C57BL/6JCya-Osbp2em1/Cya
Common Name:
Osbp2-KO
Product ID:
S-KO-18105
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Osbp2-KO
Strain ID
KOCMP-74309-Osbp2-B6J-VB
Gene Name
Product ID
S-KO-18105
Gene Alias
1700095P05Rik; C630001G20Rik; Gm244; ORP-4; OSBPL1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Osbp2em1/Cya mice (Catalog S-KO-18105) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000070552
NCBI RefSeq
NM_152818
Target Region
Exon 6~8
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Osbp2, also known as HLM, is a member of the oxysterol-binding protein (OSBP) family. It binds specifically to oxysterols like 7-ketocholesterol and is involved in processes related to lipid metabolism, as oxysterols influence various cellular functions such as sterol metabolism, lipid trafficking, apoptosis, and cell differentiation [4]. It has been found to be associated with multiple biological pathways, potentially including WNT, MTOR, MAPK, and NOTCH signaling pathways [1].
Osbp2 has been implicated in several diseases. In hepatocellular carcinoma (HCC), its overexpression is related to poor prognosis, immunotherapy resistance, and chemotherapy resistance. It can inhibit NK cell and TIL cell infiltration, and impair Type-I and II IFN responses [1]. In pancreatic ductal adenocarcinoma (PDAC), overexpression of Osbp2 promotes cell migration, invasion, proliferation, and chemotherapy resistance, and decreases apoptosis, potentially through epithelial-mesenchymal transition [3]. In opisthorchiasis-associated cholangiocarcinoma, it may serve as a molecular marker for tumor metastasis [6]. In chronic myeloid leukemia, it is expressed in a significant proportion of patients, suggesting a role in disease monitoring [2]. In age-related macular degeneration, allelic variants of Osbp2 gene may be involved in the pathogenesis of oxidative damage to the macula [9]. In ITP, its expression is lower in patients compared to normal subjects, and it may be used as an effective diagnostic biomarker [8]. In Pekin ducks, differential expression of Osbp2 between susceptible and resistant lines to DHAV-3 infection is associated with altered lipid homeostasis [5]. In Kashmir cattle, it is related to reproduction [7]. Knockdown of Osbp2 increased the anti-PV activities of minor enviroxime-like compounds in cells [10].
In conclusion, Osbp2 plays crucial roles in lipid-related cellular processes and is associated with multiple disease conditions, including various cancers, leukemia, ITP, age-related macular degeneration, and duck hepatitis A virus-3 infection in ducks. Functional studies, especially those involving loss-of-function models like knockdown in cells, have revealed its importance in disease-related biological processes, providing potential targets for therapeutic intervention.
References:
1. Wu, Qinjuan, Li, Xin, Long, Menghong, Xie, Xianfeng, Liu, Qing. 2023. Integrated analysis of histone lysine lactylation (Kla)-specific genes suggests that NR6A1, OSBP2 and UNC119B are novel therapeutic targets for hepatocellular carcinoma. In Scientific reports, 13, 18642. doi:10.1038/s41598-023-46057-4. https://pubmed.ncbi.nlm.nih.gov/37903971/
2. Henriques Silva, Nathalie, Vasconcellos Fournier, Marcia, Pimenta, Glicinia, Spector, Nelson, da Costa Carvalho, Maria da Gloria. . HLM/OSBP2 is expressed in chronic myeloid leukemia. In International journal of molecular medicine, 12, 663-6. doi:. https://pubmed.ncbi.nlm.nih.gov/12964051/
3. Huang, Shuai, Zhang, Xudong, Luo, Kai, Jiang, Jianhua, Li, Renfeng. 2022. Oxysterol-Binding Protein 2 Promotes Pancreatic Ductal Adenocarcinoma Progression Through Epithelial-Mesenchymal Transition. In Frontiers in oncology, 11, 762233. doi:10.3389/fonc.2021.762233. https://pubmed.ncbi.nlm.nih.gov/35127474/
4. Moreira, E F, Jaworski, C, Li, A, Rodriguez, I R. 2001. Molecular and biochemical characterization of a novel oxysterol-binding protein (OSBP2) highly expressed in retina. In The Journal of biological chemistry, 276, 18570-8. doi:. https://pubmed.ncbi.nlm.nih.gov/11278871/
5. Wang, Xia, Ding, Dingbang, Liu, Ying, Hou, Shuisheng, Zhang, Yunsheng. 2023. Plasma lipidome reveals susceptibility and resistance of Pekin ducks to DHAV-3. In International journal of biological macromolecules, 253, 127095. doi:10.1016/j.ijbiomac.2023.127095. https://pubmed.ncbi.nlm.nih.gov/37758112/
6. Loilome, Watcharin, Wechagama, Pairoj, Namwat, Nisana, Kuver, Rahul, Yongvanit, Puangrat. 2011. Expression of oxysterol binding protein isoforms in opisthorchiasis-associated cholangiocarcinoma: a potential molecular marker for tumor metastasis. In Parasitology international, 61, 136-9. doi:10.1016/j.parint.2011.07.003. https://pubmed.ncbi.nlm.nih.gov/21763455/
7. Ahmed, Zulfiqar, Xiang, Weixuan, Wang, Fuwen, Lei, Chuzhao, Xu, Dequan. 2024. Whole-genome resequencing deciphers patterns of genetic diversity, phylogeny, and evolutionary dynamics in Kashmir cattle. In Animal genetics, 55, 511-526. doi:10.1111/age.13434. https://pubmed.ncbi.nlm.nih.gov/38726735/
8. Wang, Ming-Jing, Song, Ying, Guo, Xiao-Qing, Xu, Yong-Gang, Hu, Xiao-Mei. 2022. The Construction of ITP Diagnostic Modeling Based on the Expressions of Hub Genes Associated with M1 Polarization of Macrophages. In Journal of inflammation research, 15, 5905-5915. doi:10.2147/JIR.S364414. https://pubmed.ncbi.nlm.nih.gov/36274827/
9. Torrini, Margherita, Marchese, Cristiana, Vanzetti, Mario, Garré, Cecilia, Mareni, Cristina. . Mutation analysis of oxisterol-binding-protein gene in patients with age-related macular degeneration. In Genetic testing, 11, 421-6. doi:10.1089/gte.2007.0021. https://pubmed.ncbi.nlm.nih.gov/18294060/
10. Arita, Minetaro, Kojima, Hirotatsu, Nagano, Tetsuo, Wakita, Takaji, Shimizu, Hiroyuki. 2013. Oxysterol-binding protein family I is the target of minor enviroxime-like compounds. In Journal of virology, 87, 4252-60. doi:10.1128/JVI.03546-12. https://pubmed.ncbi.nlm.nih.gov/23365445/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen