C57BL/6JCya-Cux2em1/Cya
Common Name:
Cux2-KO
Product ID:
S-KO-18243
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Cux2-KO
Strain ID
KOCMP-13048-Cux2-B6J-VB
Gene Name
Product ID
S-KO-18243
Gene Alias
1700051K22Rik; Cutl2; Cux-2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cux2em1/Cya mice (Catalog S-KO-18243) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000086317
NCBI RefSeq
NM_007804
Target Region
Exon 5
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
Cux2, or Cut-like homeobox 2 gene, encodes a transcription factor. It plays crucial roles in multiple biological processes such as neuronal development including proliferation, dendrite branching, and synapse formation [4,5,7]. It is also involved in limb development, regulating the positioning of forelimb buds [6]. Associated pathways include those related to histone modification, epithelial-mesenchymal transition, and various signaling cascades like PI3K-AKT-mTOR [3]. Genetic models, especially mouse models, have been valuable in studying Cux2's functions.
In cancer research, Cux2 has distinct roles. In breast cancer, its high expression promotes tumorigenesis and progression via the CUX2/KDM5B/SOX17 axis. Inhibition of Cux2 suppresses cell malignant phenotypes [1]. In gliomas, Cux2 has low expression, and its overexpression represses cell proliferation, migration, and invasion by enhancing ADCY1 transcription [2]. In papillary thyroid cancer, Cux2 functions as an oncogene, and its silencing inhibits cell proliferation, colony formation, migration, invasion, and induces apoptosis [3]. In epilepsy research, Cux2 deficiency in mouse models causes facilitation of excitatory synaptic transmission onto the hippocampus and increased seizure susceptibility to kainate [4].
In conclusion, Cux2 is a key regulator in neuronal and limb development. In disease areas, especially cancer and epilepsy, research using gene knockout (KO) or conditional knockout (CKO) mouse models has revealed its significant roles. Understanding Cux2's functions provides insights into the mechanisms of these biological processes and diseases, potentially offering new therapeutic targets.
References:
1. Li, Lili, Zhu, Genbao, Tan, Kemeng, Chen, Jie, Ma, Chengquan. . CUX2/KDM5B/SOX17 Axis Affects the Occurrence and Development of Breast Cancer. In Endocrinology, 163, . doi:10.1210/endocr/bqac110. https://pubmed.ncbi.nlm.nih.gov/35881915/
2. Yao, Guojun, Le, Shihai, Min, Sufang, Cai, Chuanxing, Deng, Ling. 2022. CUX2 prevents the malignant progression of gliomas by enhancing ADCY1 transcription. In Experimental brain research, 240, 3153-3165. doi:10.1007/s00221-022-06481-w. https://pubmed.ncbi.nlm.nih.gov/36242624/
3. Sun, Yihan, Ye, Danrong, Li, Yuefeng, Wang, Ouchen, Zhang, Xiaohua. 2018. CUX2 functions as an oncogene in papillary thyroid cancer. In OncoTargets and therapy, 12, 217-224. doi:10.2147/OTT.S185710. https://pubmed.ncbi.nlm.nih.gov/30636884/
4. Suzuki, Toshimitsu, Tatsukawa, Tetsuya, Sudo, Genki, Moore, Adrian Walton, Yamakawa, Kazuhiro. 2022. CUX2 deficiency causes facilitation of excitatory synaptic transmission onto hippocampus and increased seizure susceptibility to kainate. In Scientific reports, 12, 6505. doi:10.1038/s41598-022-10715-w. https://pubmed.ncbi.nlm.nih.gov/35581205/
5. Yang, Hayoung, Kim, Jiwoo, Kim, Yujin, Sestan, Nenad, Shim, Sungbo. 2019. Cux2 expression regulated by Lhx2 in the upper layer neurons of the developing cortex. In Biochemical and biophysical research communications, 521, 874-879. doi:10.1016/j.bbrc.2019.11.004. https://pubmed.ncbi.nlm.nih.gov/31708105/
6. Ueda, Shogo, Cordeiro, Ingrid Rosenburg, Moriyama, Yuuta, Shirahige, Katsuhiko, Tanaka, Mikiko. 2019. Cux2 refines the forelimb field by controlling expression of Raldh2 and Hox genes. In Biology open, 8, . doi:10.1242/bio.040584. https://pubmed.ncbi.nlm.nih.gov/30651234/
7. Yamada, Makiko, Clark, Jessica, McClelland, Christine, Ray, Ayush, Iulianella, Angelo. 2014. Cux2 activity defines a subpopulation of perinatal neurogenic progenitors in the hippocampus. In Hippocampus, 25, 253-67. doi:10.1002/hipo.22370. https://pubmed.ncbi.nlm.nih.gov/25252086/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen