Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Itgb5em1/Cya
Common Name:
Itgb5-KO
Product ID:
S-KO-16590
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Itgb5-KO
Strain ID
KOCMP-16419-Itgb5-B6J-VB
Gene Name
Itgb5
Product ID
S-KO-16590
Gene Alias
ESTM23; [b]-5; [b]5; [b]5A; [b]5B; beta-5; beta5
Background
C57BL/6JCya
NCBI ID
16419
Modification
Conventional knockout
Chromosome
16
Phenotype
MGI:96614
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Itgb5em1/Cya mice (Catalog S-KO-16590) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000069345
NCBI RefSeq
NM_010580
Target Region
Exon 3~10
Size of Effective Region
~56.3 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Itgb5, encoding integrin β5 subunit, is a significant gene. Integrins are cell-adhesion molecules involved in various cellular processes such as cell-matrix adhesion, cell-cell interaction, and signal transduction. Itgb5, often assorting with the αv subunit to form integrin αvβ5, participates in multiple signaling pathways including integrin signaling, which is crucial for normal cell function, development, and tissue homeostasis [3].

In multiple diseases, Itgb5 shows distinct roles. In gastric cancer, knockdown of Itgb5 impairs cell metastasis both in vitro and in vivo. It acts as a scaffold to interact with TGFBR2 and SNX17, promoting TGFBR2 endosomal recycling, thus enhancing metastasis via a positive feedback loop with TGFβ signaling [1]. In pancreatic adenocarcinoma, knockdown of Itgb5 increases cell sensitivity to radiation by influencing DNA damage repair and the MEK/ERK signaling pathway [2]. In piglets, knockout of Itgb5 in IPEC-J2 cell lines using Nuclease technology significantly reduces the adhesion of ETEC F4ac to porcine intestinal epithelial cells, indicating its key role in susceptibility to ETEC F4ac-induced diarrhea [3]. In hepatocellular carcinoma, Itgb5 confers sorafenib resistance through a positive feedback loop involving EPS15, EGFR, and CSNK1A1 [4]. In colorectal cancer, silencing Itgb5 inhibits cell proliferation, invasion, and migration by blocking the Wnt/β-catenin signaling pathway and EMT [5].

In conclusion, Itgb5 plays essential roles in cell adhesion, metastasis, radiation resistance, and drug resistance through its involvement in multiple signaling pathways. Studies using gene knockout models in various disease conditions have revealed its significance in cancer metastasis, pathogen-induced diarrhea, and drug resistance, providing potential therapeutic targets for these diseases.

References:

1. Lei, Tianxiang, Lin, Yingying, Lai, Xiaofan, Feng, Wei, Song, Wu. 2024. ITGB5 facilitates gastric cancer metastasis by promoting TGFBR2 endosomal recycling. In Cancer letters, 592, 216953. doi:10.1016/j.canlet.2024.216953. https://pubmed.ncbi.nlm.nih.gov/38729557/

2. Wen, Xin, Chen, Si, Chen, Xueting, Ding, Xin, Zhang, Longzhen. 2022. ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway. In Frontiers in oncology, 12, 887068. doi:10.3389/fonc.2022.887068. https://pubmed.ncbi.nlm.nih.gov/36249018/

3. Wang, Wenwen, Liu, Yang, Tang, Hui, Yu, Ying, Zhang, Qin. 2019. ITGB5 Plays a Key Role in Escherichia coli F4ac-Induced Diarrhea in Piglets. In Frontiers in immunology, 10, 2834. doi:10.3389/fimmu.2019.02834. https://pubmed.ncbi.nlm.nih.gov/31921118/

4. Gu, Li, Jin, Xin, Liang, Huaiyuan, Yang, Chong, Zhang, Yu. 2023. Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex. In Pharmacological research, 192, 106789. doi:10.1016/j.phrs.2023.106789. https://pubmed.ncbi.nlm.nih.gov/37149115/

5. Chen, Zhihua, Fang, Yuan, Zhong, Shuwu, Yang, Xiaoyu, Chen, Shaoqin. 2025. ITGB5 is a prognostic factor in colorectal cancer and promotes cancer progression and metastasis through the Wnt signaling pathway. In Scientific reports, 15, 9225. doi:10.1038/s41598-025-93081-7. https://pubmed.ncbi.nlm.nih.gov/40097546/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest