C57BL/6JCya-Irf8em1/Cya
Common Name
Irf8-KO
Product ID
S-KO-02558
Backgroud
C57BL/6JCya
Strain ID
KOCMP-15900-Irf8-B6J-VA
Status
When using this mouse strain in a publication, please cite “Irf8-KO Mouse (Catalog S-KO-02558) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Irf8-KO
Strain ID
KOCMP-15900-Irf8-B6J-VA
Gene Name
Product ID
S-KO-02558
Gene Alias
Myls, ICSBP, IRF-8, Icsbp1
Background
C57BL/6JCya
Gene Full Name
interferon regulatory factor 8
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 8 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000047737
NCBI Transcript ID
NM_008320
Target Region
Exon 3~6
Size of Effective Region
~6.1 kb
Overview of Gene Research
Irf8, also known as interferon regulatory factor 8, is a transcription factor of the IRF protein family. It is essential for myeloid cell lineage commitment and differentiation, and is involved in various immune-related pathways such as antigen processing and presentation, cytokine-mediated activation, and immune cell development [2,5]. It also plays a role in hematopoiesis, bridging lymphoid and dendritic cell differentiation [3]. Genetic models, especially gene knockout (KO) and conditional knockout (CKO) mouse models, have been crucial in understanding its functions.
Deletion of Irf8 in mice leads to massive accumulation of CD11b+Gr1+ immature myeloid cells, particularly PMN-MDSCs [2]. In a murine breast cancer model, TAM-specific Irf8 deletion prevented exhaustion of cancer-cell-reactive CTLs and suppressed tumor growth, indicating that Irf8 in tumor-associated macrophages (TAMs) promotes CTL exhaustion in cancer [1]. In 5xFAD model mice, constitutive and postnatal Irf8 deletion reduced the interaction of microglia with amyloidβ plaques and the size of plaques, lessening neuronal loss, suggesting its importance in microglia-related functions [4].
In conclusion, Irf8 is a key transcription factor regulating both the immune and non-immune components in health and diseases. KO/CKO mouse models have revealed its role in cancer, microglia-related functions, and myeloid cell development, providing insights into the mechanisms of these biological processes and potential disease-related pathways.
References:
1. Nixon, Briana G, Kuo, Fengshen, Ji, LiangLiang, Hakimi, A Ari, Li, Ming O. 2022. Tumor-associated macrophages expressing the transcription factor IRF8 promote T cell exhaustion in cancer. In Immunity, 55, 2044-2058.e5. doi:10.1016/j.immuni.2022.10.002. https://pubmed.ncbi.nlm.nih.gov/36288724/
2. Moorman, Hannah R, Reategui, Yazmin, Poschel, Dakota B, Liu, Kebin. 2022. IRF8: Mechanism of Action and Health Implications. In Cells, 11, . doi:10.3390/cells11172630. https://pubmed.ncbi.nlm.nih.gov/36078039/
3. Liang, Kai Ling, Laurenti, Elisa, Taghon, Tom. 2023. Circulating IRF8-expressing CD123+CD127+ lymphoid progenitors: key players in human hematopoiesis. In Trends in immunology, 44, 678-692. doi:10.1016/j.it.2023.07.004. https://pubmed.ncbi.nlm.nih.gov/37591714/
4. Saeki, Keita, Pan, Richard, Lee, Eunju, Kurotaki, Daisuke, Ozato, Keiko. 2024. IRF8 defines the epigenetic landscape in postnatal microglia, thereby directing their transcriptome programs. In Nature immunology, 25, 1928-1942. doi:10.1038/s41590-024-01962-2. https://pubmed.ncbi.nlm.nih.gov/39313544/
5. Salem, Sandra, Salem, David, Gros, Philippe. 2020. Role of IRF8 in immune cells functions, protection against infections, and susceptibility to inflammatory diseases. In Human genetics, 139, 707-721. doi:10.1007/s00439-020-02154-2. https://pubmed.ncbi.nlm.nih.gov/32232558/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
