C57BL/6JCya-Agpsem1/Cya
Common Name:
Agps-KO
Product ID:
S-KO-17954
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Agps-KO
Strain ID
KOCMP-228061-Agps-B6J-VA
Gene Name
Product ID
S-KO-17954
Gene Alias
5832437L22; 9930035G10Rik; Adaps; Adas; Adhaps; Adps; Aldhpsy; bs2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Agpsem1/Cya mice (Catalog S-KO-17954) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000047232
NCBI RefSeq
NM_172666
Target Region
Exon 2~3
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
Agps, or Arabinogalactan-proteins, are proteoglycans that are major components of the plant cell wall [2,3,4,5,6]. They play crucial roles in plant growth and development, including fruit ripening, reproductive development, and response to fungal infection [2,4,6]. In plants, they may be involved in processes such as cell division, root system development, and cell wall assembly through their unique structural features and interactions with other cell wall components [3].
In the context of prostate cancer, AGPS protein expression is downregulated in cancer tissues compared to normal tissues. Lower expression is associated with poorer overall survival of patients. AGPS can promote ferroptosis by modulating peroxisome function, leading to reduced survival of prostate cancer cells. Additionally, AGPS can be ubiquitinated and degraded by the E3 ligase-MDM2 through the proteasomal pathway, and kinase TrkA can promote this process by phosphorylating AGPS at the Y451 site. The TrkA inhibitor-Larotrectinib can increase the susceptibility of prostate cancer cells to ferroptosis, inhibiting cancer cell proliferation in vitro and in vivo [1].
In summary, Agps are important in plant growth and development. In prostate cancer, the down-regulation of AGPS and its regulation by TrkA and MDM2 play significant roles in cancer progression, suggesting that targeting this pathway could potentially be a new strategy for prostate cancer treatment.
References:
1. Zhang, Yu, Huang, Zhenlin, Li, Keqiang, Yang, Jinjian, Jia, Zhankui. 2024. TrkA promotes MDM2-mediated AGPS ubiquitination and degradation to trigger prostate cancer progression. In Journal of experimental & clinical cancer research : CR, 43, 16. doi:10.1186/s13046-023-02920-w. https://pubmed.ncbi.nlm.nih.gov/38200609/
2. Leszczuk, Agata, Kalaitzis, Panagiotis, Blazakis, Konstantinos N, Zdunek, Artur. 2020. The role of arabinogalactan proteins (AGPs) in fruit ripening-a review. In Horticulture research, 7, 176. doi:10.1038/s41438-020-00397-8. https://pubmed.ncbi.nlm.nih.gov/33328442/
3. Leszczuk, Agata, Kalaitzis, Panagiotis, Kulik, Joanna, Zdunek, Artur. 2023. Review: structure and modifications of arabinogalactan proteins (AGPs). In BMC plant biology, 23, 45. doi:10.1186/s12870-023-04066-5. https://pubmed.ncbi.nlm.nih.gov/36670377/
4. Lopes, Ana Lúcia, Moreira, Diana, Pereira, Ana Marta, Colombo, Lucia, Coimbra, Sílvia. . AGPs as molecular determinants of reproductive development. In Annals of botany, 131, 827-838. doi:10.1093/aob/mcad046. https://pubmed.ncbi.nlm.nih.gov/36945741/
5. Kutyrieva-Nowak, Nataliia, Leszczuk, Agata, Zdunek, Artur. 2023. A practical guide to in situ and ex situ characterisation of arabinogalactan proteins (AGPs) in fruits. In Plant methods, 19, 117. doi:10.1186/s13007-023-01100-3. https://pubmed.ncbi.nlm.nih.gov/37915041/
6. Leszczuk, Agata, Zając, Adrian, Cybulska, Justyna, Stefaniuk, Dawid, Zdunek, Artur. 2022. Working towards arabinogalactan proteins (AGPs) from fruit: carbohydrate composition and impact on fungal growth. In BMC plant biology, 22, 600. doi:10.1186/s12870-022-04009-6. https://pubmed.ncbi.nlm.nih.gov/36539686/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen