C57BL/6JCya-Dgkgem1/Cya
Common Name:
Dgkg-KO
Product ID:
S-KO-18550
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Dgkg-KO
Strain ID
KOCMP-110197-Dgkg-B6J-VA
Gene Name
Product ID
S-KO-18550
Gene Alias
2900055E17Rik; 90kDa; Dagk3; E430001K23Rik; mKIAA4131
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dgkgem1/Cya mice (Catalog S-KO-18550) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000089925
NCBI RefSeq
NM_138650
Target Region
Exon 4~5
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Dgkg, encoding diacylglycerol kinase gamma, is a key enzyme in glycerophospholipid metabolism and phosphatidylinositol signaling pathways [7]. Diacylglycerol kinases are important regulators of cell signaling, and Dgkg may be involved in various biological processes [4].
In hepatocellular carcinoma (HCC), endothelial Dgkg promoted tumor angiogenesis and immunosuppressive regulatory T-cell differentiation. Hypoxia-induced HIF-1α activates Dgkg transcription, and upregulated Dgkg promotes HCC progression via the ZEB2/TGF-β1 axis. Targeting endothelial Dgkg enhanced the efficiency of dual blockade of PD-1 and VEGFR-2 in a mouse HCC model [1].
In Wagyu cattle, a homozygous missense variant in Dgkg was associated with hepatic fibrinogen storage disease [2].
In an epileptic patient, a heterozygous mutation in Dgkg was found, and an induced pluripotent stem cell line was generated for studying epilepsy mechanisms [3].
In colorectal cancer, Dgkg was hypermethylated, suppressing its expression. Ectopic expression of wild-type and mutant Dgkg suppressed cell migration, invasion, and Rac1 activity, suggesting a tumor-suppressor role [4].
In acute myeloid leukemia, high Dgkg expression was associated with a more favorable prognosis according to the TGCA database [5].
In cortical GABAergic interneurons, Dgkg was dominantly expressed in somatostatin-expressing neurons, and its functional study indicated an alteration in neurite outgrowth [6].
In glioblastoma, a hypoxia-induced alternative splicing transcript Dgkg-Δ exon13 promoted cellular proliferation, migration, and invasion [8].
In conclusion, Dgkg plays diverse roles in multiple biological processes and diseases. Its functions in tumor angiogenesis, immune evasion, and cell migration/invasion in cancers, as well as in neurological and metabolic-related diseases, have been revealed through various research models, including in vivo studies in mouse models. Understanding Dgkg provides insights into disease mechanisms and potential therapeutic targets.
References：
1. Zhang, Liren, Xu, Jiali, Zhou, Suiqing, Li, Qing, Wang, Xuehao. 2023. Endothelial DGKG promotes tumor angiogenesis and immune evasion in hepatocellular carcinoma. In Journal of hepatology, 80, 82-98. doi:10.1016/j.jhep.2023.10.006. https://pubmed.ncbi.nlm.nih.gov/37838036/
2. Jacinto, Joana G P, Wohlsein, Peter, Häfliger, Irene M, Grünberg, Walter, Drögemüller, Cord. 2023. A missense variant in DGKG as a recessive functional variant for hepatic fibrinogen storage disease in Wagyu cattle. In Journal of veterinary internal medicine, 37, 2631-2637. doi:10.1111/jvim.16865. https://pubmed.ncbi.nlm.nih.gov/37681469/
3. Liu, Ya-Qing, Ling, Tiao-Wen, Wang, Hong-Yan, Song, Wen-Jun, Wang, Tian-Cheng. 2022. Generation of an integration-free induced pluripotent stem cell line (LZUSHI001-A) from an epileptic patient with DGKG mutation. In Stem cell research, 61, 102768. doi:10.1016/j.scr.2022.102768. https://pubmed.ncbi.nlm.nih.gov/35421845/
4. Kai, Masahiro, Yamamoto, Eiichiro, Sato, Akiko, Toyota, Minoru, Suzuki, Hiromu. 2017. Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer. In Molecular carcinogenesis, 56, 1743-1752. doi:10.1002/mc.22631. https://pubmed.ncbi.nlm.nih.gov/28218473/
5. Gravina, Teresa, Boggio, Chiara Maria Teresa, Gorla, Elisa, Corà, Davide, Baldanzi, Gianluca. 2023. Role of Diacylglycerol Kinases in Acute Myeloid Leukemia. In Biomedicines, 11, . doi:10.3390/biomedicines11071877. https://pubmed.ncbi.nlm.nih.gov/37509516/
6. Fukumoto, Keita, Tamada, Kota, Toya, Tsuyoshi, Yanagawa, Yuchio, Takumi, Toru. 2017. Identification of genes regulating GABAergic interneuron maturation. In Neuroscience research, 134, 18-29. doi:10.1016/j.neures.2017.11.010. https://pubmed.ncbi.nlm.nih.gov/29203264/
7. Taniguchi-Ponciano, Keiko, Hinojosa-Alvarez, Silvia, Hernandez-Perez, Jesus, Marrero-Rodriguez, Daniel, Mercado, Moises. 2024. Longitudinal multiomics analysis of aggressive pituitary neuroendocrine tumors: comparing primary and recurrent tumors from the same patient, reveals genomic stability and heterogeneous transcriptomic profiles with alterations in metabolic pathways. In Acta neuropathologica communications, 12, 142. doi:10.1186/s40478-024-01796-x. https://pubmed.ncbi.nlm.nih.gov/39217365/
8. Yang, Ming, Chu, Liangzhao, Lin, Shukai, Han, Feng, Liu, Jian. 2025. The alternatively spliced diacylglycerol kinase gamma-Δ exon13 transcript generated under hypoxia promotes glioblastoma progression. In Oncology research, 33, 1189-1198. doi:10.32604/or.2024.055102. https://pubmed.ncbi.nlm.nih.gov/40296900/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen