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C57BL/6JCya-Spats1em1/Cya
Common Name:
Spats1-KO
Product ID:
S-KO-13489
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Spats1-KO
Strain ID
KOCMP-71020-Spats1-B6J-VA
Gene Name
Spats1
Product ID
S-KO-13489
Gene Alias
1700011H05Rik; 4933400B06Rik; Ddip; Srsp1
Background
C57BL/6JCya
NCBI ID
71020
Modification
Conventional knockout
Chromosome
17
Phenotype
MGI:1918270
Document
Click here to download >>
Application
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More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Spats1em1/Cya mice (Catalog S-KO-13489) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024731
NCBI RefSeq
NM_027649
Target Region
Exon 2
Size of Effective Region
~0.8 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Spats1, also known as Dishevelled-DEP domain Interacting Protein (DDIP), is a testis-specific protein that has been implicated in spermatogenesis [3,5]. It contains a long serine stretch and a probable bipartite nuclear localization signal. Some reports suggest a link to human pathologies like male infertility and testicular cancer, and it may be involved in the Wnt signaling pathway [1,5]. Genetic models, such as gene knockout mouse models, are valuable for studying its function.

A Spats1 loss-of-function mouse model generated using Nuclease technology technology showed no overt phenotype, with both male and female mice being fertile. There were no differences in testicular content, histology, sperm concentration, motility, or morphology between wild-type and knockout mice. Also, no changes were detected in the expression levels of typical Wnt pathway-target genes in mutant individuals [1]. In the Chinese soft-shelled turtle, Spats1 is a male-specific expressed gene mainly regulated by 17α-methyltestosterone (MT) and is closely linked to spermatogenesis and release. Its mRNA is highly expressed in the testes and specifically expressed in germ cells [2]. In rats, Spats1 is first expressed in the embryo at 17.5 days post-coitum, and its expression is highest during meiosis of the first spermatogenic wave, suggesting a role in the initiation of the first spermatogenic wave and the first male meiotic division [3]. Bioinformatics analysis identified Spats1 as a hub gene for spermatogenesis, and its expression was examined in testicular germ cell tumors and lipopolysaccharide-induced acute orchitis mice [4].

In conclusion, while Spats1 alteration might be a risk factor for male testicular health, studies using gene knockout mouse models have shown that this gene is not individually essential for male fertility and spermatogenesis in mice. Its role in spermatogenesis and potential association with male-related pathologies in other species, as seen in turtles, rats, and through bioinformatics analysis in humans, indicate its importance in understanding testicular biology and male-related diseases.

References:

1. Capoano, Carlos A, Ortiz-Laquintana, Luis Adrián, Rodríguez-Casuriaga, Rosana, Benavente, Ricardo, Geisinger, Adriana. 2021. SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse. In PloS one, 16, e0251028. doi:10.1371/journal.pone.0251028. https://pubmed.ncbi.nlm.nih.gov/33945571/

2. Lei, Luo, Zhu, Junxian, Chen, Chen, Li, Wei, Zhu, Xinping. 2022. Expression and Characterization of the Spats1 Gene and Its Response to E2/MT Treatment in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis). In Animals : an open access journal from MDPI, 12, . doi:10.3390/ani12141858. https://pubmed.ncbi.nlm.nih.gov/35883403/

3. Capoano, Carlos A, Wettstein, Rodolfo, Kun, Alejandra, Geisinger, Adriana. 2009. Spats 1 (Srsp1) is differentially expressed during testis development of the rat. In Gene expression patterns : GEP, 10, 1-8. doi:10.1016/j.gep.2009.11.006. https://pubmed.ncbi.nlm.nih.gov/19948251/

4. Liu, Shuang, Bian, Yan-Chao, Wang, Wan-Lun, Xiao, Rui, Zhang, Chuan-Ling. 2023. Identification of hub genes associated with spermatogenesis by bioinformatics analysis. In Scientific reports, 13, 18435. doi:10.1038/s41598-023-45620-3. https://pubmed.ncbi.nlm.nih.gov/37891374/

5. Zhang, Haiwei, Zhang, Hui, Zhang, Yanquan, Guo, Qinglong, Chang, Zhijie. 2010. Dishevelled-DEP domain interacting protein (DDIP) inhibits Wnt signaling by promoting TCF4 degradation and disrupting the TCF4/beta-catenin complex. In Cellular signalling, 22, 1753-60. doi:10.1016/j.cellsig.2010.06.016. https://pubmed.ncbi.nlm.nih.gov/20603214/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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