C57BL/6NCya-Prmt9em1flox/Cya
Common Name:
Prmt9-flox
Product ID:
S-CKO-19911
Background:
C57BL/6NCya
Product Type
Age
Genotype
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Basic Information
Strain Name
Prmt9-flox
Strain ID
CKOCMP-102182-Prmt9-B6N-VA
Gene Name
Product ID
S-CKO-19911
Gene Alias
Prmt10
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Prmt9em1flox/Cya mice (Catalog S-CKO-19911) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000056237
NCBI RefSeq
NM_001081240
Target Region
Exon 2
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Prmt9, also known as FBXO11, is a protein arginine N-methyltransferase. It belongs to the PRMT family and is involved in various biological processes such as RNA translation, DNA damage response, innate antiviral immune response, and RNA splicing. It catalyzes arginine methylation, which modulates the function of its target proteins and is important for maintaining normal cellular functions [1,2,3,4].
In a Prmt9 conditional knockout (cKO) mouse model, knockout of Prmt9 in hippocampal neurons led to alternative splicing of about 1900 genes. This resulted in aberrant synapse development and impaired learning and memory, suggesting Prmt9's crucial role in neuronal development through regulating RNA splicing [3]. In acute myeloid leukemia (AML), targeting Prmt9 eliminated the disease via cancer-intrinsic mechanisms and cancer-extrinsic type I interferon-associated immunity. Prmt9 ablation in AML cells decreased the arginine methylation of regulators of RNA translation and DNA damage response, suppressing cell survival [1].
In conclusion, Prmt9 is essential for multiple biological processes, especially RNA-related functions and immune regulation. The Prmt9 cKO mouse model has been instrumental in revealing its role in neuronal development, while studies in AML highlight its potential as an anticancer target. These findings contribute to our understanding of disease mechanisms and potential therapeutic strategies.
References:
1. Dong, Haojie, He, Xin, Zhang, Lei, Luo, Yun Lyna, Li, Ling. 2024. Targeting PRMT9-mediated arginine methylation suppresses cancer stem cell maintenance and elicits cGAS-mediated anticancer immunity. In Nature cancer, 5, 601-624. doi:10.1038/s43018-024-00736-x. https://pubmed.ncbi.nlm.nih.gov/38413714/
2. Bai, Xuemei, Sui, Chao, Liu, Feng, Liu, Bingyu, Gao, Chengjiang. 2022. The protein arginine methyltransferase PRMT9 attenuates MAVS activation through arginine methylation. In Nature communications, 13, 5016. doi:10.1038/s41467-022-32628-y. https://pubmed.ncbi.nlm.nih.gov/36028484/
3. Shen, Lei, Ma, Xiaokuang, Wang, Yuanyuan, Xing, Yi, Yang, Yanzhong. 2024. Loss-of-function mutation in PRMT9 causes abnormal synapse development by dysregulation of RNA alternative splicing. In Nature communications, 15, 2809. doi:10.1038/s41467-024-47107-9. https://pubmed.ncbi.nlm.nih.gov/38561334/
4. Yang, Yanzhong, Hadjikyriacou, Andrea, Xia, Zheng, Clarke, Steven G, Bedford, Mark T. 2015. PRMT9 is a type II methyltransferase that methylates the splicing factor SAP145. In Nature communications, 6, 6428. doi:10.1038/ncomms7428. https://pubmed.ncbi.nlm.nih.gov/25737013/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen