Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Fbh1em1/Cya
Common Name:
Fbh1-KO
Product ID:
S-KO-10207
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Fbh1-KO
Strain ID
KOCMP-50755-Fbh1-B6J-VB
Gene Name
Fbh1
Product ID
S-KO-10207
Gene Alias
Fbx18; Fbxo18
Background
C57BL/6JCya
NCBI ID
50755
Modification
Conventional knockout
Chromosome
2
Phenotype
MGI:1354699
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fbh1em1/Cya mice (Catalog S-KO-10207) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000071564
NCBI RefSeq
NM_015792
Target Region
Exon 3~15
Size of Effective Region
~17.8 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Fbh1, or F-box DNA helicase 1, is a member of the UvrD family of DNA helicases. It plays crucial roles in the DNA damage response, especially in the regulation of cell responses to replicative stress [2,4,6]. FBH1 is involved in pathways related to DNA replication fork processing, homologous recombination, and cell cycle checkpoint control, which are essential for maintaining genome integrity [2,3,4,7].

FBH1-deficient cells show a reduction in ssDNA and double-strand break signaling when treated with WEE1 inhibitors, indicating its requirement for the induction of replication stress response [1,5]. However, FBH1-deficiency sensitizes cells to WEE1 inhibition by increasing mitotic catastrophe, suggesting that loss of FBH1 leads to replication-associated damage that needs the WEE1-dependent G2 checkpoint for repair [1,5]. Also, FBH1 depletion promotes UV-mediated transformation of human melanocytes, and its inactivation may contribute to oncogenic transformation [6].

In conclusion, FBH1 is essential for regulating the cell's response to replication stress, maintaining genome integrity, and preventing oncogenic transformation. Studies on FBH1-deficient cells have revealed its role in replication-associated damage repair and cell cycle checkpoint control, which are relevant to cancer therapy, especially in the context of WEE1 inhibition [1,5,6].

References:

1. Jennings, Lucy, Walters, Heather Andrews, Mason, Jennifer M. 2023. FBH1 deficiency sensitizes cells to WEE1 inhibition by promoting mitotic catastrophe. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.05.15.540841. https://pubmed.ncbi.nlm.nih.gov/37292855/

2. Liu, Jiuyang, Chaves-Arquero, Belén, Wei, Pengcheng, Blanco, Francisco J, Kutateladze, Tatiana G. 2023. Molecular insight into the PCNA-binding mode of FBH1. In Structure (London, England : 1993), 31, 511-517.e3. doi:10.1016/j.str.2023.03.004. https://pubmed.ncbi.nlm.nih.gov/36990095/

3. Liu, Guangxue, Li, Jimin, He, Boxue, Zhang, Xinghua, Chen, Xuefeng. 2023. Bre1/RNF20 promotes Rad51-mediated strand exchange and antagonizes the Srs2/FBH1 helicases. In Nature communications, 14, 3024. doi:10.1038/s41467-023-38617-z. https://pubmed.ncbi.nlm.nih.gov/37230987/

4. Hawks, Alexandra L, Bergmann, Amy, McCraw, Tyler J, Mason, Jennifer M. 2023. UBC13-mediated template switching promotes replication stress resistance in FBH1-deficient cells. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.09.04.556280. https://pubmed.ncbi.nlm.nih.gov/37732269/

5. Jennings, Lucy, Walters, Heather Andrews, McCraw, Tyler J, Turner, Joshua L, Mason, Jennifer M. 2023. FBH1 deficiency sensitizes cells to WEE1 inhibition by promoting mitotic catastrophe. In DNA repair, 133, 103611. doi:10.1016/j.dnarep.2023.103611. https://pubmed.ncbi.nlm.nih.gov/38103522/

6. Jeong, Yeon-Tae, Cermak, Lukas, Guijarro, Maria V, Hernando, Eva, Pagano, Michele. 2013. FBH1 protects melanocytes from transformation and is deregulated in melanomas. In Cell cycle (Georgetown, Tex.), 12, 1128-32. doi:10.4161/cc.24165. https://pubmed.ncbi.nlm.nih.gov/23466708/

7. Fugger, Kasper, Mistrik, Martin, Neelsen, Kai J, Hickson, Ian D, Sørensen, Claus Storgaard. 2015. FBH1 Catalyzes Regression of Stalled Replication Forks. In Cell reports, 10, 1749-1757. doi:10.1016/j.celrep.2015.02.028. https://pubmed.ncbi.nlm.nih.gov/25772361/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest