C57BL/6JCya-Cdc27em1/Cya
Common Name:
Cdc27-KO
Product ID:
S-KO-18276
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cdc27-KO
Strain ID
KOCMP-217232-Cdc27-B6J-VB
Gene Name
Product ID
S-KO-18276
Gene Alias
APC3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cdc27em1/Cya mice (Catalog S-KO-18276) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000093923
NCBI RefSeq
NM_145436
Target Region
Exon 6~9
Size of Effective Region
~3.8 kb
Detailed Document
Overview of Gene Research
Cdc27, a core component of the Anaphase Promoting complex/cyclosome, plays a crucial role in controlling cell cycle transitions during cellular division. It is involved in the regulation of key processes related to the cell cycle, which are essential for normal cell growth and development [1].
CDC27 has been implicated in various diseases. In cancer, its role seems dual-acting as either a tumor suppressor gene or an oncogene in different neoplasms. In neuroblastoma, it promotes cell growth, metastasis, and sphere-formation ability in an ODC1-dependent manner and also affects ferroptosis [2]. In T-cell lymphoblastic lymphoma, it promotes proliferation, facilitates G1/S transition, inhibits apoptosis, and has a positive correlation with PD-L1 expression [3]. In acute leukemia, patients have significantly higher CDC27 expression levels, suggesting its potential as an oncogene and a biomarker [4]. In gastric and colorectal cancer, it promotes cell proliferation, invasion, and metastasis via epithelial-mesenchymal transition [7,10]. In breast cancer, low cdc27 expression is associated with a poor prognosis [8]. In contrast, in systemic lupus erythematosus (SLE), CDC27 expression is low in lupus patients and is related to disease activity [5]. Also, CDC27 has been identified as a candidate gene for hemifacial microsomia, with its knockout in zebrafish causing craniofacial malformation and other HFM-like phenotypes [6]. Additionally, in idiopathic pulmonary fibrosis, lncRNA TUG1 promotes fibrosis by up-regulating CDC27 and activating the PI3K/Akt/mTOR pathway [9].
In conclusion, Cdc27 is essential for cell cycle regulation. Its dysregulation is associated with multiple diseases, especially various cancers, SLE, and hemifacial microsomia. Studies using different models, such as gene knockout in zebrafish, have provided insights into its role in these disease conditions, highlighting its potential as a biomarker and therapeutic target.
References:
1. Kazemi-Sefat, Golnaz Ensieh, Keramatipour, Mohammad, Talebi, Saeed, Kazemi-Sefat, Nazanin Atieh, Mousavizadeh, Kazem. 2021. The importance of CDC27 in cancer: molecular pathology and clinical aspects. In Cancer cell international, 21, 160. doi:10.1186/s12935-021-01860-9. https://pubmed.ncbi.nlm.nih.gov/33750395/
2. Qiu, Lin, Zhou, Rui, Luo, Ziyan, Wu, Jiangxue, Jiang, Hua. 2022. CDC27-ODC1 Axis Promotes Metastasis, Accelerates Ferroptosis and Predicts Poor Prognosis in Neuroblastoma. In Frontiers in oncology, 12, 774458. doi:10.3389/fonc.2022.774458. https://pubmed.ncbi.nlm.nih.gov/35242701/
3. Song, Yue, Song, Wei, Li, Zhaoming, Xia, Qingxin, Zhang, Mingzhi. 2020. CDC27 Promotes Tumor Progression and Affects PD-L1 Expression in T-Cell Lymphoblastic Lymphoma. In Frontiers in oncology, 10, 488. doi:10.3389/fonc.2020.00488. https://pubmed.ncbi.nlm.nih.gov/32391258/
4. Pouriafar, Yasaman, Rostami, Shahrbano, Alizadghandforoush, Nasrin, Amini, Ali, Safa, Majid. 2024. CDC27 gene expression patterns as a potential biomarker in Acute Leukemia. In Molecular biology reports, 51, 865. doi:10.1007/s11033-024-09744-7. https://pubmed.ncbi.nlm.nih.gov/39073611/
5. Shang, Shunlai, Zhou, Yena, Chen, Keng, Chen, Xiangmei, Li, Qinggang. 2022. A Novel Gene CDC27 Causes SLE and Is Associated With the Disease Activity. In Frontiers in immunology, 13, 876963. doi:10.3389/fimmu.2022.876963. https://pubmed.ncbi.nlm.nih.gov/35418986/
6. Song, Wenjie, Xia, Xin, Fan, Yue, Zhang, Bo, Chen, Xiaowei. 2024. Functional and Genetic Analyses Unveil the Implication of CDC27 in Hemifacial Microsomia. In International journal of molecular sciences, 25, . doi:10.3390/ijms25094707. https://pubmed.ncbi.nlm.nih.gov/38731925/
7. Xin, Yongfan, Ning, Shili, Zhang, Liang, Cui, Ming. 2018. CDC27 Facilitates Gastric Cancer Cell Proliferation, Invasion and Metastasis via Twist-Induced Epithelial-Mesenchymal Transition. In Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 50, 501-511. doi:10.1159/000494164. https://pubmed.ncbi.nlm.nih.gov/30308498/
8. Talvinen, Kati, Karra, Henna, Pitkänen, Reino, Kuopio, Teijo, Kronqvist, Pauliina. 2013. Low cdc27 and high securin expression predict short survival for breast cancer patients. In APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 121, 945-53. doi:10.1111/apm.12110. https://pubmed.ncbi.nlm.nih.gov/23755904/
9. Qi, Fei, Lv, Zhong-Dong, Huang, Wen-Di, Liu, Xin-Min, Song, Wei-Dong. . LncRNA TUG1 promotes pulmonary fibrosis progression via up-regulating CDC27 and activating PI3K/Akt/mTOR pathway. In Epigenetics, 18, 2195305. doi:10.1080/15592294.2023.2195305. https://pubmed.ncbi.nlm.nih.gov/36994860/
10. Qiu, Lin, Tan, Xin, Lin, Jiaxin, Wu, Jiangxue, Huang, Wenlin. 2017. CDC27 Induces Metastasis and Invasion in Colorectal Cancer via the Promotion of Epithelial-To-Mesenchymal Transition. In Journal of Cancer, 8, 2626-2635. doi:10.7150/jca.19381. https://pubmed.ncbi.nlm.nih.gov/28900500/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen