C57BL/6NCya-Cavin1em1/Cya
Common Name:
Cavin1-KO
Product ID:
S-KO-03943
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cavin1-KO
Strain ID
KOCMP-19285-Cavin1-B6N-VA
Gene Name
Product ID
S-KO-03943
Gene Alias
2310075E07Rik; Cav-p60; Cavin; Ptrf
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Cavin1em1/Cya mice (Catalog S-KO-03943) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000060792
NCBI RefSeq
NM_008986
Target Region
Exon 1~2
Size of Effective Region
~11.8 kb
Detailed Document
Overview of Gene Research
Cavin1, also known as caveolae-associated protein 1, is essential for caveolae biogenesis, working together with caveolin 1. Caveolae are involved in multiple cellular processes such as control of membrane tension, signaling cascades, and lipid sorting [3,4,5]. Cavin1 may also be involved in various pathways related to lipid metabolism, cell-cell communication, and signal transduction. Understanding Cavin1's function can be facilitated through genetic models like gene knockout (KO) or conditional knockout (CKO) mouse models.
In the context of drug-induced QT prolongation (diLQT), Cavin1 expression in patient-specific induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) affects the sensitivity to hERG blockers. Higher Cavin1 expression in more sensitive iPS-CMs leads to a rapid translocation of hERG channels from the membrane to cytoskeleton-associated fractions upon sotalol application, reducing the delayed-rectifier potassium current (IKr) [1]. In glioblastoma, EPIC-1042, a small-molecular inhibitor, can interrupt the interaction between PTRF/Cavin1 and caveolin-1, reducing temozolomide (TMZ) efflux and promoting PARP1 degradation via autolysosomes [2]. In congenital generalized lipodystrophy type 4 (CGL4), a novel pathogenic mutation in the CAVIN1/PTRF gene was found in two siblings, resulting in a phenotype characterized by lack of adipose tissue, muscular dystrophy, and other symptoms [3]. Cavin1 deficiency in C57BL/6J mice causes neonatal death due to disorder of hepatic glycogen metabolism and impaired fenestration in liver sinusoidal endothelial cells (LSECs) [6].
In summary, Cavin1 plays crucial roles in caveolae biogenesis, which impacts diverse biological processes. Its study through KO/CKO mouse models has provided insights into diseases such as diLQT, glioblastoma, CGL4, and neonatal metabolic disorders related to hepatic function. These findings contribute to a better understanding of the underlying mechanisms of these diseases and may potentially lead to new therapeutic strategies.
References:
1. Al Sayed, Zeina R, Pereira, Céline, Le Borgne, Rémi, Trégouët, David-Alexandre, Hulot, Jean-Sébastien. 2024. CAVIN1-Mediated hERG Dynamics: A Novel Mechanism Underlying the Interindividual Variability in Drug-Induced Long QT. In Circulation, 150, 563-576. doi:10.1161/CIRCULATIONAHA.123.063917. https://pubmed.ncbi.nlm.nih.gov/38682330/
2. Hong, Biao, Yang, Eryan, Su, Dongyuan, Cui, Longtao, Kang, Chunsheng. . EPIC-1042 as a potent PTRF/Cavin1-caveolin-1 interaction inhibitor to induce PARP1 autophagic degradation and suppress temozolomide efflux for glioblastoma. In Neuro-oncology, 26, 100-114. doi:10.1093/neuonc/noad159. https://pubmed.ncbi.nlm.nih.gov/37651725/
3. Mancioppi, Valentina, Daffara, Tommaso, Romanisio, Martina, Giordano, Mara, Prodam, Flavia. 2023. A new mutation in the CAVIN1/PTRF gene in two siblings with congenital generalized lipodystrophy type 4: case reports and review of the literature. In Frontiers in endocrinology, 14, 1212729. doi:10.3389/fendo.2023.1212729. https://pubmed.ncbi.nlm.nih.gov/37501786/
4. Liu, Kang-Cheng, Pace, Hudson, Larsson, Elin, Hubert, Madlen, Lundmark, Richard. 2022. Membrane insertion mechanism of the caveola coat protein Cavin1. In Proceedings of the National Academy of Sciences of the United States of America, 119, e2202295119. doi:10.1073/pnas.2202295119. https://pubmed.ncbi.nlm.nih.gov/35696574/
5. Tillu, Vikas A, Rae, James, Gao, Ya, Parton, Robert G, Collins, Brett M. 2021. Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation. In Nature communications, 12, 931. doi:10.1038/s41467-021-21035-4. https://pubmed.ncbi.nlm.nih.gov/33568658/
6. Wei, Zhuang, Lei, Jigang, Shen, Feng, Liao, Kan, Hong, Shangyu. 2020. Cavin1 Deficiency Causes Disorder of Hepatic Glycogen Metabolism and Neonatal Death by Impacting Fenestrations in Liver Sinusoidal Endothelial Cells. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7, 2000963. doi:10.1002/advs.202000963. https://pubmed.ncbi.nlm.nih.gov/33042738/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen