C57BL/6JCya-Zcwpw1em1flox/Cya
Common Name:
Zcwpw1-flox
Product ID:
S-CKO-19222
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Zcwpw1-flox
Strain ID
CKOCMP-381678-Zcwpw1-B6J-VB
Gene Name
Product ID
S-CKO-19222
Gene Alias
Gm1053
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zcwpw1em1flox/Cya mice (Catalog S-CKO-19222) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000035852
NCBI RefSeq
NM_001005426
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Zcwpw1, with no common aliases provided, is an essential factor in meiotic processes. It is recruited to recombination hotspots by PRDM9 and plays a crucial role in meiotic double-strand break repair, which is vital for homologous chromosomes to pair and recombine, ensuring balanced segregation and genetic diversity in sexual reproduction [1,2,4,5,7]. This process is integral to the formation of sex cells.
Male Zcwpw1 knockout mice show severe DSB repair and synapsis defects, leading to sterility. Their spermatocytes are arrested at the pachytene-like stage, phenocopying the knockout mice. In contrast, in female mice, deletion of Zcwpw1 only slows down meiosis prophase I progression, and they can still maintain normal fertility until mid-adulthood [1,2,4]. A loss-of-function variant in ZCWPW1 in humans has also been reported to cause male infertility with sperm head defect and high DNA fragmentation [6]. Additionally, Zcwpw1 has been associated with Alzheimer's disease risk, as rare-variant burden in it highlighted this gene as a potential driver of an AD-genome-wide association study locus [3].
In conclusion, Zcwpw1 is indispensable for meiotic synapsis in males, playing a key role in the production of male sex cells. Mouse knockout models have been instrumental in revealing its role in male fertility. Its association with Alzheimer's disease also indicates its potential importance in neurodegenerative disease research, although further study is needed to fully understand this connection.
References:
1. Wells, Daniel, Bitoun, Emmanuelle, Moralli, Daniela, Green, Catherine, Myers, Simon R. 2020. ZCWPW1 is recruited to recombination hotspots by PRDM9 and is essential for meiotic double strand break repair. In eLife, 9, . doi:10.7554/eLife.53392. https://pubmed.ncbi.nlm.nih.gov/32744506/
2. Huang, Tao, Yuan, Shenli, Gao, Lei, Chen, Zi-Jiang, Liu, Hongbin. 2020. The histone modification reader ZCWPW1 links histone methylation to PRDM9-induced double-strand break repair. In eLife, 9, . doi:10.7554/eLife.53459. https://pubmed.ncbi.nlm.nih.gov/32374261/
3. Holstege, Henne, Hulsman, Marc, Charbonnier, Camille, Bellenguez, Céline, Lambert, Jean-Charles. 2022. Exome sequencing identifies rare damaging variants in ATP8B4 and ABCA1 as risk factors for Alzheimer's disease. In Nature genetics, 54, 1786-1794. doi:10.1038/s41588-022-01208-7. https://pubmed.ncbi.nlm.nih.gov/36411364/
4. Li, Miao, Huang, Tao, Li, Meng-Jing, Liu, Hong-Bin, Liu, Kui. 2019. The histone modification reader ZCWPW1 is required for meiosis prophase I in male but not in female mice. In Science advances, 5, eaax1101. doi:10.1126/sciadv.aax1101. https://pubmed.ncbi.nlm.nih.gov/31453335/
5. Yuan, Shenli, Huang, Tao, Bao, Ziyou, Liu, Hongbin, Chen, Zi-Jiang. 2022. The histone modification reader ZCWPW1 promotes double-strand break repair by regulating cross-talk of histone modifications and chromatin accessibility at meiotic hotspots. In Genome biology, 23, 187. doi:10.1186/s13059-022-02758-z. https://pubmed.ncbi.nlm.nih.gov/36068616/
6. Song, Yuelin, Guo, Juncen, Zhou, Yanling, Zhang, Guohui, Wang, Hongjing. 2024. A loss-of-function variant in ZCWPW1 causes human male infertility with sperm head defect and high DNA fragmentation. In Reproductive health, 21, 18. doi:10.1186/s12978-024-01746-9. https://pubmed.ncbi.nlm.nih.gov/38310235/
7. Mahgoub, Mohamed, Paiano, Jacob, Bruno, Melania, Nussenzweig, André, Macfarlan, Todd S. 2020. Dual histone methyl reader ZCWPW1 facilitates repair of meiotic double strand breaks in male mice. In eLife, 9, . doi:10.7554/eLife.53360. https://pubmed.ncbi.nlm.nih.gov/32352380/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen