C57BL/6JCya-Foxc2em1/Cya
Common Name:
Foxc2-KO
Product ID:
S-KO-18397
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Foxc2-KO
Strain ID
KOCMP-14234-Foxc2-B6J-VA
Gene Name
Product ID
S-KO-18397
Gene Alias
Fkh14; Hfhbf3; MFH-1; Mfh1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Foxc2em1/Cya mice (Catalog S-KO-18397) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000054691
NCBI RefSeq
NM_013519
Target Region
Exon 1
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Foxc2, a member of the forkhead box family of transcription factors, is crucial for embryonic and prenatal development. It acts as a key modulator in angiogenesis and lymphangiogenesis via multiple related pathways [7].
In cancer research, Foxc2 has emerged as an oncogene. High Foxc2 expression in human solid tumors is associated with shorter overall survival, lymph node metastases, TNM stage, and age, making it a promising prognostic and target marker [3]. It promotes vasculogenic mimicry (VM) in diverse solid tumors, driving ectopic expression of endothelial genes in tumor cells under hypoxia, and VM-proficient tumors are resistant to anti-angiogenic therapy [1,6]. Additionally, Foxc2 contributes to chemoresistance in cancer by promoting epithelial-mesenchymal transition, inducing multidrug transporters, activating the oxidative stress response, and deregulating cell survival signaling pathways [2]. In ovarian cancer, it promotes metastasis, lipid metabolism, and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis [4]. In hepatic stellate cells, the FOXC2-AS1/FOXC2 axis mediates matrix stiffness-induced trans-differentiation into fibrosis-promoting myofibroblasts [5].
In conclusion, Foxc2 plays essential roles in embryonic development, angiogenesis, and lymphangiogenesis. Its dysregulation is closely associated with various cancer-related processes, such as metastasis, chemoresistance, and vasculogenic mimicry. Research on Foxc2 using in vivo models like KO/CKO mouse models has provided valuable insights into its function in these disease-related biological processes, guiding potential prognostic and therapeutic strategies in cancer treatment.
References:
1. Cannell, Ian G, Sawicka, Kirsty, Pearsall, Isabella, Caldas, Carlos, Hannon, Gregory J. 2023. FOXC2 promotes vasculogenic mimicry and resistance to anti-angiogenic therapy. In Cell reports, 42, 112791. doi:10.1016/j.celrep.2023.112791. https://pubmed.ncbi.nlm.nih.gov/37499655/
2. Hargadon, Kristian M, Strong, Elijah W. . The FOXC2 Transcription Factor: A Master Regulator of Chemoresistance in Cancer. In Technology in cancer research & treatment, 22, 15330338231155284. doi:10.1177/15330338231155284. https://pubmed.ncbi.nlm.nih.gov/36740986/
3. Zhang, Long, He, Yong, Tu, Xiaohong, Jiang, Yufen, Deng, Xiaohong. 2022. FOXC2 as a prognostic marker and a potential molecular target in patients with human solid tumors. In Frontiers in surgery, 9, 960698. doi:10.3389/fsurg.2022.960698. https://pubmed.ncbi.nlm.nih.gov/36425886/
4. Lin, Feikai, Li, Xiaoduan, Wang, Xinjing, Wang, Ziliang, Wang, Xipeng. 2022. Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer. In Journal of experimental & clinical cancer research : CR, 41, 129. doi:10.1186/s13046-022-02315-3. https://pubmed.ncbi.nlm.nih.gov/35392966/
5. Sun, Liankang, Li, Yue, Wang, Hao, Tu, Kangsheng, Shi, Yu. 2023. FOXC2-AS1/FOXC2 axis mediates matrix stiffness-induced trans-differentiation of hepatic stellate cells into fibrosis-promoting myofibroblasts. In International journal of biological sciences, 19, 4206-4222. doi:10.7150/ijbs.81581. https://pubmed.ncbi.nlm.nih.gov/37705741/
6. Recouvreux, Maria Sol, Miao, Jiangyong, Gozo, Maricel C, Karlan, Beth Y, Orsulic, Sandra. 2022. FOXC2 Promotes Vasculogenic Mimicry in Ovarian Cancer. In Cancers, 14, . doi:10.3390/cancers14194851. https://pubmed.ncbi.nlm.nih.gov/36230774/
7. Wang, Teng, Zheng, Lei, Wang, Qian, Hu, Yan-Wei. 2018. Emerging roles and mechanisms of FOXC2 in cancer. In Clinica chimica acta; international journal of clinical chemistry, 479, 84-93. doi:10.1016/j.cca.2018.01.019. https://pubmed.ncbi.nlm.nih.gov/29341903/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen