C57BL/6JCya-Tle3em1/Cya
Common Name:
Tle3-KO
Product ID:
S-KO-17751
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tle3-KO
Strain ID
KOCMP-21887-Tle3-B6J-VA
Gene Name
Product ID
S-KO-17751
Gene Alias
2610103N05Rik; ESG; Grg3a; Grg3b; mKIAA1547
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tle3em1/Cya mice (Catalog S-KO-17751) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000160882
NCBI RefSeq
NM_001083927
Target Region
Exon 3~4
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Tle3, also known as Transducin-like enhancer of split 3, is a transcriptional cofactor or corepressor. It is involved in multiple signaling pathways and plays a crucial role in maintaining lineage fidelity in various cell types, and is associated with processes like cell differentiation, immune regulation, and cancer development. Genetic models, such as KO or CKO mouse models, have been essential for studying its functions.
In antigen-responding CD8+ T cells, genetic ablation of Tle3 promoted CD8+ TCM cell formation at the expense of CD8+ TEM cells, indicating its role in controlling effector and central memory CD8+ T cell fates [1]. In luminal breast cancer, TLE3 actively repressed the gene-expression signature associated with basal-like breast cancers, preventing a hybrid epithelial-mesenchymal state and reducing metastatic capacity [2]. Myeloid-specific knockout of Tle3 in mice led to increased numbers of regulatory T and TH17 cells in the colonic lamina propria, disrupting intestinal immune homeostasis [3]. Loss of TLE3 in LNCaP prostate cancer cells conferred resistance to AR antagonists [4]. In C2C12 myogenic cells, Tle3 depletion led to reduced expression of myogenic differentiation genes and impaired differentiation [5]. In colorectal cancer, TLE3 was down-regulated, and its overexpression repressed cancer cell proliferation by inhibiting MAPK and AKT signaling pathways [6]. Conditional deletion of TLE3 in adipocytes promoted mitochondrial oxidative metabolism and improved glucose control [7].
In conclusion, Tle3 is a key regulator in multiple biological processes. Model-based research, especially KO/CKO mouse models, has revealed its significance in diseases such as breast cancer, prostate cancer, colorectal cancer, and in immune-related disorders. These findings provide insights into potential therapeutic strategies targeting Tle3 for treating associated diseases.
References:
1. Zhao, Xin, Hu, Wei, Park, Sung Rye, Shan, Qiang, Xue, Hai-Hui. 2024. The transcriptional cofactor Tle3 reciprocally controls effector and central memory CD8+ T cell fates. In Nature immunology, 25, 294-306. doi:10.1038/s41590-023-01720-w. https://pubmed.ncbi.nlm.nih.gov/38238608/
2. Anstine, Lindsey J, Majmudar, Parth R, Aponte, Amy, Thompson, Cheryl L, Keri, Ruth A. . TLE3 Sustains Luminal Breast Cancer Lineage Fidelity to Suppress Metastasis. In Cancer research, 83, 997-1015. doi:10.1158/0008-5472.CAN-22-3133. https://pubmed.ncbi.nlm.nih.gov/36696357/
3. Li, Xiaoyu, Zhang, Bin, Zhang, Xiang, Xue, Hai-Hui, Hu, Xiaoyu. 2023. TLE3 and TLE4-coordinated colonic macrophage-CD4+ T cell crosstalk maintains intestinal immune homeostasis. In Mucosal immunology, 16, 50-60. doi:10.1016/j.mucimm.2022.12.005. https://pubmed.ncbi.nlm.nih.gov/36801171/
4. Palit, Sander Al, Vis, Daniel, Stelloo, Suzan, Zwart, Wilbert, van der Heijden, Michiel S. 2019. TLE3 loss confers AR inhibitor resistance by facilitating GR-mediated human prostate cancer cell growth. In eLife, 8, . doi:10.7554/eLife.47430. https://pubmed.ncbi.nlm.nih.gov/31855178/
5. Kumar, Pankaj, Zehra, Aatifa, Saini, Masum, Mathew, Sam J. . Zeb1 and Tle3 are trans-factors that differentially regulate the expression of myosin heavy chain-embryonic and skeletal muscle differentiation. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 37, e23074. doi:10.1096/fj.202201698RR. https://pubmed.ncbi.nlm.nih.gov/37392376/
6. Yang, Run-Wei, Zeng, Ying-Yue, Wei, Wen-Ting, Ding, Yan-Qing, Liao, Wen-Ting. 2016. TLE3 represses colorectal cancer proliferation by inhibiting MAPK and AKT signaling pathways. In Journal of experimental & clinical cancer research : CR, 35, 152. doi:. https://pubmed.ncbi.nlm.nih.gov/27669982/
7. Pearson, Stephanie, Loft, Anne, Rajbhandari, Prashant, Mandrup, Susanne, Villanueva, Claudio J. 2019. Loss of TLE3 promotes the mitochondrial program in beige adipocytes and improves glucose metabolism. In Genes & development, 33, 747-762. doi:10.1101/gad.321059.118. https://pubmed.ncbi.nlm.nih.gov/31123067/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen