C57BL/6JCya-Itgb5em1/Cya
Common Name:
Itgb5-KO
Product ID:
S-KO-16590
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Itgb5-KO
Strain ID
KOCMP-16419-Itgb5-B6J-VB
Gene Name
Product ID
S-KO-16590
Gene Alias
ESTM23; [b]-5; [b]5; [b]5A; [b]5B; beta-5; beta5
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Itgb5em1/Cya mice (Catalog S-KO-16590) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000069345
NCBI RefSeq
NM_010580
Target Region
Exon 3~10
Size of Effective Region
~56.3 kb
Detailed Document
Overview of Gene Research
Itgb5, encoding integrin β5 subunit, is a significant gene. Integrins are cell-adhesion molecules involved in various cellular processes such as cell-matrix adhesion, cell-cell interaction, and signal transduction. Itgb5, often assorting with the αv subunit to form integrin αvβ5, participates in multiple signaling pathways including integrin signaling, which is crucial for normal cell function, development, and tissue homeostasis [3].
In multiple diseases, Itgb5 shows distinct roles. In gastric cancer, knockdown of Itgb5 impairs cell metastasis both in vitro and in vivo. It acts as a scaffold to interact with TGFBR2 and SNX17, promoting TGFBR2 endosomal recycling, thus enhancing metastasis via a positive feedback loop with TGFβ signaling [1]. In pancreatic adenocarcinoma, knockdown of Itgb5 increases cell sensitivity to radiation by influencing DNA damage repair and the MEK/ERK signaling pathway [2]. In piglets, knockout of Itgb5 in IPEC-J2 cell lines using Nuclease technology significantly reduces the adhesion of ETEC F4ac to porcine intestinal epithelial cells, indicating its key role in susceptibility to ETEC F4ac-induced diarrhea [3]. In hepatocellular carcinoma, Itgb5 confers sorafenib resistance through a positive feedback loop involving EPS15, EGFR, and CSNK1A1 [4]. In colorectal cancer, silencing Itgb5 inhibits cell proliferation, invasion, and migration by blocking the Wnt/β-catenin signaling pathway and EMT [5].
In conclusion, Itgb5 plays essential roles in cell adhesion, metastasis, radiation resistance, and drug resistance through its involvement in multiple signaling pathways. Studies using gene knockout models in various disease conditions have revealed its significance in cancer metastasis, pathogen-induced diarrhea, and drug resistance, providing potential therapeutic targets for these diseases.
References:
1. Lei, Tianxiang, Lin, Yingying, Lai, Xiaofan, Feng, Wei, Song, Wu. 2024. ITGB5 facilitates gastric cancer metastasis by promoting TGFBR2 endosomal recycling. In Cancer letters, 592, 216953. doi:10.1016/j.canlet.2024.216953. https://pubmed.ncbi.nlm.nih.gov/38729557/
2. Wen, Xin, Chen, Si, Chen, Xueting, Ding, Xin, Zhang, Longzhen. 2022. ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway. In Frontiers in oncology, 12, 887068. doi:10.3389/fonc.2022.887068. https://pubmed.ncbi.nlm.nih.gov/36249018/
3. Wang, Wenwen, Liu, Yang, Tang, Hui, Yu, Ying, Zhang, Qin. 2019. ITGB5 Plays a Key Role in Escherichia coli F4ac-Induced Diarrhea in Piglets. In Frontiers in immunology, 10, 2834. doi:10.3389/fimmu.2019.02834. https://pubmed.ncbi.nlm.nih.gov/31921118/
4. Gu, Li, Jin, Xin, Liang, Huaiyuan, Yang, Chong, Zhang, Yu. 2023. Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex. In Pharmacological research, 192, 106789. doi:10.1016/j.phrs.2023.106789. https://pubmed.ncbi.nlm.nih.gov/37149115/
5. Chen, Zhihua, Fang, Yuan, Zhong, Shuwu, Yang, Xiaoyu, Chen, Shaoqin. 2025. ITGB5 is a prognostic factor in colorectal cancer and promotes cancer progression and metastasis through the Wnt signaling pathway. In Scientific reports, 15, 9225. doi:10.1038/s41598-025-93081-7. https://pubmed.ncbi.nlm.nih.gov/40097546/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen