C57BL/6JCya-Asnsd1em1/Cya
Common Name:
Asnsd1-KO
Product ID:
S-KO-13330
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Asnsd1-KO
Strain ID
KOCMP-70396-Asnsd1-B6J-VA
Gene Name
Product ID
S-KO-13330
Gene Alias
2210409M21Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Asnsd1em1/Cya mice (Catalog S-KO-13330) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027264
NCBI RefSeq
NM_133728
Target Region
Exon 4~6
Size of Effective Region
~5.2 kb
Detailed Document
Overview of Gene Research
Asnsd1, or asparagine synthetase domain containing 1, is conserved across many species with maximal expression in skeletal muscle [1]. Its exact function remains somewhat elusive, but it likely plays a role in metabolic interactions between skeletal muscle and adipose tissue, potentially involved in pathways related to muscle maintenance and fat metabolism [1]. Genetic models, such as knockout mice, have been crucial for studying Asnsd1's function.
In Asnsd1 -/- mice, a progressive degenerative myopathy occurs, leading to severe sarcopenia and myosteatosis [1]. These mice exhibit severe muscle weakness and a significantly increased body fat percentage, even on normal chow and high-fat diets [1]. Histologically, muscle is replaced by well-differentiated adipose tissue with minimal inflammation, fibrosis, and muscle regeneration [1]. The lack of lipotoxicity despite high body fat and low muscle mass suggests metabolic dysfunctions contribute to this phenotype [1]. In addition, a microprotein encoded by ASNSD1-uORF (ASDURF) is upregulated in childhood medulloblastoma, associated with MYC-family oncogenes, and promotes cancer cell survival through interaction with the prefoldin-like chaperone complex [2,3,4].
In conclusion, Asnsd1 is important for maintaining normal muscle function and may be involved in regulating the balance between muscle and adipose tissue. The Asnsd1 -/- mouse model has provided insights into the role of this gene in myopathy and myosteatosis, as well as the discovery of its microprotein's function in medulloblastoma. These findings highlight the significance of Asnsd1 in understanding muscle-adipose metabolic interactions and cancer-related processes.
References:
1. Vogel, Peter, Ding, Zhi-Ming, Read, Robert, Brommage, Robert, Powell, David R. 2020. Progressive Degenerative Myopathy and Myosteatosis in ASNSD1-Deficient Mice. In Veterinary pathology, 57, 723-735. doi:10.1177/0300985820939251. https://pubmed.ncbi.nlm.nih.gov/32638637/
2. Hofman, Damon A, Ruiz-Orera, Jorge, Yannuzzi, Ian, van Heesch, Sebastiaan, Prensner, John R. 2024. Translation of non-canonical open reading frames as a cancer cell survival mechanism in childhood medulloblastoma. In Molecular cell, 84, 261-276.e18. doi:10.1016/j.molcel.2023.12.003. https://pubmed.ncbi.nlm.nih.gov/38176414/
3. Delaidelli, Alberto, Oliveira de Santis, Jessica, Sorensen, Poul H. . Actions speak louder than ORFs: A non-canonical microprotein promotes medulloblastoma oncogenesis. In Molecular cell, 84, 188-190. doi:10.1016/j.molcel.2023.12.027. https://pubmed.ncbi.nlm.nih.gov/38242097/
4. Hofman, Damon A, Ruiz-Orera, Jorge, Yannuzzi, Ian, van Heesch, Sebastiaan, Prensner, John R. 2023. Translation of non-canonical open reading frames as a cancer cell survival mechanism in childhood medulloblastoma. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.05.04.539399. https://pubmed.ncbi.nlm.nih.gov/37205492/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen