C57BL/6JCya-Atg7em1/Cya
Common Name:
Atg7-KO
Product ID:
S-KO-18459
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Atg7-KO
Strain ID
KOCMP-74244-Atg7-B6J-VA
Gene Name
Product ID
S-KO-18459
Gene Alias
1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Atg7em1/Cya mice (Catalog S-KO-18459) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000169310
NCBI RefSeq
NM_001253717
Target Region
Exon 5~6
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Atg7, an essential autophagy-related gene, acts as an E1-like activating enzyme. It is crucial for classical degradative macroautophagy/autophagy, facilitating both microtubule-associated protein light chain 3 (LC3)-phosphatidylethanolamine and ATG12 conjugation. Thus, it stands at the hub of two ubiquitin-like systems in autophagic vesicle expansion. It is also involved in regulating cell cycle, apoptosis, immunity, and protein secretion, and plays a significant role in human health and disease [6,7].
In adipose-selective fto knockout mice, FTO deficiency decreased white fat mass and impaired ATG7-dependent autophagy in vivo, indicating that m6A mRNA methylation controls autophagy and adipogenesis by targeting Atg7 [1]. In Chk2-/-mice, an aggravated infarction phenotype was observed along with reduced phosphorylation of TRIM32 and ubiquitination of ATG7 in a stroke model, suggesting that the ATM-CHK2-TRIM32 axis regulates ATG7 ubiquitination to initiate autophagy under oxidative stress [2]. Endothelial cell-specific atg7 knockout (atg7 KO) mice showed impaired angiogenesis, delayed blood flow reperfusion, and reduced HIF1A expression, revealing that lack of ATG7 inhibits angiogenesis by suppressing HIF1A expression through upregulating STAT1 independently of autophagy under ischemic conditions [3]. TRIM7-deficient mice had elevated innate immune responses and increased L. monocytogenes infection, as TRIM7 promotes autophagy via regulation of ATG7 ubiquitination during infection [4]. Conditional knockout of Atg7 in endothelial cells (Atg7-ECKO) led to astrocyte-microvascular disassociation and BBB leakage in mice, suggesting that endothelial Atg7 is essential for maintaining BBB integrity [5].
In conclusion, Atg7 is a key regulator in multiple biological processes. Gene knockout and conditional knockout mouse models have been instrumental in uncovering its role in autophagy, adipogenesis, angiogenesis, oxidative stress response, anti-bacterial defense, and blood-brain barrier maintenance. These findings contribute to understanding the mechanisms underlying various diseases such as obesity, stroke, ischemic diseases, intracellular bacterial infections, and potentially neurological disorders related to BBB dysfunction.
References:
1. Wang, Xinxia, Wu, Ruifan, Liu, Youhua, Wang, Fengqin, Wang, Yizhen. 2019. m6A mRNA methylation controls autophagy and adipogenesis by targeting Atg5 and Atg7. In Autophagy, 16, 1221-1235. doi:10.1080/15548627.2019.1659617. https://pubmed.ncbi.nlm.nih.gov/31451060/
2. Liu, Jingwei, Lu, Songming, Zheng, Lixia, Wang, Zhenning, Cao, Liu. 2023. ATM-CHK2-TRIM32 axis regulates ATG7 ubiquitination to initiate autophagy under oxidative stress. In Cell reports, 42, 113402. doi:10.1016/j.celrep.2023.113402. https://pubmed.ncbi.nlm.nih.gov/37943659/
3. Yao, Hongmin, Li, Jian, Liu, Zhixue, Mu, Jing, Xie, Zhonglin. 2022. Ablation of endothelial Atg7 inhibits ischemia-induced angiogenesis by upregulating Stat1 that suppresses Hif1a expression. In Autophagy, 19, 1491-1511. doi:10.1080/15548627.2022.2139920. https://pubmed.ncbi.nlm.nih.gov/36300763/
4. Wang, Jie, Qin, Xiao, Huang, Yulu, Wang, Hui, Yang, Bo. 2023. TRIM7/RNF90 promotes autophagy via regulation of ATG7 ubiquitination during L. monocytogenes infection. In Autophagy, 19, 1844-1862. doi:10.1080/15548627.2022.2162706. https://pubmed.ncbi.nlm.nih.gov/36576150/
5. Liu, Hui, Wei, Jia-Yi, Li, Yuan, Zhao, Wei-Dong, Chen, Yu-Hua. 2023. Endothelial depletion of Atg7 triggers astrocyte-microvascular disassociation at blood-brain barrier. In The Journal of cell biology, 222, . doi:10.1083/jcb.202103098. https://pubmed.ncbi.nlm.nih.gov/36995368/
6. Collier, Jack J, Suomi, Fumi, Oláhová, Monika, McWilliams, Thomas G, Taylor, Robert W. 2021. Emerging roles of ATG7 in human health and disease. In EMBO molecular medicine, 13, e14824. doi:10.15252/emmm.202114824. https://pubmed.ncbi.nlm.nih.gov/34725936/
7. Xiong, Jianhua. 2015. Atg7 in development and disease: panacea or Pandora's Box? In Protein & cell, 6, 722-34. doi:10.1007/s13238-015-0195-8. https://pubmed.ncbi.nlm.nih.gov/26404030/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen