C57BL/6JCya-Apobec3em1/Cya
Common Name
Apobec3-KO
Product ID
S-KO-15286
Backgroud
C57BL/6JCya
Strain ID
KOCMP-80287-Apobec3-B6J-VA
When using this mouse strain in a publication, please cite “Apobec3-KO Mouse (Catalog S-KO-15286) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Apobec3-KO
Strain ID
KOCMP-80287-Apobec3-B6J-VA
Gene Name
Product ID
S-KO-15286
Gene Alias
Apobec, Arp3, Cem15, Gm20117, Rfv3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 15
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000109620
NCBI RefSeq
NM_001160415
Target Region
Exon 3~9
Size of Effective Region
~10.7 kb
Overview of Gene Research
Apobec3, short for apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3, is a family of cytosine deaminases. These enzymes play crucial roles in the innate immune system, capable of inhibiting a wide range of endogenous and exogenous viruses via deaminase-dependent and-independent mechanisms [2]. They are also involved in restricting the mobilization of retrotransposons in primates [3].
In human cancer cells, deletion experiments in cell lines (functionally similar to gene knockout in models) have provided insights. Deletion of APOBEC3A diminished APOBEC3-associated mutational signatures, and deletion of both APOBEC3A and APOBEC3B further decreased APOBEC3 mutation burdens but did not eliminate them. In some cell lines, deletion of APOBEC3B increased APOBEC3A protein levels, activity, and APOBEC3A-mediated mutagenesis. These suggest APOBEC3A is a main driver of APOBEC3-related mutations in cancer, and APOBEC3B can both contribute to and restrain mutagenesis [1].
In summary, Apobec3 is essential for antiviral defense and retrotransposon restriction. Model-based research, like the gene-deletion experiments in cancer cell lines, reveals its complex role in cancer mutagenesis. Understanding Apobec3's functions helps in comprehending viral-host interactions and cancer evolution, potentially offering new strategies for treating virus-related diseases and cancers associated with APOBEC3-mediated mutagenesis.
References:
1. Petljak, Mia, Dananberg, Alexandra, Chu, Kevan, Stratton, Michael R, Maciejowski, John. 2022. Mechanisms of APOBEC3 mutagenesis in human cancer cells. In Nature, 607, 799-807. doi:10.1038/s41586-022-04972-y. https://pubmed.ncbi.nlm.nih.gov/35859169/
2. Sadeghpour, Shiva, Khodaee, Saeideh, Rahnama, Mostafa, Rahimi, Hamzeh, Ebrahimi, Diako. 2021. Human APOBEC3 Variations and Viral Infection. In Viruses, 13, . doi:10.3390/v13071366. https://pubmed.ncbi.nlm.nih.gov/34372572/
3. Modenini, Giorgia, Abondio, Paolo, Boattini, Alessio. 2022. The coevolution between APOBEC3 and retrotransposons in primates. In Mobile DNA, 13, 27. doi:10.1186/s13100-022-00283-1. https://pubmed.ncbi.nlm.nih.gov/36443831/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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