C57BL/6JCya-Scapem1/Cya
Common Name
Scap-KO
Product ID
S-KO-06831
Backgroud
C57BL/6JCya
Strain ID
KOCMP-235623-Scap-B6J-VB
When using this mouse strain in a publication, please cite “Scap-KO Mouse (Catalog S-KO-06831) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Scap-KO
Strain ID
KOCMP-235623-Scap-B6J-VB
Gene Name
Product ID
S-KO-06831
Gene Alias
9530044G19, mKIAA0199
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 9
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000098350
NCBI RefSeq
NM_001001144.3
Target Region
Exon 3
Size of Effective Region
~1.3 kb
Overview of Gene Research
Scap, short for SREBP cleavage-activating protein, is a polytopic membrane protein. It functions as a key regulator in controlling the cholesterol content of membranes in mammalian cells. Scap binds to sterol regulatory element-binding proteins (SREBPs) and transports them from the endoplasmic reticulum (ER) to the Golgi for proteolytic processing. This process is integral to cholesterol synthesis and uptake regulation, and thus crucial for maintaining cholesterol homeostasis [1].
In murine models, deletion of Scap in the liver, a form of loss-of-function experiment, exacerbated liver injury, fibrosis, and carcinogenesis in non-alcoholic steatohepatitis (NASH), despite reducing hepatic steatosis. This was due to altered fatty acid composition of phosphatidylcholines, endoplasmic reticulum stress, and hepatocyte injury [2]. In macrophages, Scap-SREBP2 complex translocation is required for optimal NLRP3 inflammasome activation, showing its role in integrating cholesterol metabolism with inflammation [3].
In conclusion, Scap is essential for regulating cholesterol homeostasis, lipid metabolism, and inflammation. Studies using Scap KO/CKO mouse models have provided insights into its role in diseases like NASH and in immune-metabolic crosstalk, highlighting its potential as a therapeutic target for related metabolic and inflammatory diseases.
References:
1. Brown, Michael S, Radhakrishnan, Arun, Goldstein, Joseph L. 2017. Retrospective on Cholesterol Homeostasis: The Central Role of Scap. In Annual review of biochemistry, 87, 783-807. doi:10.1146/annurev-biochem-062917-011852. https://pubmed.ncbi.nlm.nih.gov/28841344/
2. Kawamura, Satoshi, Matsushita, Yuki, Kurosaki, Shigeyuki, Koike, Kazuhiko, Nakagawa, Hayato. . Inhibiting SCAP/SREBP exacerbates liver injury and carcinogenesis in murine nonalcoholic steatohepatitis. In The Journal of clinical investigation, 132, . doi:10.1172/JCI151895. https://pubmed.ncbi.nlm.nih.gov/35380992/
3. Guo, Chuansheng, Chi, Zhexu, Jiang, Danlu, Song, Bao-Liang, Wang, Di. 2018. Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. In Immunity, 49, 842-856.e7. doi:10.1016/j.immuni.2018.08.021. https://pubmed.ncbi.nlm.nih.gov/30366764/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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