C57BL/6JCya-Tmem43em1/Cya
Common Name:
Tmem43-KO
Product ID:
S-KO-18407
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Tmem43-KO
Strain ID
KOCMP-74122-Tmem43-B6J-VB
Gene Name
Product ID
S-KO-18407
Gene Alias
1200015A22Rik; LUMA
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmem43em1/Cya mice (Catalog S-KO-18407) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032183
NCBI RefSeq
NM_028766
Target Region
Exon 5~7
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
TMEM43, also known as LUMA, is a transmembrane protein encoded by a highly conserved gene and widely expressed across species [2]. It has been implicated in various biological processes and diseases. TMEM43 is associated with pathways related to cardiac function, metabolism, and cancer development, and its study via genetic models can provide insights into its functions.
In mouse models, TMEM43 knockdown in hearts deteriorated LPS-induced cardiac injury and dysfunction, while overexpression alleviated these effects, suggesting it protects against sepsis-induced cardiac injury by inhibiting ferroptosis [1]. The Ser358Leu mutation of TMEM43 in mice led to hyper-activated nuclear factor κB (NF-κB) activation, driving the expression of pro-fibrotic gene TGFβ1, and enhancing downstream signals in arrhythmogenic right ventricular dysplasia/cardiomyopathy [3]. Additionally, Tmem43 S358L mutant mice displayed systolic dysfunction, arrhythmias, fibro-fatty infiltration in the heart, and abnormal intestinal phenotypes, with disrupted WNT-β-catenin and PPARG signaling [4].
In conclusion, TMEM43 plays a crucial role in maintaining cardiac and metabolic homeostasis. Mouse models, especially those with TMEM43 knockout or specific mutations, have revealed its significance in sepsis-induced cardiac injury and arrhythmogenic cardiomyopathy. These findings enhance our understanding of related disease mechanisms and may provide potential therapeutic targets.
References:
1. Chen, Zhen, Cao, Zhe, Gui, Feng, Ai, Fen, Zhang, Jun. 2022. TMEM43 Protects against Sepsis-Induced Cardiac Injury via Inhibiting Ferroptosis in Mice. In Cells, 11, . doi:10.3390/cells11192992. https://pubmed.ncbi.nlm.nih.gov/36230956/
2. Zhang, Nannan, Wang, Feiran, Yang, Xiaobing, Feitelson, Mark A, Chen, Zhong. 2024. TMEM43 promotes the development of hepatocellular carcinoma by activating VDAC1 through USP7 deubiquitination. In Translational gastroenterology and hepatology, 9, 9. doi:10.21037/tgh-23-108. https://pubmed.ncbi.nlm.nih.gov/38317750/
3. Zheng, Guoxing, Jiang, Changying, Li, Yulin, Du, Jie, Lin, Xin. 2018. TMEM43-S358L mutation enhances NF-κB-TGFβ signal cascade in arrhythmogenic right ventricular dysplasia/cardiomyopathy. In Protein & cell, 10, 104-119. doi:10.1007/s13238-018-0563-2. https://pubmed.ncbi.nlm.nih.gov/29980933/
4. Orgil, Buyan-Ochir, Munkhsaikhan, Undral, Pierre, Joseph F, Towbin, Jeffrey A, Purevjav, Enkhsaikhan. 2023. The TMEM43 S358L mutation affects cardiac, small intestine, and metabolic homeostasis in a knock-in mouse model. In American journal of physiology. Heart and circulatory physiology, 324, H866-H880. doi:10.1152/ajpheart.00712.2022. https://pubmed.ncbi.nlm.nih.gov/37083466/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen