C57BL/6JCya-Hes7em1flox/Cya
Common Name:
Hes7-flox
Product ID:
S-CKO-19254
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Hes7-flox
Strain ID
CKOCMP-84653-Hes7-B6J-VB
Gene Name
Product ID
S-CKO-19254
Gene Alias
bHLHb37
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hes7em1flox/Cya mice (Catalog S-CKO-19254) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024543
NCBI RefSeq
NM_033041
Target Region
Exon 2~4
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Hes7, a basic helix-loop-helix (bHLH)-type repressor gene, is regulated by Notch and expressed in the presomitic mesoderm [6]. It is a key segmentation clock gene that controls the periodic formation of somites, which are crucial for the proper development of the vertebrae [1]. Somitogenesis, regulated by the oscillatory expression of Hes7, is associated with the Fgf and Notch signaling pathways [2].
Mouse models have provided significant insights into Hes7 function. In knock-in mice where Hes7 3'UTR was disrupted, proper splicing was affected, leading to reduced Hes7 protein levels, damped oscillation, and improper periodic somite segmentation, demonstrating the importance of 3'UTR for normal Hes7 function [5]. Mice expressing mutant Hes7 with a longer half-life showed severely disorganized somite segmentation and oscillatory expression after a few normal cycles, highlighting the essential role of Hes7 protein instability for sustained oscillation and its function as a segmentation clock [7]. Additionally, Fgf4 mutants, which display vertebral defects, show that Fgf4 maintains Hes7 levels and normal oscillatory patterns, indicating a genetic synergy between Hes7 and Fgf4 [4].
In conclusion, Hes7 is essential for the somite segmentation clock during vertebrate development. Mouse models, including those with disrupted 3'UTR or altered protein stability, have revealed its role in normal somitogenesis. Understanding Hes7 function is relevant to human congenital spine diseases, such as spondylocostal dysostosis 4, caused by HES7 variants, emphasizing the importance of Hes7-related research for disease understanding [3].
References:
1. Sanaki-Matsumiya, Marina, Kageyama, Ryoichiro. . Time-Lapse Bioluminescence Imaging of Hes7 Expression In Vitro and Ex Vivo. In Methods in molecular biology (Clifton, N.J.), 2525, 321-332. doi:10.1007/978-1-0716-2473-9_25. https://pubmed.ncbi.nlm.nih.gov/35836080/
2. Harima, Yukiko, Kageyama, Ryoichiro. 2013. Oscillatory links of Fgf signaling and Hes7 in the segmentation clock. In Current opinion in genetics & development, 23, 484-90. doi:10.1016/j.gde.2013.02.005. https://pubmed.ncbi.nlm.nih.gov/23465881/
3. Lv, Shaoguang, Wu, Yuanyuan, Liu, Fang, Jiao, Baoquan. 2023. A novel homozygous HES7 splicing variant causing spondylocostal dysostosis 4: a case report. In Frontiers in pediatrics, 11, 1201999. doi:10.3389/fped.2023.1201999. https://pubmed.ncbi.nlm.nih.gov/37691774/
4. Anderson, Matthew J, Magidson, Valentin, Kageyama, Ryoichiro, Lewandoski, Mark. 2020. Fgf4 maintains Hes7 levels critical for normal somite segmentation clock function. In eLife, 9, . doi:10.7554/eLife.55608. https://pubmed.ncbi.nlm.nih.gov/33210601/
5. Fujimuro, Takeshi, Matsui, Takaaki, Nitanda, Yasuhide, Nakahata, Yasukazu, Bessho, Yasumasa. 2014. Hes7 3'UTR is required for somite segmentation function. In Scientific reports, 4, 6462. doi:10.1038/srep06462. https://pubmed.ncbi.nlm.nih.gov/25248974/
6. Bessho, Y, Miyoshi, G, Sakata, R, Kageyama, R. . Hes7: a bHLH-type repressor gene regulated by Notch and expressed in the presomitic mesoderm. In Genes to cells : devoted to molecular & cellular mechanisms, 6, 175-85. doi:. https://pubmed.ncbi.nlm.nih.gov/11260262/
7. Hirata, Hiromi, Bessho, Yasumasa, Kokubu, Hiroshi, Lewis, Julian, Kageyama, Ryoichiro. 2004. Instability of Hes7 protein is crucial for the somite segmentation clock. In Nature genetics, 36, 750-4. doi:. https://pubmed.ncbi.nlm.nih.gov/15170214/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen