C57BL/6JCya-Srcem1/Cya
Common Name
Src-KO
Product ID
S-KO-04501
Backgroud
C57BL/6JCya
Strain ID
KOCMP-20779-Src-B6J-VA
When using this mouse strain in a publication, please cite “Src-KO Mouse (Catalog S-KO-04501) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Src-KO
Strain ID
KOCMP-20779-Src-B6J-VA
Gene Name
Product ID
S-KO-04501
Gene Alias
pp60c-src
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000029175
NCBI RefSeq
NM_001025395
Target Region
Exon 5
Size of Effective Region
~0.1 kb
Overview of Gene Research
Src, the proto-oncogene tyrosine-protein kinase, is a key protein-tyrosine kinase. It participates in cell growth, division, migration, and survival signaling pathways. From N-to C-terminus, it contains a unique domain, SH3, SH2, a protein-tyrosine kinase domain, and a regulatory tail. It activates downstream signaling pathways like PI3K-AKT, Ras-MAPK, JAK-STAT3, and FAK/Paxillin, crucial for multiple cellular processes [1,2].
Src-knockout mice have provided significant insights. In bone, Src-knockout mice exhibit osteopetrosis as Src is essential for osteoclast-mediated bone resorption and suppresses osteoblast-mediated bone formation [3]. In platelets, studies using SRC knockout mice showed that SRC mediates platelet spreading on fibrinogen, while an activating pathological SRC missense variant in humans affects platelet activation and spreading [4].
In conclusion, Src is vital for numerous biological functions including those in cell growth, bone homeostasis, and platelet function. Gene-knockout mouse models, such as Src-knockout mice, have been instrumental in revealing Src's role in bone-related diseases like osteoporosis and in understanding platelet-related disorders, providing a foundation for potential therapeutic strategies targeting Src in these disease areas.
References:
1. Roskoski, Robert. 2015. Src protein-tyrosine kinase structure, mechanism, and small molecule inhibitors. In Pharmacological research, 94, 9-25. doi:10.1016/j.phrs.2015.01.003. https://pubmed.ncbi.nlm.nih.gov/25662515/
2. Raji, Lukmon, Tetteh, Angelina, Amin, A R M Ruhul. 2023. Role of c-Src in Carcinogenesis and Drug Resistance. In Cancers, 16, . doi:10.3390/cancers16010032. https://pubmed.ncbi.nlm.nih.gov/38201459/
3. Matsubara, Takuma, Yasuda, Kazuma, Mizuta, Kana, Kawaue, Hiroka, Kokabu, Shoichiro. 2022. Tyrosine Kinase Src Is a Regulatory Factor of Bone Homeostasis. In International journal of molecular sciences, 23, . doi:10.3390/ijms23105508. https://pubmed.ncbi.nlm.nih.gov/35628319/
4. De Kock, Lore, Freson, Kathleen. 2020. The (Patho)Biology of SRC Kinase in Platelets and Megakaryocytes. In Medicina (Kaunas, Lithuania), 56, . doi:10.3390/medicina56120633. https://pubmed.ncbi.nlm.nih.gov/33255186/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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