C57BL/6JCya-Prkdcem1/Cya
Common Name
Prkdc-KO
Product ID
S-KO-18238
Backgroud
C57BL/6JCya
Strain ID
KOCMP-19090-Prkdc-B6J-VA
When using this mouse strain in a publication, please cite “Prkdc-KO Mouse (Catalog S-KO-18238) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Prkdc-KO
Strain ID
KOCMP-19090-Prkdc-B6J-VA
Gene Name
Product ID
S-KO-18238
Gene Alias
DNA-PKcs, DNAPDcs, DNAPK, DNPK1, DOXNPH, HYRC1, XRCC7, dxnph, p460, scid, slip
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 16
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000023352
NCBI RefSeq
NM_011159.2
Target Region
Exon 2~3
Size of Effective Region
~261 bp
Overview of Gene Research
Prkdc, encoding the DNA-dependent protein kinase catalytic subunit (DNA-PKcs), is crucial for nonhomologous end-joining DNA repair, a pathway vital for maintaining genomic stability [3]. It is involved in multiple biological processes and has significant implications for various diseases [3,5]. Genetic models, such as gene knockout (KO) and conditional knockout (CKO) mouse models, are valuable for studying Prkdc's functions.
In osteosarcoma, loss-of-function experiments showed that the loss of Prkdc significantly increased sensitivity to doxorubicin (DOX), as Prkdc recruited and bound GDE2 to enhance GNAS stability, which activated AKT phosphorylation and conferred DOX resistance [1].
In gastric cancer, hsa_circ_0136666 upregulated Prkdc expression by sponging miR-375-3p, regulating immune checkpoint proteins, and driving PD-L1 phosphorylation and immune escape [2].
In skin wound healing, knockdown of circ_PRKDC promoted keratinocyte migration during wound healing through the miR-31/FBN1 axis [4].
In conclusion, Prkdc plays essential roles in DNA repair, and its dysregulation is associated with various diseases. KO/CKO mouse models have revealed its role in cancer chemoresistance, tumor immune escape, and skin wound healing, providing potential therapeutic targets for these disease areas.
References：
1. Zhang, Wenchao, Li, Wei, Yin, Chi, Tu, Chao, Li, Zhihong. . PRKDC Induces Chemoresistance in Osteosarcoma by Recruiting GDE2 to Stabilize GNAS and Activate AKT. In Cancer research, 84, 2873-2887. doi:10.1158/0008-5472.CAN-24-0163. https://pubmed.ncbi.nlm.nih.gov/38900943/
2. Miao, Zhenyan, Li, Jifei, Wang, Yu, Zheng, Lufeng, Xing, Yingying. 2023. Hsa_circ_0136666 stimulates gastric cancer progression and tumor immune escape by regulating the miR-375/PRKDC Axis and PD-L1 phosphorylation. In Molecular cancer, 22, 205. doi:10.1186/s12943-023-01883-y. https://pubmed.ncbi.nlm.nih.gov/38093288/
3. Yin, Yuting, He, Qinglian, Li, Yuling, Li, Ziqi, Zhu, Wei. 2020. Emerging functions of PRKDC in the initiation and progression of cancer. In Tumori, 107, 483-488. doi:10.1177/0300891620950472. https://pubmed.ncbi.nlm.nih.gov/32867618/
4. Han, Dawei, Liu, Wenhui, Li, Guangshuai, Liu, Linbo. 2021. Circ_PRKDC knockdown promotes skin wound healing by enhancing keratinocyte migration via miR-31/FBN1 axis. In Journal of molecular histology, 52, 681-691. doi:10.1007/s10735-021-09996-8. https://pubmed.ncbi.nlm.nih.gov/34143322/
5. Yang, Xiawei, Yang, Feng, Lan, Liugen, Li, Haibin, Sun, Xuyong. 2022. Potential value of PRKDC as a therapeutic target and prognostic biomarker in pan-cancer. In Medicine, 101, e29628. doi:10.1097/MD.0000000000029628. https://pubmed.ncbi.nlm.nih.gov/35801800/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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