. Jacinto, Joana G P, Wohlsein, Peter, Häfliger, Irene M, Grünberg, Walter, Drögemüller, Cord. 2023. A missense variant in DGKG as a recessive functional variant for hepatic fibrinogen storage disease in Wagyu cattle. In Journal of veterinary internal medicine, 37, 2631-2637. doi:10.1111/jvim.16865. https://pubmed.ncbi.nlm.nih.gov/37681469/
3. Liu, Ya-Qing, Ling, Tiao-Wen, Wang, Hong-Yan, Song, Wen-Jun, Wang, Tian-Cheng. 2022. Generation of an integration-free induced pluripotent stem cell line (LZUSHI001-A) from an epileptic patient with DGKG mutation. In Stem cell research, 61, 102768. doi:10.1016/j.scr.2022.102768. https://pubmed.ncbi.nlm.nih.gov/35421845/
4. Huang, Guangzhao, Chen, Su, He, Jialu, Guo, Zhiyong, Li, Chunjie. 2024. Histone Lysine Lactylation (Kla)-induced BCAM Promotes OSCC Progression and Cis-Platinum Resistance. In Oral diseases, 31, 1116-1132. doi:10.1111/odi.15179. https://pubmed.ncbi.nlm.nih.gov/39503345/
5. Gravina, Teresa, Boggio, Chiara Maria Teresa, Gorla, Elisa, Corà, Davide, Baldanzi, Gianluca. 2023. Role of Diacylglycerol Kinases in Acute Myeloid Leukemia. In Biomedicines, 11, . doi:10.3390/biomedicines11071877. https://pubmed.ncbi.nlm.nih.gov/37509516/
6. Kai, Masahiro, Yamamoto, Eiichiro, Sato, Akiko, Toyota, Minoru, Suzuki, Hiromu. 2017. Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer. In Molecular carcinogenesis, 56, 1743-1752. doi:10.1002/mc.22631. https://pubmed.ncbi.nlm.nih.gov/28218473/
7. Fukumoto, Keita, Tamada, Kota, Toya, Tsuyoshi, Yanagawa, Yuchio, Takumi, Toru. 2017. Identification of genes regulating GABAergic interneuron maturation. In Neuroscience research, 134, 18-29. doi:10.1016/j.neures.2017.11.010. https://pubmed.ncbi.nlm.nih.gov/29203264/
8. Taniguchi-Ponciano, Keiko, Hinojosa-Alvarez, Silvia, Hernandez-Perez, Jesus, Marrero-Rodriguez, Daniel, Mercado, Moises. 2024. Longitudinal multiomics analysis of aggressive pituitary neuroendocrine tumors: comparing primary and recurrent tumors from the same patient, reveals genomic stability and heterogeneous transcriptomic profiles with alterations in metabolic pathways. In Acta neuropathologica communications, 12, 142. doi:10.1186/s40478-024-01796-x. https://pubmed.ncbi.nlm.nih.gov/39217365/
9. Yang, Ming, Chu, Liangzhao, Lin, Shukai, Han, Feng, Liu, Jian. 2025. The alternatively spliced diacylglycerol kinase gamma-Δ exon13 transcript generated under hypoxia promotes glioblastoma progression. In Oncology research, 33, 1189-1198. doi:10.32604/or.2024.055102. https://pubmed.ncbi.nlm.nih.gov/40296900/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen