C57BL/6JCya-B4galt1em1/Cya
Common Name:
B4galt1-KO
Product ID:
S-KO-02232
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
B4galt1-KO
Strain ID
KOCMP-14595-B4galt1-B6J-VA
Gene Name
Product ID
S-KO-02232
Gene Alias
B-1; 4-GalT; 4-GalT1; GalT; Ggtb; Ggtb2; b4Gal-T1; beta-1; beta4Gal-T1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-B4galt1em1/Cya mice (Catalog S-KO-02232) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030121
NCBI RefSeq
NM_022305
Target Region
Exon 2
Size of Effective Region
~0.2 kb
Detailed Document
Overview of Gene Research
B4galt1, also known as beta-1,4-galactosyltransferase1, is a key gene involved in N-glycan biosynthesis [1]. N-glycan biosynthesis is associated with various cellular processes such as protein folding, cell-cell recognition, and immune response regulation. This gene's function is crucial in maintaining normal cellular functions and its dysregulation can lead to various biological consequences.
In lung adenocarcinoma, B4galt1 is highly expressed in invasive adenocarcinoma samples compared to minimally invasive adenocarcinoma. Knockdown of B4galt1 in in vitro and in vivo experiments showed that it regulated cell proliferation, invasion, and was related to the impaired antitumour capacity of CD8 + T cells. Mechanistically, it mediates the N-linked glycosylation of PD-L1 protein, preventing its degradation, and also stabilizes the TAZ protein via glycosylation, activating CD274 transcriptionally, leading to immune escape. Inhibition of B4galt1 increased CD8 + T-cell abundance and activity and enhanced anti-PD-1 therapy's antitumour immunity [1].
In hepatocellular carcinoma, decreased B4galt1 expression was associated with enhanced cell migration, invasion, and lung metastasis. Knockdown or knockout of B4galt1 in HCC cells increased cell adhesion to laminin through modifying the N-glycans of integrins α6 and β1 [2].
In glioblastoma, lentivirus-mediated B4galt1 knockdown inhibited tumour development in vitro and in vivo, increased apoptosis and autophagy [3].
In pancreatic ductal adenocarcinomas, genetic perturbation of B4galt1 expression in cell lines regulated cancer progression and chemoresistance. Depletion of B4galt1 rescued the response of chemoresistant cells to gemcitabine in an orthotopic model [4].
In acute myeloid leukemia, silencing B4galt1 significantly promoted apoptosis [5].
In conclusion, B4galt1 plays significant roles in multiple disease conditions, mainly through its function in N-glycan biosynthesis. Studies using gene knockdown or knockout models in various cancers such as lung adenocarcinoma, hepatocellular carcinoma, glioblastoma, pancreatic ductal adenocarcinomas, and acute myeloid leukemia have revealed its contribution to cancer cell proliferation, invasion, metastasis, immune escape, chemoresistance, and apoptosis regulation. These findings highlight B4galt1 as a potential therapeutic target in these diseases.
References:
1. Cui, Yanan, Li, Jun, Zhang, Pengpeng, Zhang, Erbao, Guo, Renhua. 2023. B4GALT1 promotes immune escape by regulating the expression of PD-L1 at multiple levels in lung adenocarcinoma. In Journal of experimental & clinical cancer research : CR, 42, 146. doi:10.1186/s13046-023-02711-3. https://pubmed.ncbi.nlm.nih.gov/37303063/
2. Chen, Po-Da, Liao, Ying-Yu, Cheng, Yu-Chia, Wu, Yao-Ming, Huang, Min-Chuan. 2023. Decreased B4GALT1 promotes hepatocellular carcinoma cell invasiveness by regulating the laminin-integrin pathway. In Oncogenesis, 12, 49. doi:10.1038/s41389-023-00494-y. https://pubmed.ncbi.nlm.nih.gov/37907465/
3. Wang, Pu, Li, Xiaolong, Xie, Yuan. . B4GalT1 Regulates Apoptosis and Autophagy of Glioblastoma In Vitro and In Vivo. In Technology in cancer research & treatment, 19, 1533033820980104. doi:10.1177/1533033820980104. https://pubmed.ncbi.nlm.nih.gov/33287670/
4. Chen, Yitian, Su, Liangping, Huang, Cheng, Liu, Chao, Wong, Ping-Pui. 2020. Galactosyltransferase B4GALT1 confers chemoresistance in pancreatic ductal adenocarcinomas by upregulating N-linked glycosylation of CDK11p110. In Cancer letters, 500, 228-243. doi:10.1016/j.canlet.2020.12.006. https://pubmed.ncbi.nlm.nih.gov/33309857/
5. Ren, Zhihong, Huang, Xiaoyu, Lv, Qing, Wang, Fanping, Wang, Mingyong. 2022. High expression of B4GALT1 is associated with poor prognosis in acute myeloid leukemia. In Frontiers in genetics, 13, 882004. doi:10.3389/fgene.2022.882004. https://pubmed.ncbi.nlm.nih.gov/36568388/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen