C57BL/6JCya-Stomem1/Cya
Common Name:
Stom-KO
Product ID:
S-KO-17695
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Stom-KO
Strain ID
KOCMP-13830-Stom-B6J-VB
Gene Name
Product ID
S-KO-17695
Gene Alias
Epb7.2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Stomem1/Cya mice (Catalog S-KO-17695) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028241
NCBI RefSeq
NM_013515
Target Region
Exon 2
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Stom, also known as stomatin, is a 31-kDa integral membrane protein. It belongs to the stomatin-domain protein family, which is ubiquitously present in all classes of life. Stom has been implicated in the regulation of ion transport, as in some cases of hereditary stomatocytosis where it is absent and red cells leak sodium and potassium ions [1]. Members of the stomatin-domain family are often oligomeric, localize to membrane domains, and modulate ion channel activity, suggesting Stom may have similar functions [2].
In a study on non-alcoholic fatty liver disease (NAFLD), it was found that lipid-induced DRAM recruits STOM to lysosomes and induces lysosomal membrane permeabilization (LMP) to promote exosome release from hepatocytes. Knockout or knockdown of DRAM in a mouse model and cell model respectively down-regulated exosome secretion, indicating STOM's role in this process [3]. In breast cancer, STOM is highly expressed in tumor-derived mesenchymal progenitor cells (MPCs). Overexpression of STOM in MPCs mediated their slow proliferation but promoted the proliferation of breast cancer cells, suggesting its significance in breast cancer-related microenvironment regulation [4]. In sepsis-induced acute lung injury, STOM expression was upregulated in lipopolysaccharide (LPS)-treated mouse lung epithelial cells (MLE-12). Knockdown of STOM reversed the inhibitory effects of LPS on cell viability and the promoting effects on oxidative stress and inflammation, and this was alleviated by overexpression of CD36, indicating STOM may interact with CD36 to affect these processes [5].
In conclusion, Stom plays diverse and important roles in biological processes such as ion transport, exosome secretion, and cell proliferation in relation to diseases like NAFLD, breast cancer, and sepsis-induced acute lung injury. Research using knockout or knockdown models in mice and cell lines has been crucial in uncovering these functions, providing insights into the underlying molecular mechanisms and potential therapeutic targets for these diseases.
References：
1. Stewart, G W. . Stomatin. In The international journal of biochemistry & cell biology, 29, 271-4. doi:. https://pubmed.ncbi.nlm.nih.gov/9147127/
2. Lapatsina, Liudmilla, Brand, Janko, Poole, Kate, Daumke, Oliver, Lewin, Gary R. 2011. Stomatin-domain proteins. In European journal of cell biology, 91, 240-5. doi:10.1016/j.ejcb.2011.01.018. https://pubmed.ncbi.nlm.nih.gov/21501885/
3. Zhang, Jie, Tan, Jie, Wang, Mengke, Xin, Yongning, Zhuang, Likun. 2021. Lipid-induced DRAM recruits STOM to lysosomes and induces LMP to promote exosome release from hepatocytes in NAFLD. In Science advances, 7, eabh1541. doi:10.1126/sciadv.abh1541. https://pubmed.ncbi.nlm.nih.gov/34731006/
4. Chen, Yizhu, Zhu, Li, Wang, Yiming, Xiao, Fengjun, Li, Xiru. 2024. Tumor-derived mesenchymal progenitor cell-related genes in the regulation of breast cancer proliferation. In Gland surgery, 13, 325-339. doi:10.21037/gs-23-387. https://pubmed.ncbi.nlm.nih.gov/38601284/
5. Wu, Kangkang, Wang, Li. 2021. Stomatin-knockdown effectively attenuates sepsis-induced oxidative stress and inflammation of alveolar epithelial cells by regulating CD36. In Experimental and therapeutic medicine, 23, 69. doi:10.3892/etm.2021.10992. https://pubmed.ncbi.nlm.nih.gov/34934440/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen