C57BL/6JCya-Mgst2em1/Cya
Common Name:
Mgst2-KO
Product ID:
S-KO-18353
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Mgst2-KO
Strain ID
KOCMP-211666-Mgst2-B6J-VA
Gene Name
Product ID
S-KO-18353
Gene Alias
GST2; MGST-II
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mgst2em1/Cya mice (Catalog S-KO-18353) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000099106
NCBI RefSeq
NM_174995
Target Region
Exon 3
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
Mgst2, microsomal glutathione S-transferase 2, is a member of the MAPEG superfamily. It is involved in leukotriene C4 production, which is crucial for intracrine signaling of endoplasmic reticulum stress, oxidative DNA damage, and cell death. It also participates in the regulation of the oxidative stress pathway [1,2].
In a study, Mgst2-gene-deficient mice were generated by Nuclease technology-mediated gene editing. Morphometric analysis showed that the height, width, and volume of the eyeballs in Mgst2 KO homozygous mice were significantly greater than those of wild-type mice, indicating a potential contribution to the development of a subgroup of strabismus. Additionally, Mgst2 was identified as a candidate gene for comitant strabismus susceptibility in Japanese patients [3,4]. In another study, coniferyl ferulate was found to alleviate xylene-caused hematopoietic stem and progenitor cell toxicity by targeting Mgst2, especially in neutrophil progenitor subpopulations [5].
In conclusion, Mgst2 plays essential roles in leukotriene C4 synthesis and oxidative stress regulation. The Mgst2-KO mouse model has provided insights into its role in eye development and strabismus. Research on Mgst2 also reveals its potential as a therapeutic target for xylene-induced hematotoxicity.
References:
1. Thulasingam, Madhuranayaki, Orellana, Laura, Nji, Emmanuel, Rinaldo-Matthis, Agnes, Haeggström, Jesper Z. 2021. Crystal structures of human MGST2 reveal synchronized conformational changes regulating catalysis. In Nature communications, 12, 1728. doi:10.1038/s41467-021-21924-8. https://pubmed.ncbi.nlm.nih.gov/33741927/
2. Zhao, Guangdong, Zhao, Wenchun, Cui, Xuepei, Li, Han, Guo, Xingqi. 2021. Identification of an MGST2 gene and analysis of its function in antioxidant processes in Apis cerana cerana. In Archives of insect biochemistry and physiology, 106, e21770. doi:10.1002/arch.21770. https://pubmed.ncbi.nlm.nih.gov/33660279/
3. Zhang, Jingjing, Matsuo, Toshihiko. 2017. MGST2 and WNT2 are candidate genes for comitant strabismus susceptibility in Japanese patients. In PeerJ, 5, e3935. doi:10.7717/peerj.3935. https://pubmed.ncbi.nlm.nih.gov/29062608/
4. Matsuo, Toshihiko, Sugimoto, Kohei, Miyaji, Mary, Horii, Takuro, Hatada, Izuho. 2024. Morphometric Analysis of the Eye by Magnetic Resonance Imaging in MGST2-Gene-Deficient Mice. In Biomedicines, 12, . doi:10.3390/biomedicines12020370. https://pubmed.ncbi.nlm.nih.gov/38397974/
5. Yin, Zhao, Ou, Ruiming, Zhu, Yangmin, Zhang, Qing, Liu, Shuang. 2024. Coniferyl ferulate alleviate xylene-caused hematopoietic stem and progenitor cell toxicity by Mgst2. In Frontiers in pharmacology, 15, 1334445. doi:10.3389/fphar.2024.1334445. https://pubmed.ncbi.nlm.nih.gov/38523643/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen