C57BL/6JCya-Blvraem1/Cya
Common Name:
Blvra-KO
Product ID:
S-KO-18581
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Blvra-KO
Strain ID
KOCMP-109778-Blvra-B6J-VB
Gene Name
Product ID
S-KO-18581
Gene Alias
0610006A11Rik; 2500001N03Rik; Blvr
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Blvraem1/Cya mice (Catalog S-KO-18581) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000002064
NCBI RefSeq
NM_026678
Target Region
Exon 4~5
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Blvra, short for biliverdin reductase A, is a pleiotropic enzyme. It converts biliverdin-IX-alpha into bilirubin-IX-alpha, an antioxidant and anti-nitrosative compound. This conversion is part of the heme degradation pathway. Bilirubin has various physiological functions such as antioxidant activity, immunosuppressive effects, and is recognized as a hormone with endocrine actions affecting cellular signaling pathways [1].
In Parkinson's disease (PD) research, a GK2-BLVRA fusion protein protected dopaminergic neuronal cells from oxidative stress in vitro and in vivo in a PD animal model. It reduced reactive oxygen species production, DNA damage, suppressed MAPK activation, and modulated apoptosis-related protein expression [2]. In hepatocellular carcinoma (HCC) and colorectal cancer (CRC), knockdown of Blvra in cell lines suppressed cell proliferation, invasion, and migration, and induced apoptosis. It was also found that Blvra exerted its effects via the Wnt/β-catenin pathway in both types of cancer [3,4]. In neonatal hyperbilirubinaemia, the G-allele at the rs699512 locus of the Blvra gene was associated with a decreased risk, suggesting that Blvra genetic variants are potential markers for predicting this disorder [5].
In conclusion, Blvra plays a crucial role in multiple biological processes, especially in antioxidant defense and cellular redox regulation. Its involvement in diseases like PD, HCC, CRC, and neonatal hyperbilirubinaemia has been revealed through functional studies. These findings from in vivo and in vitro models help understand the underlying mechanisms of these diseases and may provide new therapeutic targets.
References:
1. Vitek, Libor, Hinds, Terry D, Stec, David E, Tiribelli, Claudio. 2023. The physiology of bilirubin: health and disease equilibrium. In Trends in molecular medicine, 29, 315-328. doi:10.1016/j.molmed.2023.01.007. https://pubmed.ncbi.nlm.nih.gov/36828710/
2. Choi, Yeon Joo, Kwon, Hyun Jung, Shin, Min Jea, Eum, Won Sik, Choi, Soo Young. 2023. Protective effect of GK2 fused BLVRA protein against oxidative stress-induced dopaminergic neuronal cell damage. In The FEBS journal, 290, 2923-2938. doi:10.1111/febs.16721. https://pubmed.ncbi.nlm.nih.gov/36688733/
3. Chen, Xinju, Yang, Fangming, Zhang, Chuanlei, Chen, Xiaoqi, Zhao, Wenxia. 2024. BLVRA exerts its biological effects to induce malignant properties of hepatocellular carcinoma cells via Wnt/β-catenin pathway. In Journal of molecular histology, 55, 159-167. doi:10.1007/s10735-023-10179-w. https://pubmed.ncbi.nlm.nih.gov/38216836/
4. Mao, Haiyan, Xu, Yuan, Zhang, Zhengrong, Liu, Siping, Bo, Ping. 2020. Biliverdin Reductase A (BLVRA) Promotes Colorectal Cancer Cell Progression by Activating the Wnt/β-Catenin Signaling Pathway. In Cancer management and research, 12, 2697-2709. doi:10.2147/CMAR.S242531. https://pubmed.ncbi.nlm.nih.gov/32425592/
5. Li, Yongpei, Wu, Ting, Chen, Ling, Zhu, Yunxia. 2019. Associations between G6PD, OATP1B1 and BLVRA variants and susceptibility to neonatal hyperbilirubinaemia in a Chinese Han population. In Journal of paediatrics and child health, 55, 1077-1083. doi:10.1111/jpc.14346. https://pubmed.ncbi.nlm.nih.gov/30636082/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen