C57BL/6NCya-Suv39h1em1/Cya
Common Name:
Suv39h1-KO
Product ID:
S-KO-04662
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Suv39h1-KO
Strain ID
KOCMP-20937-Suv39h1-B6N-VA
Gene Name
Product ID
S-KO-04662
Gene Alias
DXHXS7466e; H3-K9-HMTase 1; KMT1A; mIS6
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Suv39h1em1/Cya mice (Catalog S-KO-04662) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000115638
NCBI RefSeq
NM_011514
Target Region
Exon 2~3
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Suv39h1, a lysine methyltransferase, is known for its role in introducing di-and trimethylation at histone H3 lysine 9 (H3K9). It is crucial in maintaining heterochromatin and gene repression, and is involved in various biological processes [6]. Genetic models, such as KO and CKO mouse models, have been valuable in studying its functions.
In liver fibrosis, HSC-specific or myofibroblast-specific deletion of Suv39h1 in mice ameliorated liver fibrosis. Suv39h1 inhibition by chaetocin also mitigated liver fibrosis in mice. Mechanistically, Suv39h1 bound to the promoter of heme oxygenase 1 (HMOX1) and repressed its transcription [1]. In adoptive T-cell therapies, genetic disruption of SUV39H1 enhanced the early expansion, long-term persistence, and overall antitumor efficacy of human CAR T cells in leukemia and prostate cancer models [2]. Also, in solid tumor models, inactivation of SUV39H1 enhanced BBz-CAR T cell long-term persistence, protecting mice against tumor relapses and rechallenges [3]. In limb ischemia of mice, pharmaceutical inhibition or genetic deletion of SUV39H1 significantly improved blood perfusion, capillary density, and angiogenesis in ischemic muscle tissue [4]. In liver ischemia-reperfusion injury, global or hepatocyte conditional Suv39h1 KO mice were protected from liver I/R injury, and a small-molecule SUV39H1 inhibitor achieved similar hepatoprotective effects [5]. In MLL-AF9-induced AML, Suv39h1 overexpression increased leukemia latency and decreased the frequency of LSCs, while Suv39h1 knockdown accelerated disease progression [7]. In glioblastoma, knockdown of SUV39H1 in patient-derived GSCs impaired their proliferation and stemness, and treatment with a SUV39H1 inhibitor sensitized GSCs to temozolomide [8].
In conclusion, Suv39h1 plays essential roles in multiple biological processes and diseases. Model-based research, especially through Suv39h1 KO/CKO mouse models, has revealed its significance in liver fibrosis, adoptive T-cell therapies for cancer, limb ischemia, liver ischemia-reperfusion injury, AML, and glioblastoma. Understanding Suv39h1 functions provides potential therapeutic targets for these disease areas.
References:
1. Kong, Ming, Zhou, Junjing, Kang, Aoqi, Xu, Yong, Li, Zilong. 2024. Histone methyltransferase Suv39h1 regulates hepatic stellate cell activation and is targetable in liver fibrosis. In Gut, 73, 810-824. doi:10.1136/gutjnl-2023-329671. https://pubmed.ncbi.nlm.nih.gov/38176898/
2. Jain, Nayan, Zhao, Zeguo, Koche, Richard P, Giavridis, Theodoros, Sadelain, Michel. . Disruption of SUV39H1-Mediated H3K9 Methylation Sustains CAR T-cell Function. In Cancer discovery, 14, 142-157. doi:10.1158/2159-8290.CD-22-1319. https://pubmed.ncbi.nlm.nih.gov/37934007/
3. López-Cobo, Sheila, Fuentealba, Jaime R, Gueguen, Paul, Saitakis, Michael, Amigorena, Sebastian. . SUV39H1 Ablation Enhances Long-term CAR T Function in Solid Tumors. In Cancer discovery, 14, 120-141. doi:10.1158/2159-8290.CD-22-1350. https://pubmed.ncbi.nlm.nih.gov/37934001/
4. Niu, Wenhao, Cao, Wenyue, Wu, Feng, Liang, Chun. . SUV39H1 Inhibits Angiogenesis in Limb Ischemia of Mice. In Cell transplantation, 32, 9636897231198167. doi:10.1177/09636897231198167. https://pubmed.ncbi.nlm.nih.gov/37811706/
5. Li, Zilong, Li, Jichen, Wu, Meng, Qin, Lei, Fan, Zhiwen. 2024. Redox-sensitive epigenetic activation of SUV39H1 contributes to liver ischemia-reperfusion injury. In Redox biology, 78, 103414. doi:10.1016/j.redox.2024.103414. https://pubmed.ncbi.nlm.nih.gov/39603205/
6. Weirich, Sara, Khella, Mina S, Jeltsch, Albert. 2021. Structure, Activity and Function of the Suv39h1 and Suv39h2 Protein Lysine Methyltransferases. In Life (Basel, Switzerland), 11, . doi:10.3390/life11070703. https://pubmed.ncbi.nlm.nih.gov/34357075/
7. Chu, Yajing, Chen, Yangpeng, Guo, Huidong, Shi, Jun, Yuan, Weiping. 2020. SUV39H1 regulates the progression of MLL-AF9-induced acute myeloid leukemia. In Oncogene, 39, 7239-7252. doi:10.1038/s41388-020-01495-6. https://pubmed.ncbi.nlm.nih.gov/33037410/
8. Li, Chunying, Xie, Qiqi, Ghosh, Sugata, Nephew, Kenneth P, Shen, Jia. 2024. SUV39H1 Preserves Cancer Stem Cell Chromatin State and Properties in Glioblastoma. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.08.15.607856. https://pubmed.ncbi.nlm.nih.gov/39229036/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen