C57BL/6JCya-Kyat1em1flox/Cya
Common Name:
Kyat1-flox
Product ID:
S-CKO-19236
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Kyat1-flox
Strain ID
CKOCMP-70266-Kyat1-B6J-VB
Gene Name
Product ID
S-CKO-19236
Gene Alias
2010009K05Rik; Ccbl1; Gtk; Kat1; KatI
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kyat1em1flox/Cya mice (Catalog S-CKO-19236) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000113663
NCBI RefSeq
NM_172404
Target Region
Exon 5~14
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
KYAT1, also known as kynurenine aminotransferase 1 or CCBL1, is a bi-functional enzyme. It plays a major role in Se-methylselenocysteine (MSC) metabolism, catalyzing transamination and beta-elimination activity with MSC as a substrate to produce methylselenol, a cytotoxic selenium metabolite causing apoptosis in cancer cells [2]. It is also involved in the kynurenine pathway, which is related to the metabolism of tryptophan and the production of neuroactive metabolites like kynurenic acid and quinolinic acid [5].
In a study on the effect of a heavy-metal mixture on neurological damage in rats, joint analysis of proteome and metabolome suggested that KYAT1 may participate in the neurological impairments induced by the heavy-metal mixture [1]. In patients with Prader-Willi syndrome, KYAT1 exhibited differential expression in liver steatosis, with a progressive increase from grade 1 to grade 3, indicating its potential as a biomarker for this condition [3]. In critical illness, the mRNA expression of KYAT1 in skeletal muscle was affected, and lower expression was associated with a lower risk of weakness [4].
In conclusion, KYAT1 is involved in important metabolic pathways such as the metabolism of selenium-containing compounds and the kynurenine pathway. Its role has been implicated in various disease-related processes including neurological impairments, hepatic steatosis in Prader-Willi syndrome, and critical illness-related muscle conditions, highlighting its significance in understanding these biological and disease states.
References:
1. Xie, Jie, Zhou, Fankun, Ouyang, Lu, Feng, Chang, Fan, Guangqin. 2023. Insight into the effect of a heavy metal mixture on neurological damage in rats through combined serum metabolomic and brain proteomic analyses. In The Science of the total environment, 895, 165009. doi:10.1016/j.scitotenv.2023.165009. https://pubmed.ncbi.nlm.nih.gov/37353033/
2. Selvam, Arun Kumar, Björnstedt, Mikael. 2020. A Novel Assay Method to Determine the β-Elimination of Se-Methylselenocysteine to Monomethylselenol by Kynurenine Aminotransferase 1. In Antioxidants (Basel, Switzerland), 9, . doi:10.3390/antiox9020139. https://pubmed.ncbi.nlm.nih.gov/32033380/
3. Pascut, Devis, Giraudi, Pablo J, Banfi, Cristina, Grugni, Graziano, Sartorio, Alessandro. 2023. Proteome profiling identifies circulating biomarkers associated with hepatic steatosis in subjects with Prader-Willi syndrome. In Frontiers in endocrinology, 14, 1254778. doi:10.3389/fendo.2023.1254778. https://pubmed.ncbi.nlm.nih.gov/38034016/
4. Vanhorebeek, Ilse, Gunst, Jan, Casaer, Michaël P, Gosselink, Rik, Van den Berghe, Greet. 2023. Skeletal Muscle Myokine Expression in Critical Illness, Association With Outcome and Impact of Therapeutic Interventions. In Journal of the Endocrine Society, 7, bvad001. doi:10.1210/jendso/bvad001. https://pubmed.ncbi.nlm.nih.gov/36726836/
5. Brown, Samara J, Brown, Amelia M, Purves-Tyson, Tertia D, Shannon Weickert, Cynthia, Newell, Kelly A. 2021. Alterations in the kynurenine pathway and excitatory amino acid transporter-2 in depression with and without psychosis: Evidence of a potential astrocyte pathology. In Journal of psychiatric research, 147, 203-211. doi:10.1016/j.jpsychires.2021.12.039. https://pubmed.ncbi.nlm.nih.gov/35063739/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen