C57BL/6NCya-Cxcr5em1/Cya
Common Name
Cxcr5-KO
Product ID
S-KO-01231
Backgroud
C57BL/6NCya
Strain ID
KOCMP-12145-Cxcr5-B6N-VA
Status
When using this mouse strain in a publication, please cite “Cxcr5-KO Mouse (Catalog S-KO-01231) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Cxcr5-KO
Strain ID
KOCMP-12145-Cxcr5-B6N-VA
Gene Name
Product ID
S-KO-01231
Gene Alias
Blr1, Gpcr6, MDR15, CXC-R5, CXCR-5
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 9
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000062215
NCBI RefSeq
NM_007551
Target Region
Exon 1~2
Size of Effective Region
~13.1 kb
Overview of Gene Research
CXCR5, a G-protein coupled receptor, binds to its ligand CXCL13, building a crucial signaling network. This interaction is involved in lymphoid neogenesis, lymphoid organization, and immune responses [1,2]. The CXCL13/CXCR5 axis also contributes to the pathogenesis of several autoimmune diseases, inflammatory diseases, and cancers [1-3].
In a mouse model of sepsis-associated encephalopathy, knocking out CXCR5 enhanced autophagy and partially reversed cognitive deficits, suggesting that CXCR5 may act via the p38MAPK/NF-κB/STAT3 signaling pathway to inhibit hippocampal autophagy during sepsis, contributing to cognitive dysfunction [4]. In the spinal nerve ligation (SNL) model of neuropathic pain, CXCR5 expression induced by SNL was required for SNL-induced activation of spinal astrocytes and microglia, and neuropathic pain was abrogated in Cxcr5-/-mice, indicating CXCR5's role in neuropathic pain [3].
In conclusion, CXCR5 is essential in multiple biological processes and disease conditions. Gene knockout mouse models have revealed its role in cognitive dysfunction in sepsis-associated encephalopathy and neuropathic pain. These findings contribute to understanding the underlying mechanisms of these diseases and may provide potential therapeutic targets related to CXCR5 [3,4].
References:
1. Pan, Zijian, Zhu, Tong, Liu, Yanjun, Zhang, Nannan. 2022. Role of the CXCL13/CXCR5 Axis in Autoimmune Diseases. In Frontiers in immunology, 13, 850998. doi:10.3389/fimmu.2022.850998. https://pubmed.ncbi.nlm.nih.gov/35309354/
2. Wang, Binhan, Wang, Manni, Ao, Danyi, Wei, Xiawei. 2022. CXCL13-CXCR5 axis: Regulation in inflammatory diseases and cancer. In Biochimica et biophysica acta. Reviews on cancer, 1877, 188799. doi:10.1016/j.bbcan.2022.188799. https://pubmed.ncbi.nlm.nih.gov/36103908/
3. Jiang, Bao-Chun, Cao, De-Li, Zhang, Xin, Ji, Ru-Rong, Gao, Yong-Jing. 2016. CXCL13 drives spinal astrocyte activation and neuropathic pain via CXCR5. In The Journal of clinical investigation, 126, 745-61. doi:10.1172/JCI81950. https://pubmed.ncbi.nlm.nih.gov/26752644/
4. Shen, Yanan, Zhang, Yuan, Du, Jiayue, Bao, Hongguang, Si, Yanna. 2021. CXCR5 down-regulation alleviates cognitive dysfunction in a mouse model of sepsis-associated encephalopathy: potential role of microglial autophagy and the p38MAPK/NF-κB/STAT3 signaling pathway. In Journal of neuroinflammation, 18, 246. doi:10.1186/s12974-021-02300-1. https://pubmed.ncbi.nlm.nih.gov/34711216/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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