C57BL/6JCya-Sdf2em1/Cya
Common Name:
Sdf2-KO
Product ID:
S-KO-04243
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Sdf2-KO
Strain ID
KOCMP-20316-Sdf2-B6J-VA
Gene Name
Product ID
S-KO-04243
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sdf2em1/Cya mice (Catalog S-KO-04243) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000002133
NCBI RefSeq
NM_009143
Target Region
Exon 2
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Sdf2, or stromal cell-derived factor 2, is an endoplasmic reticulum (ER)-resident protein [2]. It belongs to the stromal derived factor (SDFs) family. Sdf2 is involved in multiple biological processes such as the unfolded protein response (UPR) pathway when the ER is stressed due to misfolded protein accumulation [1,2,4,7]. It is also associated with protein quality control in the ER [5].
In rice, silencing of Sdf2 genes in the XA21 rice genetic background compromises resistance to Xanthomonas oryzae pv. oryzae (Xoo), indicating its importance in XA21-mediated immunity [3]. In endothelial cells, Sdf2 knockdown impairs agonist-stimulated NO synthesis and decreases the phosphorylation of eNOS at Ser(1177), while overexpression enhances NO synthesis, suggesting its role in Hsp90-dependent eNOS activation [6].
In conclusion, Sdf2 is crucial for processes like the UPR, protein quality control, plant immunity, and endothelial cell signal transduction. Its study in different models helps understand its role in various biological functions and disease-related processes, such as potential implications in pre-eclampsia which is associated with ER stress [1].
References:
1. Lorenzon-Ojea, Aline R, Yung, Hong Wa, Burton, Graham J, Bevilacqua, Estela. 2019. The potential contribution of stromal cell-derived factor 2 (SDF2) in endoplasmic reticulum stress response in severe preeclampsia and labor-onset. In Biochimica et biophysica acta. Molecular basis of disease, 1866, 165386. doi:10.1016/j.bbadis.2019.01.012. https://pubmed.ncbi.nlm.nih.gov/30776414/
2. Lorenzon-Ojea, Aline R, Caldeira, Waldir, Ribeiro, Alberto F, Guzzo, Cristiane R, Bevilacqua, Estela. 2014. Stromal cell derived factor-2 (Sdf2): a novel protein expressed in mouse. In The international journal of biochemistry & cell biology, 53, 262-70. doi:10.1016/j.biocel.2014.05.024. https://pubmed.ncbi.nlm.nih.gov/24878610/
3. Park, Chang-Jin, Sharma, Rita, Lefebvre, Benoit, Canlas, Patrick E, Ronald, Pamela C. 2013. The endoplasmic reticulum-quality control component SDF2 is essential for XA21-mediated immunity in rice. In Plant science : an international journal of experimental plant biology, 210, 53-60. doi:10.1016/j.plantsci.2013.05.003. https://pubmed.ncbi.nlm.nih.gov/23849113/
4. Schott, Andrea, Ravaud, Stéphanie, Keller, Sabrina, Sinning, Irmgard, Strahl, Sabine. 2010. Arabidopsis stromal-derived Factor2 (SDF2) is a crucial target of the unfolded protein response in the endoplasmic reticulum. In The Journal of biological chemistry, 285, 18113-21. doi:10.1074/jbc.M110.117176. https://pubmed.ncbi.nlm.nih.gov/20378538/
5. Fujimori, Tsutomu, Suno, Ryoji, Iemura, Shun-Ichiro, Wada, Ikuo, Hosokawa, Nobuko. 2017. Endoplasmic reticulum proteins SDF2 and SDF2L1 act as components of the BiP chaperone cycle to prevent protein aggregation. In Genes to cells : devoted to molecular & cellular mechanisms, 22, 684-698. doi:10.1111/gtc.12506. https://pubmed.ncbi.nlm.nih.gov/28597544/
6. Siragusa, Mauro, Fröhlich, Florian, Park, Eon Joo, Walther, Tobias C, Sessa, William C. 2015. Stromal cell-derived factor 2 is critical for Hsp90-dependent eNOS activation. In Science signaling, 8, ra81. doi:10.1126/scisignal.aaa2819. https://pubmed.ncbi.nlm.nih.gov/26286023/
7. Schott, Andrea, Strahl, Sabine. . Methods to study stromal-cell derived factor 2 in the context of ER stress and the unfolded protein response in Arabidopsis thaliana. In Methods in enzymology, 490, 295-319. doi:10.1016/B978-0-12-385114-7.00017-9. https://pubmed.ncbi.nlm.nih.gov/21266257/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen