C57BL/6JCya-Stambpl1em1/Cya
Common Name:
Stambpl1-KO
Product ID:
S-KO-17327
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Stambpl1-KO
Strain ID
KOCMP-76630-Stambpl1-B6J-VB
Gene Name
Product ID
S-KO-17327
Gene Alias
1700095N21Rik; 8230401J17Rik; ALMalpha; AMSH-FP
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Stambpl1em1/Cya mice (Catalog S-KO-17327) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000054956
NCBI RefSeq
NM_029682.5
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
STAMBPL1, also known as STAM binding protein-like 1, is a Lys-63 linkage-specific deubiquitinase [2]. It is involved in multiple biological pathways. For example, it functions in concert with E3 ubiquitin ligase RNF167 to control the polyubiquitination level of Sestrin2 in response to leucine availability, which in turn modulates mTORC1 signaling [1]. It also participates in pathways related to the stability of proteins like EGFR, AXL, and MKP-1, as well as in the regulation of RNA splicing [2,4,5].
Knockout of STAMBPL1 in a human colon cancer cell line suppresses xenograft tumor growth, suggesting its role in promoting cancer progression [1]. In hepatocellular carcinoma, STAMBPL1 deficiency attenuates liver tumorigenesis both in vitro and in vivo [2]. In lung adenocarcinoma, knockdown of STAMBPL1 in A549 and H1299 cells suppresses cell growth, migration, invasiveness, and colony-forming ability while increasing apoptosis [3]. In breast cancer, depletion of STAMBPL1 sensitizes cells to cisplatin both in vitro and in vivo [5]. In gastric cancer, knockdown of STAMBPL1 significantly suppresses cell proliferation, increases apoptosis, and decreases invasion and migration [6].
In conclusion, STAMBPL1 plays crucial roles in multiple biological processes mainly through its deubiquitinase function. Studies using knockout models in various cancer cell lines have revealed its significant contribution to cancer progression in colon, liver, lung, breast, and gastric cancers, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Wang, Dong, Xu, Chenchen, Yang, Wenyu, Guan, Jialiang, Liu, Ying. 2022. E3 ligase RNF167 and deubiquitinase STAMBPL1 modulate mTOR and cancer progression. In Molecular cell, 82, 770-784.e9. doi:10.1016/j.molcel.2022.01.002. https://pubmed.ncbi.nlm.nih.gov/35114100/
2. Zhang, Hongli, Wang, Zixuan, Zhang, Jian, Chen, Wei-Dong, Wang, Yan-Dong. 2024. A MYC-STAMBPL1-TOE1 positive feedback loop mediates EGFR stability in hepatocellular carcinoma. In Cell reports, 43, 114812. doi:10.1016/j.celrep.2024.114812. https://pubmed.ncbi.nlm.nih.gov/39388352/
3. Yang, Xiang, Ling, Liqun, Li, Changhong, Wang, Yumin, Hu, Lijuan. 2023. STAMBPL1 promotes the progression of lung adenocarcinoma by inhibiting DHRS2 expression. In Translational oncology, 35, 101728. doi:10.1016/j.tranon.2023.101728. https://pubmed.ncbi.nlm.nih.gov/37393834/
4. Huang, Shiyu, Qin, Xuke, Fu, Shujie, Chen, Zhiyuan, Wang, Lei. 2024. STAMBPL1/TRIM21 Balances AXL Stability Impacting Mesenchymal Phenotype and Immune Response in KIRC. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 12, e2405083. doi:10.1002/advs.202405083. https://pubmed.ncbi.nlm.nih.gov/39527690/
5. Liu, Rong, Yang, Guangxi, Bao, Min, Huang, Jian, Chen, Ceshi. 2022. STAMBPL1 promotes breast cancer cell resistance to cisplatin partially by stabilizing MKP-1 expression. In Oncogene, 41, 2265-2274. doi:10.1038/s41388-022-02252-7. https://pubmed.ncbi.nlm.nih.gov/35236965/
6. Yu, Da-Jun, Qian, Jun, Jin, Xin, Guo, Chen-Xu, Yue, Xi-Cheng. 2019. STAMBPL1 knockdown has antitumour effects on gastric cancer biological activities. In Oncology letters, 18, 4421-4428. doi:10.3892/ol.2019.10789. https://pubmed.ncbi.nlm.nih.gov/31611951/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen