C57BL/6JCya-Stumem1flox/Cya
Common Name:
Stum-flox
Product ID:
S-CKO-19058
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Stum-flox
Strain ID
CKOCMP-381310-Stum-B6J-VA
Gene Name
Product ID
S-CKO-19058
Gene Alias
6330403A02Rik; Gm10001
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Stumem1flox/Cya mice (Catalog S-CKO-19058) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000136521
NCBI RefSeq
NM_001081227
Target Region
Exon 2
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Stum, short for stumble, is an evolutionarily conserved gene with orthologs in animals from nematodes to mammals. It contains two transmembrane domains and is essential for mechanical sensing in a specific subpopulation of Drosophila proprioceptive neurons that sense joint angles. These neurons' dendrites are stretched by joint movements, and Stum is involved in transducing these mechanical stimuli [1]. It is also associated with the mechanotransduction pathway, as the stum-expressing neurons express the mechanosensory channel NOMPC, and in nompC mutants, responses to joint position are abolished [3].
In Drosophila, a mutant stum 9-3 was initially thought to affect Stum but was later found to be a new allele of O-fut1. This mutant impacts the proliferation and differentiation of Drosophila adult intestinal stem cells through the conserved Notch signaling pathway, upstream of the S2 cleavage of the Notch receptor [2].
In summary, Stum plays a crucial role in mechanical sensing in proprioceptive neurons in Drosophila, and its study, especially through Drosophila genetic models, helps in understanding the molecular mechanisms underlying proprioception. Although no KO/CKO mouse models related to Stum are mentioned in the provided references, the Drosophila models offer insights into its functions, which could potentially be relevant for understanding similar processes in mammals.
References:
1. Desai, Bela S, Chadha, Abhishek, Cook, Boaz. . The stum gene is essential for mechanical sensing in proprioceptive neurons. In Science (New York, N.Y.), 343, 1256-9. doi:10.1126/science.1247761. https://pubmed.ncbi.nlm.nih.gov/24626929/
2. Shi, Lin, Kong, Ruiyan, Li, Zhengran, Li, Jing, Li, Zhouhua. 2021. Identification of a new allele of O-fucosyltransferase 1 involved in Drosophila intestinal stem cell regulation. In Biology open, 10, . doi:10.1242/bio.058910. https://pubmed.ncbi.nlm.nih.gov/34731235/
3. Chadha, Abhishek, Kaneko, Maki, Cook, Boaz. 2015. NOMPC-dependent mechanotransduction shapes the dendrite of proprioceptive neurons. In Neuroscience letters, 597, 111-6. doi:10.1016/j.neulet.2015.04.033. https://pubmed.ncbi.nlm.nih.gov/25916878/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen