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C57BL/6NCya-Mphosph8em1/Cya
Common Name:
Mphosph8-KO
Product ID:
S-KO-15737
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mphosph8-KO
Strain ID
KOCMP-75339-Mphosph8-B6N-VB
Gene Name
Mphosph8
Product ID
S-KO-15737
Gene Alias
1500035L22Rik; 4930548G07Rik
Background
C57BL/6NCya
NCBI ID
75339
Modification
Conventional knockout
Chromosome
14
Phenotype
MGI:1922589
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Mphosph8em1/Cya mice (Catalog S-KO-15737) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000116468
NCBI RefSeq
NM_023773
Target Region
Exon 2~5
Size of Effective Region
~8.1 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Mphosph8, also known as MPP8 (M-phase phosphoprotein 8), is a component of the human silencing hub (HUSH) complex. It plays a crucial role in epigenetic regulation, particularly in the silencing of long interspersed element-1 (LINE-1 or L1) retrotransposons [1,2,4]. This silencing function is associated with maintaining genome stability and is involved in various biological processes. Genetic models, such as gene knockout mouse models, can be valuable for further exploring its functions.

In acute myeloid leukemia (AML), MPP8 is an AML-selective dependency. Loss of MPP8 inhibits AML development by reactivating L1s, which in turn induces the DNA damage response and cell cycle exit [1]. In medullary thyroid cancer (MTC), depletion of MPHOSPH8 significantly inhibits cell proliferation, leading to G0/G1 phase cell cycle arrest and apoptosis, suggesting it promotes cell proliferation in MTC [3]. In mice, genetic inactivation of Mphosph8 in the nervous system leads to increased brain size, altered brain architecture, and behavioral changes, as MPP8 suppresses the repetitive-like protocadherin gene cluster in an H3K9me3-dependent manner [5].

In summary, Mphosph8 is essential for the silencing of L1 retrotransposons through its role in the HUSH complex. Its function is critical in multiple disease areas, including AML, MTC, and in maintaining normal brain development. Studies using gene knockout mouse models have been instrumental in revealing these functions, deepening our understanding of the biological processes and disease mechanisms associated with Mphosph8.

References:

1. Gu, Zhimin, Liu, Yuxuan, Zhang, Yuannyu, Abrams, John M, Xu, Jian. 2021. Silencing of LINE-1 retrotransposons is a selective dependency of myeloid leukemia. In Nature genetics, 53, 672-682. doi:10.1038/s41588-021-00829-8. https://pubmed.ncbi.nlm.nih.gov/33833453/

2. Liu, Nian, Lee, Cameron H, Swigut, Tomek, Bassik, Michael C, Wysocka, Joanna. 2017. Selective silencing of euchromatic L1s revealed by genome-wide screens for L1 regulators. In Nature, 553, 228-232. doi:10.1038/nature25179. https://pubmed.ncbi.nlm.nih.gov/29211708/

3. Li, Peiyong, Yang, Weiping, Shen, Baiyong, Li, Hongwei, Yan, Jiqi. 2016. Lentivirus-mediated silencing of MPHOSPH8 inhibits MTC proliferation and enhances apoptosis. In Oncology letters, 11, 4117-4122. doi:. https://pubmed.ncbi.nlm.nih.gov/27313751/

4. Müller, Iris, Moroni, Ann Sophie, Shlyueva, Daria, Huang, Chang, Helin, Kristian. 2021. MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells. In Nature communications, 12, 3034. doi:10.1038/s41467-021-23308-4. https://pubmed.ncbi.nlm.nih.gov/34031396/

5. Hagelkruys, Astrid, Horrer, Marion, Taubenschmid-Stowers, Jasmin, Knoblich, Jürgen A, Penninger, Josef M. 2022. The HUSH complex controls brain architecture and protocadherin fidelity. In Science advances, 8, eabo7247. doi:10.1126/sciadv.abo7247. https://pubmed.ncbi.nlm.nih.gov/36332029/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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