C57BL/6JCya-Dhx15em1/Cya
Common Name:
Dhx15-KO
Product ID:
S-KO-01750
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Contact for Pricing
Basic Information
Strain Name
Dhx15-KO
Strain ID
KOCMP-13204-Dhx15-B6J-VA
Gene Name
Product ID
S-KO-01750
Gene Alias
DBP1; DEAH9; Ddx15; HRH2; mDEAH9
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dhx15em1/Cya mice (Catalog S-KO-01750) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031061
NCBI RefSeq
NM_007839
Target Region
Exon 2
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Dhx15, also known as DEAH-box helicase 15, is an ATP-dependent RNA helicase. It is mainly involved in RNA metabolism, including RNA splicing and ribosome maturation [4]. It is associated with multiple pathways such as the Wnt signaling pathway, which is crucial for various developmental processes [1]. Dhx15 is of great biological importance as it participates in numerous biological processes related to development and disease. Genetic models like zebrafish and mice are valuable for studying Dhx15.
In zebrafish, dhx15 knockout leads to profound intestinal defects, with decreased intestinal cell proliferation and increased apoptosis. The Wnt signaling pathway is down-regulated in these knockout zebrafish, indicating that dhx15 regulates zebrafish intestinal development through this pathway [1]. In T-cell acute lymphoblastic leukemia (T-ALL) murine models, DHX15 is identified as a dependency factor. Its depletion hinders burst proliferation during T-cell development, perturbs RNA splicing, and suppresses glutamine import and mTORC1 activity [2]. In Villin-Cre/KrasG12D+/- mice, knockout of Dhx15 attenuates the colorectal cancer (CRC) phenotype as Fusobacterium nucleatum promotes CRC progression by binding to DHX15 [3]. IEC-specific Dhx15-knockout mice show impaired IFN-β, IFN-λ3, and IL-18 production in response to enteric RNA viruses, resulting in uncontrolled intestinal inflammation [5]. In zebrafish, dhx15 -/- leads to perturbed hematopoietic stem/progenitor cells, with inhibited proliferation mediated through the unfolded protein response (UPR) pathway [6]. Homozygous Dhx15 gene deficiency is lethal in zebrafish and mouse embryos. In mice, it impairs liver organogenesis in embryos and liver regeneration after partial hepatectomy, and also affects liver metastasis and primary tumor growth in hepatocellular carcinoma models [7].
In conclusion, Dhx15 plays essential roles in multiple biological processes such as intestinal development, hematopoiesis, and liver development and regeneration. Through gene knockout models in zebrafish and mice, its significance in diseases like leukemia, CRC, and liver cancer has been revealed. These studies contribute to understanding the molecular mechanisms underlying these diseases and may provide potential therapeutic targets.
References:
1. Yao, Jinpeng, Cai, Yuanhua, Chen, Zhixiang, Li, Yang, Wang, Shaoyuan. 2023. Dhx15 regulates zebrafish intestinal development through the Wnt signaling pathway. In Genomics, 115, 110578. doi:10.1016/j.ygeno.2023.110578. https://pubmed.ncbi.nlm.nih.gov/36775210/
2. Guo, Hao, Xu, Jin, Xing, Peiqi, Van Vlierberghe, Pieter, Liu, Hudan. 2023. RNA helicase DHX15 exemplifies a unique dependency in acute leukemia. In Haematologica, 108, 2029-2043. doi:10.3324/haematol.2022.282066. https://pubmed.ncbi.nlm.nih.gov/36861414/
3. Zhu, Huiyuan, Li, Man, Bi, Dexi, Wei, Qing, Qin, Huanlong. 2024. Fusobacterium nucleatum promotes tumor progression in KRAS p.G12D-mutant colorectal cancer by binding to DHX15. In Nature communications, 15, 1688. doi:10.1038/s41467-024-45572-w. https://pubmed.ncbi.nlm.nih.gov/38402201/
4. Zhao, Miaomiao, Ying, Lixiong, Wang, Rusha, Chen, Zhi, Yang, Zhenggang. 2021. DHX15 Inhibits Autophagy and the Proliferation of Hepatoma Cells. In Frontiers in medicine, 7, 591736. doi:10.3389/fmed.2020.591736. https://pubmed.ncbi.nlm.nih.gov/33644083/
5. Xing, Junji, Zhou, Xiaojing, Fang, Mingli, Minze, Laurie J, Zhang, Zhiqiang. . DHX15 is required to control RNA virus-induced intestinal inflammation. In Cell reports, 35, 109205. doi:10.1016/j.celrep.2021.109205. https://pubmed.ncbi.nlm.nih.gov/34161762/
6. Cai, Yuanhua, Wang, Jing, Jin, Daqing, Zhong, Tao Peter, Wang, Shaoyuan. 2021. Dhx15 regulates zebrafish definitive hematopoiesis through the unfolded protein response pathway. In Cancer science, 112, 3884-3894. doi:10.1111/cas.15002. https://pubmed.ncbi.nlm.nih.gov/34077586/
7. Portolés, Irene, Ribera, Jordi, Fernandez-Galán, Esther, Jiménez, Wladimiro, Morales-Ruiz, Manuel. 2024. Identification of Dhx15 as a Major Regulator of Liver Development, Regeneration, and Tumor Growth in Zebrafish and Mice. In International journal of molecular sciences, 25, . doi:10.3390/ijms25073716. https://pubmed.ncbi.nlm.nih.gov/38612527/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen