C57BL/6JCya-Camkk1em1/Cya
Common Name:
Camkk1-KO
Product ID:
S-KO-17708
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Camkk1-KO
Strain ID
KOCMP-55984-Camkk1-B6J-VB
Gene Name
Product ID
S-KO-17708
Gene Alias
CaMKKalpha; Camkk; caM-KK 1; caM-KK alpha; caMKK 1; caMKK alpha
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Camkk1em1/Cya mice (Catalog S-KO-17708) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000092937
NCBI RefSeq
NM_018883.3
Target Region
Exon 3~7
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Camkk1, also known as calcium/calmodulin-dependent protein kinase kinase 1, phosphorylates and enhances the catalytic activity of downstream kinases such as CaMKI, CaMKIV, and protein kinase B [4]. It is involved in regulating key physiological and pathological processes including tumorigenesis, neuronal morphogenesis, synaptic plasticity, transcription factor activation, and cellular energy homeostasis [4]. It also has a role in calcium signal transduction [3].
In non-alcoholic fatty liver disease (NAFLD), human umbilical cord mesenchymal stem cell-derived exosomes (MSC-ex) can transfer Camkk1, which ameliorates lipid accumulation in an AMP-activated protein kinase-dependent manner, inhibiting SREBP-1C-mediated fatty acid synthesis and enhancing peroxisome proliferator-activated receptor alpha (PPARα)-mediated fatty acid oxidation [1].
In lung cancer patients, the rs7214723 recessive CC genotype in the Camkk1 gene is associated with better overall survival, and the Nuclease technology-guided single nucleotide editing shows that the T > C mutation in rs7214723 significantly inhibits cell proliferation and migration and promotes cell apoptosis [2].
In a cross-sectional study on cardiac patients, the rs7214723 polymorphism in Camkk1 was analyzed, and although no association was found between it and the risk of developing specific coronary artery disease (CAD) and aortic stenosis (AS), the results suggest its potential role in CVD susceptibility as a possible genetic biomarker [3].
In the context of Echinococcus granulosus-induced bone loss, Camkk1 is up-regulated after Nrf2 suppression. Silencing Camkk1 inhibits osteoclast differentiation, and the small molecule inhibitor Crenolani can alleviate bone loss in PSC-treated mice [5].
In conclusion, Camkk1 is crucial in multiple biological processes and disease conditions. Its role in lipid metabolism regulation in NAFLD, prognosis of lung cancer, potential association with cardiovascular diseases, and osteoclast differentiation in Echinococcus granulosus-induced bone loss has been revealed through various research models. Understanding Camkk1 contributes to a better understanding of these biological processes and disease mechanisms, potentially providing new directions for treatment strategies.
References：
1. Yang, Fuji, Wu, Yanshuang, Chen, Yifei, Jin, Jianhua, Yan, Yongmin. 2023. Human umbilical cord mesenchymal stem cell-derived exosomes ameliorate liver steatosis by promoting fatty acid oxidation and reducing fatty acid synthesis. In JHEP reports : innovation in hepatology, 5, 100746. doi:10.1016/j.jhepr.2023.100746. https://pubmed.ncbi.nlm.nih.gov/37274776/
2. Zhang, Haorui, Chen, Bocen, Zou, Zixiu, Li, Feng, Wu, Junjie. 2021. Associations Between CAMKK1 Polymorphism rs7214723 and the Prognosis of Patients With Lung Cancer. In Frontiers in oncology, 11, 757484. doi:10.3389/fonc.2021.757484. https://pubmed.ncbi.nlm.nih.gov/34868969/
3. Beghi, Sofia, Cavaliere, Francesca, Manfredini, Matteo, Beghi, Cesare, Buschini, Annamaria. . Polymorphism rs7214723 in CAMKK1: a new genetic variant associated with cardiovascular diseases. In Bioscience reports, 41, . doi:10.1042/BSR20210326. https://pubmed.ncbi.nlm.nih.gov/34165505/
4. Petrvalska, Olivia, Honzejkova, Karolina, Koupilova, Nicola, Obsilova, Veronika, Obsil, Tomas. . 14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. In Protein science : a publication of the Protein Society, 32, e4805. doi:10.1002/pro.4805. https://pubmed.ncbi.nlm.nih.gov/37817008/
5. Huang, Yansheng, Huang, Yiping, Xiao, Jun, Ren, Qian, Wang, Sibo. 2024. Mechanisms of Nrf2 suppression and Camkk1 upregulation in Echinococcus granulosus-induced bone loss. In International journal of biological macromolecules, 288, 138521. doi:10.1016/j.ijbiomac.2024.138521. https://pubmed.ncbi.nlm.nih.gov/39674449/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen