C57BL/6JCya-Ddx23em1flox/Cya
Common Name:
Ddx23-flox
Product ID:
S-CKO-19012
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ddx23-flox
Strain ID
CKOCMP-74351-Ddx23-B6J-VB
Gene Name
Product ID
S-CKO-19012
Gene Alias
3110082M05Rik; 4921506D17Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ddx23em1flox/Cya mice (Catalog S-CKO-19012) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003450
NCBI RefSeq
NM_001080981
Target Region
Exon 4~13
Size of Effective Region
~5.2 kb
Detailed Document
Overview of Gene Research
Ddx23, also known as PRP28, is a spliceosomal RNA helicase. It is crucial for pre-mRNA splicing, specifically for the pre-B to B spliceosome complex transition and release of U1 snRNP from the 5' splice site [1]. It also participates in maintaining genome stability and sensing double-stranded RNA. Moreover, Ddx23 is involved in multiple biological processes such as the innate immune response, and its functions are essential for normal cellular activities [2,3,6].
In black carp, bcDDX23 targets bcMAVS and suppresses MAVS-mediated IFN signaling, indicating its role in antiviral innate immunity [2]. In human cells, knockdown of DDX23 enhanced the replication of VSV and reduced the activation of the NF-κB and IRF3, suggesting its importance in the innate antiviral response [3]. In ovarian cancer, DDX23 was upregulated, and its silencing impeded cell proliferation/invasion in vitro and tumor growth in vivo, regulating the mRNA processing of FOXM1 [5]. In addition, in the context of foot-and-mouth disease virus, DDX23 negatively affects virus IRES-dependent translation [4].
In summary, Ddx23 plays essential roles in pre-mRNA splicing, innate immune responses, and in certain disease conditions such as ovarian cancer and viral infections. Functional studies, including those using knockdown models, have revealed its importance in regulating biological processes and its potential as a target for antiviral drugs and cancer therapies [2,3,4,5].
References:
1. Segovia, Danilo, Adams, Dexter W, Hoffman, Nickolas, Joshua-Tor, Leemor, Krainer, Adrian R. 2024. SRSF1 interactome determined by proximity labeling reveals direct interaction with spliceosomal RNA helicase DDX23. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2322974121. doi:10.1073/pnas.2322974121. https://pubmed.ncbi.nlm.nih.gov/38743621/
2. Qin, Wei, Liu, Yankai, Xiao, Jun, Zhang, Yongan, Feng, Hao. 2023. DDX23 of black carp negatively regulates MAVS-mediated antiviral signaling in innate immune activation. In Developmental and comparative immunology, 146, 104727. doi:10.1016/j.dci.2023.104727. https://pubmed.ncbi.nlm.nih.gov/37164277/
3. Ruan, Jie, Cao, Yange, Ling, Tao, Xu, Anlong, Yuan, Shaochun. 2019. DDX23, an Evolutionary Conserved dsRNA Sensor, Participates in Innate Antiviral Responses by Pairing With TRIF or MAVS. In Frontiers in immunology, 10, 2202. doi:10.3389/fimmu.2019.02202. https://pubmed.ncbi.nlm.nih.gov/31620127/
4. Abdullah, Sahibzada Waheed, Han, Shichong, Wu, Jin'en, Sun, Shiqi, Guo, Huichen. 2020. The DDX23 Negatively Regulates Translation and Replication of Foot-and-Mouth Disease Virus and Is Degraded by 3C Proteinase. In Viruses, 12, . doi:10.3390/v12121348. https://pubmed.ncbi.nlm.nih.gov/33255534/
5. Zhao, Chen, Li, Yingwei, Qiu, Chunping, Song, Kun, Kong, Beihua. 2021. Splicing Factor DDX23, Transcriptionally Activated by E2F1, Promotes Ovarian Cancer Progression by Regulating FOXM1. In Frontiers in oncology, 11, 749144. doi:10.3389/fonc.2021.749144. https://pubmed.ncbi.nlm.nih.gov/34966670/
6. Burns, William, Bird, Lynne M, Heron, Delphine, Louie, Raymond J, Lyons, Michael J. 2021. Syndromic neurodevelopmental disorder associated with de novo variants in DDX23. In American journal of medical genetics. Part A, 185, 2863-2872. doi:10.1002/ajmg.a.62359. https://pubmed.ncbi.nlm.nih.gov/34050707/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen