C57BL/6JCya-Cers1em1/Cya
Common Name:
Cers1-KO
Product ID:
S-KO-18446
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cers1-KO
Strain ID
KOCMP-93898-Cers1-B6J-VB
Gene Name
Product ID
S-KO-18446
Gene Alias
Lass1; Uog-1; to
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cers1em1/Cya mice (Catalog S-KO-18446) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000140239
NCBI RefSeq
NM_138647
Target Region
Exon 2
Size of Effective Region
~0.2 kb
Detailed Document
Overview of Gene Research
Cers1, short for ceramide synthase 1, is a key enzyme in the de novo sphingolipid synthesis pathway. It is responsible for generating C18:0 ceramide, a central molecule in sphingolipid metabolism that has important functions in membrane structure and cellular signaling [1,2,3,4]. The sphingolipid biosynthesis pathway, in which Cers1 is involved, is crucial for various biological processes such as cell differentiation, apoptosis, and mitochondrial function [1,6]. Genetic models, like gene knockout (KO) or conditional knockout (CKO) mouse models, have been valuable in studying Cers1's functions.
In skeletal muscle, Cers1 seems to play a significant role in age-related muscle function and insulin resistance. Inhibition of Cers1 in aged mice, either pharmacologically or genetically, exacerbates age-related muscle dysfunction, including blunted myogenesis, deteriorated muscle mass and function, along with features of inflammation and fibrosis [2]. Additionally, mice lacking Cers1, either globally or specifically in skeletal muscle (CerS1ΔSkM), show reduced muscle C18:0 ceramide content and improved systemic glucose homeostasis, protecting against obesity-induced insulin resistance [3]. In hypophysoma, over-expression of Cers1 by lentivirus can inhibit the development of hypophysoma in vivo and in vitro, possibly by enhancing autophagy through the PI3K/AKT signaling pathway [5].
In conclusion, Cers1 is essential in regulating sphingolipid metabolism with far-reaching impacts on multiple biological processes. Model-based research, especially using KO/CKO mouse models, has revealed its crucial roles in age-related muscle homeostasis and insulin resistance-associated diseases. Understanding Cers1 provides insights into the mechanisms underlying these conditions and may offer potential therapeutic targets.
References:
1. Boyd, Rowan A, Majumder, Saurav, Stiban, Johnny, Obeid, Lina M, Senkal, Can E. 2023. The heat shock protein Hsp27 controls mitochondrial function by modulating ceramide generation. In Cell reports, 42, 113081. doi:10.1016/j.celrep.2023.113081. https://pubmed.ncbi.nlm.nih.gov/37689067/
2. Wohlwend, Martin, Laurila, Pirkka-Pekka, Goeminne, Ludger J E, Ivanisevic, Julijana, Auwerx, Johan. 2024. Inhibition of CERS1 in skeletal muscle exacerbates age-related muscle dysfunction. In eLife, 12, . doi:10.7554/eLife.90522. https://pubmed.ncbi.nlm.nih.gov/38506902/
3. Turpin-Nolan, Sarah M, Hammerschmidt, Philipp, Chen, Weiyi, Brodesser, Susanne, Brüning, Jens C. . CerS1-Derived C18:0 Ceramide in Skeletal Muscle Promotes Obesity-Induced Insulin Resistance. In Cell reports, 26, 1-10.e7. doi:10.1016/j.celrep.2018.12.031. https://pubmed.ncbi.nlm.nih.gov/30605666/
4. Błachnio-Zabielska, Agnieszka U, Roszczyc-Owsiejczuk, Kamila, Imierska, Monika, Daniluk, Jarosław, Zabielski, Piotr. 2022. CerS1 but Not CerS5 Gene Silencing, Improves Insulin Sensitivity and Glucose Uptake in Skeletal Muscle. In Cells, 11, . doi:10.3390/cells11020206. https://pubmed.ncbi.nlm.nih.gov/35053322/
5. Wang, Jingtao, Zhang, Jimin, Ma, Dongzhou, Li, Xiushan. . The Potential Role of CERS1 in Autophagy Through PI3K/AKT Signaling Pathway in Hypophysoma. In Technology in cancer research & treatment, 19, 1533033820977536. doi:10.1177/1533033820977536. https://pubmed.ncbi.nlm.nih.gov/33267708/
6. Oleinik, Natalia, Albayram, Onder, Kassir, Mohamed Faisal, Szulc, Zdzislaw M, Ogretmen, Besim. 2023. Alterations of lipid-mediated mitophagy result in aging-dependent sensorimotor defects. In Aging cell, 22, e13954. doi:10.1111/acel.13954. https://pubmed.ncbi.nlm.nih.gov/37614052/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen