C57BL/6JCya-Fmnl1em1flox/Cya
Common Name:
Fmnl1-flox
Product ID:
S-CKO-19153
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fmnl1-flox
Strain ID
CKOCMP-57778-Fmnl1-B6J-VB
Gene Name
Product ID
S-CKO-19153
Gene Alias
8030453N10Rik; Fmnl; Fnrl; Frls
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fmnl1em1flox/Cya mice (Catalog S-CKO-19153) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000042286
NCBI RefSeq
NM_001077698
Target Region
Exon 4~6
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Fmnl1, formin-like 1, belongs to the formin family and is responsible for cytoskeletal remodeling, playing a role in various biological processes including cell migration, proliferation, and the immune response [1,2,3,5,6,7,9]. It is involved in multiple signaling pathways such as those related to CXCR2, TGF-β1, and Rac1 [1,3,7].
In cancer, FMNL1 has been extensively studied. In clear cell renal cell carcinoma (ccRCC), its upregulation promotes metastasis via induction of CXCR2, and high expression correlates with advanced tumor stage and poor prognosis [1]. In nasopharyngeal carcinoma (NPC), high FMNL1 expression is associated with aggressive disease, and it enhances cell aggressiveness by epigenetically upregulating MTA1 [2]. In non-small cell lung cancer (NSCLC), reducing FMNL1 expression suppresses cell proliferation, migration, invasion, and bone metastasis by reducing TGF-β1 expression [3]. In leukemia cells, FMNL1 depletion reduces cell proliferation, colony formation, and migration [7]. In glioblastoma multiforme (GBM), FMNL1 is an independent predictor of poor prognosis, and its downregulation suppresses cell migration and invasion [6]. In breast adenocarcinoma, FMNL1 is required for efficient cell migration, and its γ-isoform enhances cell adhesion and migration by bundling filamentous actin [9]. In gastric cancer, elevated FMNL1 is associated with larger tumor size and higher disease stage [10]. Also, FMNL1 is a biomarker for tumor-infiltrating immune cells and is associated with a good immunotherapeutic response [4].
In T cells, FMNL1 contributes to efficient migration by promoting deformation of the rigid T cell nucleus in confined environments [5]. In membranous nephropathy and other glomerular diseases, anti-FMNL1 IgG4 antibodies are found, which may be related to disease progression [8].
In conclusion, FMNL1 is crucial for cytoskeletal remodeling and has significant impacts on cancer progression, immune cell function, and disease processes in various tissues. Studies using gene knockdown and other loss-of-function models have revealed its role in promoting cancer cell migration, invasion, and proliferation, highlighting its potential as a prognostic factor and therapeutic target in multiple cancers.
References:
1. Zhang, Mei-Fang, Li, Qiu-Li, Yang, Yu-Feng, Cao, Yun, Zhang, Chris Zhiyi. 2020. FMNL1 Exhibits Pro-Metastatic Activity via CXCR2 in Clear Cell Renal Cell Carcinoma. In Frontiers in oncology, 10, 564614. doi:10.3389/fonc.2020.564614. https://pubmed.ncbi.nlm.nih.gov/33324547/
2. Chen, Wen-Hui, Cai, Mu-Yan, Zhang, Jia-Xing, Qian, Chao-Nan, Xie, Dan. 2018. FMNL1 mediates nasopharyngeal carcinoma cell aggressiveness by epigenetically upregulating MTA1. In Oncogene, 37, 6243-6258. doi:10.1038/s41388-018-0351-8. https://pubmed.ncbi.nlm.nih.gov/30013189/
3. Yang, Xing-Yi, Liao, Jun-Jie, Xue, Wu-Rong. 2019. FMNL1 down-regulation suppresses bone metastasis through reducing TGF-β1 expression in non-small cell lung cancer (NSCLC). In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 117, 109126. doi:10.1016/j.biopha.2019.109126. https://pubmed.ncbi.nlm.nih.gov/31387165/
4. Lu, Guomin, Wang, Hui, Xu, Rui, Zhan, Qiang, Zhang, Qinglin. 2023. Formin protein FMNL1 is a biomarker for tumor-infiltrating immune cells and associated with well immunotherapeutic response. In Journal of Cancer, 14, 2978-2989. doi:10.7150/jca.86965. https://pubmed.ncbi.nlm.nih.gov/37859818/
5. Sigler, Ashton L, Thompson, Scott B, Ellwood-Digel, Logan, Del Alamo, Juan C, Jacobelli, Jordan. 2024. FMNL1 and mDia1 promote efficient T cell migration through complex environments via distinct mechanisms. In Frontiers in immunology, 15, 1467415. doi:10.3389/fimmu.2024.1467415. https://pubmed.ncbi.nlm.nih.gov/39430739/
6. Higa, Nayuta, Shinsato, Yoshinari, Kamil, Muhammad, Yoshimoto, Koji, Arita, Kazunori. 2019. Formin-like 1 (FMNL1) Is Associated with Glioblastoma Multiforme Mesenchymal Subtype and Independently Predicts Poor Prognosis. In International journal of molecular sciences, 20, . doi:10.3390/ijms20246355. https://pubmed.ncbi.nlm.nih.gov/31861134/
7. Favaro, Patricia, Traina, Fabiola, Machado-Neto, João Agostinho, Ridley, Anne J, Saad, Sara Teresinha Olalla. 2013. FMNL1 promotes proliferation and migration of leukemia cells. In Journal of leukocyte biology, 94, 503-12. doi:10.1189/jlb.0113057. https://pubmed.ncbi.nlm.nih.gov/23801653/
8. Bruschi, Maurizio, Cavalli, Andrea, Moll, Solange, Ghiggeri, Gian Marco, Prunotto, Marco. 2022. Discovery of anti-Formin-like 1 protein (FMNL1) antibodies in membranous nephropathy and other glomerular diseases. In Scientific reports, 12, 13659. doi:10.1038/s41598-022-17696-w. https://pubmed.ncbi.nlm.nih.gov/35953506/
9. Miller, Eric W, Blystone, Scott D. 2019. The carboxy-terminus of the formin FMNL1ɣ bundles actin to potentiate adenocarcinoma migration. In Journal of cellular biochemistry, 120, 14383-14404. doi:10.1002/jcb.28694. https://pubmed.ncbi.nlm.nih.gov/30977161/
10. Mansuri, Naziha, Heuser, Vanina D, Birkman, Eva-Maria, Carpén, Olli, Lehtinen, Laura. 2021. FHOD1 and FMNL1 formin proteins in intestinal gastric cancer: correlation with tumor-infiltrating T lymphocytes and molecular subtypes. In Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association, 24, 1254-1263. doi:10.1007/s10120-021-01203-7. https://pubmed.ncbi.nlm.nih.gov/34115237/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen