C57BL/6JCya-Abcc3em1/Cya
Common Name:
Abcc3-KO
Product ID:
S-KO-17500
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Abcc3-KO
Strain ID
KOCMP-76408-Abcc3-B6J-VB
Gene Name
Product ID
S-KO-17500
Gene Alias
1700019L09Rik; ABC31; MLP2; MOAT-D; MRP3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Abcc3em1/Cya mice (Catalog S-KO-17500) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000178136
NCBI RefSeq
NM_001363187
Target Region
Exon 9~11
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Abcc3, also known as MRP3, is a member of the ABCC subfamily of ATP - Binding Cassette (ABC) transporters. These transporters play a crucial role in the transport of endo - and xenobiotics, and Abcc3 specifically can confer resistance to natural, synthetic drugs and their conjugated metabolites [3]. It is involved in processes like bile acid secretion and may influence lysosomal homeostasis [2,4].
In hepatocellular carcinoma (HCC), BRG1 was found to directly bind to the Abcc3 promoter to activate its transcription, promoting HCC carcinogenesis. Depletion of BRG1 in BRG1high HCC cells decelerated proliferation and migration, effects that were rescued by Abcc3 over - expression [2]. In pancreatic ductal adenocarcinoma (PDAC), Abcc3 is overexpressed, and its disruption by genetic knockdown reduces pancreatic cancer cell growth in vitro and in vivo, through inhibition of STAT3 and HIF1α signalling pathways [5]. In colorectal cancer, down - regulation of Abcc3 activates MAPK signalling through the accumulation of deoxycholic acid [4].
In conclusion, Abcc3 is an important transporter gene involved in drug resistance and carcinogenesis in multiple cancer types. Studies using gene - knockdown or knockout models in these cancers have revealed its role in promoting cancer cell growth, proliferation, and migration, suggesting it could be a potential therapeutic target for improving cancer treatment [1,2,5].
References:
1. Ramírez-Cosmes, Adriana, Reyes-Jiménez, Edilburga, Zertuche-Martínez, Cecilia, Elizarrarás-Rivas, Jesús, Vásquez-Garzón, Verónica R. 2021. The implications of ABCC3 in cancer drug resistance: can we use it as a therapeutic target? In American journal of cancer research, 11, 4127-4140. doi:. https://pubmed.ncbi.nlm.nih.gov/34659880/
2. Liu, Huimin, Yue, Linbo, Hong, Wenxuan, Zhou, Junjing. 2024. SMARCA4 (BRG1) activates ABCC3 transcription to promote hepatocellular carcinogenesis. In Life sciences, 347, 122605. doi:10.1016/j.lfs.2024.122605. https://pubmed.ncbi.nlm.nih.gov/38642845/
3. Ghanem, Carolina I, Manautou, Jose E. . Modulation of Hepatic MRP3/ABCC3 by Xenobiotics and Pathophysiological Conditions: Role in Drug Pharmacokinetics. In Current medicinal chemistry, 26, 1185-1223. doi:10.2174/0929867325666180221142315. https://pubmed.ncbi.nlm.nih.gov/29473496/
4. Sato, Yukihiro, Kobayashi, Minoru, Ohira, Masahiro, Unno, Michiaki, Nakayama, Keiko. 2024. Downregulation of ABCC3 activates MAPK signaling through accumulation of deoxycholic acid in colorectal cancer cells. In Cancer science, 115, 1778-1790. doi:10.1111/cas.16132. https://pubmed.ncbi.nlm.nih.gov/38566304/
5. Adamska, Aleksandra, Ferro, Riccardo, Lattanzio, Rossano, Sala, Gianluca, Falasca, Marco. 2019. ABCC3 is a novel target for the treatment of pancreatic cancer. In Advances in biological regulation, 73, 100634. doi:10.1016/j.jbior.2019.04.004. https://pubmed.ncbi.nlm.nih.gov/31053501/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen