C57BL/6JCya-Cenpeem1/Cya
Common Name:
Cenpe-KO
Product ID:
S-KO-18246
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cenpe-KO
Strain ID
KOCMP-229841-Cenpe-B6J-VA
Gene Name
Product ID
S-KO-18246
Gene Alias
312kDa; C530022J18; CENP-E; Kif10
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cenpeem1/Cya mice (Catalog S-KO-18246) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000062893
NCBI RefSeq
NM_173762
Target Region
Exon 2~5
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Cenpe, also known as centromere protein E, is a microtubule plus-end-directed kinetochore protein belonging to the centromere protein (CENP) family. It plays a crucial role in mitosis, participating in processes related to the cell cycle. Mutations in Cenpe can lead to primary microcephaly syndrome [1,4].
In various diseases, Cenpe has been found to be involved. In non-WNT/non-SHH medulloblastoma, it is highly expressed and serves as an independent prognostic factor, and its knockdown inhibits cell proliferation by activating the p53 signaling pathway and blocking the cell cycle [1]. In pulmonary hypertension, Cenpe deficiency significantly inhibits the development of pulmonary vascular remodeling and right ventricular hypertrophy, and knocking out Cenpe inhibits the proliferation and induces apoptosis of primary pulmonary artery smooth muscle cells [2]. In cervical cancer, its up-regulation promotes cancer progression through IL-6-mediated PI3K-Akt and MAPK signaling pathways, and it shows high diagnostic accuracy [3]. In medulloblastoma cells, CENPE knockdown induces mitotic defects, apoptosis, and endogenous DNA damage accumulation [4]. In glioblastoma, CENPE promotes proliferation by directly binding to WEE1 and regulating cell cycle transitions [5]. In chemoresistant acute myeloid leukemia, knockdown of CENPE suppresses cell proliferation and reverses chemoresistance, and Lin28A is found to be correlated with CENPE expression [6]. In esophageal adenocarcinoma, high CENPE expression is associated with unfavorable overall survival, and its expression is related to DNA methylation status [7]. In chromophobe renal cell carcinoma, CENPE is a potential prognostic biomarker and is enriched in proliferation-related pathways [8]. In non-small cell lung cancer, high CENPE expression is related to poor prognosis [9]. In lung adenocarcinoma, CENPE promotes cell proliferation and is directly regulated by FOXM1 [10].
In conclusion, Cenpe is essential for normal cellular processes, especially those related to the cell cycle. Its dysregulation is associated with multiple diseases, and studies using loss-of-function models, such as knockdown experiments, have revealed its role in promoting tumor cell proliferation, affecting cell cycle regulation, and being involved in various signaling pathways in different cancers and other disease conditions.
References:
1. Fang, Huangyi, Zhang, Yusong, Lin, Chengyin, Sheng, Hansong, Lin, Jian. 2023. Primary microcephaly gene CENPE is a novel biomarker and potential therapeutic target for non-WNT/non-SHH medulloblastoma. In Frontiers in immunology, 14, 1227143. doi:10.3389/fimmu.2023.1227143. https://pubmed.ncbi.nlm.nih.gov/37593739/
2. Fang, Xiaoyu, Xie, Min, Liu, Xiansheng, He, Yuanzhou. 2021. CENPE contributes to pulmonary vascular remodeling in pulmonary hypertension. In Biochemical and biophysical research communications, 557, 40-47. doi:10.1016/j.bbrc.2021.04.010. https://pubmed.ncbi.nlm.nih.gov/33862458/
3. Peng, Peiqiang, Zheng, Jingying, He, Kang, Zhang, Shuang, Zhao, Lijing. 2024. CENPE is a diagnostic and prognostic biomarker for cervical cancer. In Heliyon, 10, e40860. doi:10.1016/j.heliyon.2024.e40860. https://pubmed.ncbi.nlm.nih.gov/39759304/
4. Iegiani, Giorgia, Gai, Marta, Di Cunto, Ferdinando, Pallavicini, Gianmarco. 2021. CENPE Inhibition Leads to Mitotic Catastrophe and DNA Damage in Medulloblastoma Cells. In Cancers, 13, . doi:10.3390/cancers13051028. https://pubmed.ncbi.nlm.nih.gov/33804489/
5. Ma, Chuanling, Wang, Jianchao, Zhou, Jie, Li, Fangqiong, Zhang, Meng. . CENPE promotes glioblastomas proliferation by directly binding to WEE1. In Translational cancer research, 9, 717-725. doi:10.21037/tcr.2019.11.40. https://pubmed.ncbi.nlm.nih.gov/35117417/
6. Shi, Mingyue, Niu, Junwei, Niu, Xiaona, Chen, Yuqing, Shao, Fengmin. 2021. Lin28A/CENPE Promoting the Proliferation and Chemoresistance of Acute Myeloid Leukemia. In Frontiers in oncology, 11, 763232. doi:10.3389/fonc.2021.763232. https://pubmed.ncbi.nlm.nih.gov/34868981/
7. Zhu, Xueqiang, Luo, Xing, Feng, Gang, Zeng, Ming, Liu, Hao. 2019. CENPE expression is associated with its DNA methylation status in esophageal adenocarcinoma and independently predicts unfavorable overall survival. In PloS one, 14, e0207341. doi:10.1371/journal.pone.0207341. https://pubmed.ncbi.nlm.nih.gov/30716092/
8. Wu, Hui-Feng, Liu, Hao, Zhang, Zhe-Wei, Chen, Ji-Min. 2023. CENPE and LDHA were potential prognostic biomarkers of chromophobe renal cell carcinoma. In European journal of medical research, 28, 481. doi:10.1186/s40001-023-01449-0. https://pubmed.ncbi.nlm.nih.gov/37925501/
9. Hao, Xuezhi, Qu, Tao. 2019. Expression of CENPE and its Prognostic Role in Non-small Cell Lung Cancer. In Open medicine (Warsaw, Poland), 14, 497-502. doi:10.1515/med-2019-0053. https://pubmed.ncbi.nlm.nih.gov/31259255/
10. Shan, Lina, Zhao, Minjie, Lu, Ya, Chai, Wenshu, Shi, Xianbao. 2019. CENPE promotes lung adenocarcinoma proliferation and is directly regulated by FOXM1. In International journal of oncology, 55, 257-266. doi:10.3892/ijo.2019.4805. https://pubmed.ncbi.nlm.nih.gov/31115500/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen