C57BL/6JCya-Chrnb2em1/Cya
Common Name:
Chrnb2-KO
Product ID:
S-KO-18684
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Chrnb2-KO
Strain ID
KOCMP-11444-Chrnb2-B6J-VA
Gene Name
Product ID
S-KO-18684
Gene Alias
Acrb-2; Acrb2; C030030P04Rik; [b]2-nAchR
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Chrnb2em1/Cya mice (Catalog S-KO-18684) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029562
NCBI RefSeq
NM_009602
Target Region
Exon 2~5
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
CHRNB2, encoding the β2 subunit of the α4β2 nicotine acetylcholine receptor, is involved in controlling neurotransmitter release. It plays a role in various physiological and pathologic processes, such as being associated with pathways like the β -catenin pathway, PI3K -AKT, and JAK -STAT pathways [1,3]. Genetic models, especially gene knockout models, can be valuable for studying its function.
In pancreatic cancer, knockdown of CHRNB2 expression acetylcholine-independently increases cell migration and invasion, while overexpression decreases these abilities. CHRNB2 negatively regulates the β -catenin pathway, EMT, migration, and invasion of pancreatic cancer cells [1].
In gastric cancer, knockdown of CHRNB2 attenuates cell proliferation, and knockout significantly influences cell survival and metastasis-related functions. Blockade of CHRNB2 signaling with a monoclonal antibody shows promise for controlling metastasis [3].
In mice, exposure to nicotine vapor reduces CHRNB2 expression, and in humans, rare coding variants in CHRNB2 reduce the likelihood of heavy smoking [2,5]. Also, variants in CHRNB2 are found in patients with epilepsy, including insular epilepsy and autosomal dominant nocturnal frontal lobe epilepsy, and in a Chinese boy with refractory seizures and developmental delay [4,6,7].
In conclusion, CHRNB2 is crucial in regulating cell migration, invasion, and proliferation in cancer, and in influencing smoking behavior and epilepsy-related phenotypes. Gene knockout and other functional studies in model organisms and humans have provided insights into its role in these disease areas, helping to understand the underlying molecular mechanisms and potentially guiding the development of new therapeutic strategies.
References:
1. Qin, Cheng, Li, Tianhao, Wang, Yuanyang, Zhao, Yutong, Wang, Weibin. 2022. CHRNB2 represses pancreatic cancer migration and invasion via inhibiting β-catenin pathway. In Cancer cell international, 22, 340. doi:10.1186/s12935-022-02768-8. https://pubmed.ncbi.nlm.nih.gov/36344976/
2. Rajagopal, Veera M, Watanabe, Kyoko, Mbatchou, Joelle, Baras, Aris, Coppola, Giovanni. 2023. Rare coding variants in CHRNB2 reduce the likelihood of smoking. In Nature genetics, 55, 1138-1148. doi:10.1038/s41588-023-01417-8. https://pubmed.ncbi.nlm.nih.gov/37308787/
3. Kanda, Mitsuro, Shimizu, Dai, Nakamura, Shunsuke, Katsuno, Masahisa, Kodera, Yasuhiro. 2021. Blockade of CHRNB2 signaling with a therapeutic monoclonal antibody attenuates the aggressiveness of gastric cancer cells. In Oncogene, 40, 5495-5504. doi:10.1038/s41388-021-01945-9. https://pubmed.ncbi.nlm.nih.gov/34331011/
4. Cadieux-Dion, Maxime, Meneghini, Simone, Villa, Chiara, Becchetti, Andrea, Nguyen, Dang Khoa. 2020. Variants in CHRNB2 and CHRNA4 Identified in Patients with Insular Epilepsy. In The Canadian journal of neurological sciences. Le journal canadien des sciences neurologiques, 47, 800-809. doi:10.1017/cjn.2020.126. https://pubmed.ncbi.nlm.nih.gov/32536355/
5. Giner, Priscilla, Maynez-Anchondo, Liliana, Liley, Anna E, Simon, Nicholas W, Mendez, Ian A. 2022. Increased Risky Choice and Reduced CHRNB2 Expression in Adult Male Rats Exposed to Nicotine Vapor. In International journal of molecular sciences, 23, . doi:10.3390/ijms23031231. https://pubmed.ncbi.nlm.nih.gov/35163155/
6. Wang, Sanmei, Cui, Di, Ling, Xiuxin, Hou, Yu, Sun, Jing. 2023. Two novel variants of the STXBP1 and CHRNB2 genes identified in a Chinese boy with refractory seizures and developmental delay. In Psychiatric genetics, 33, 206-212. doi:10.1097/YPG.0000000000000345. https://pubmed.ncbi.nlm.nih.gov/37706497/
7. Phillips, H A, Favre, I, Kirkpatrick, M, Bertrand, D, Mulley, J C. 2000. CHRNB2 is the second acetylcholine receptor subunit associated with autosomal dominant nocturnal frontal lobe epilepsy. In American journal of human genetics, 68, 225-31. doi:. https://pubmed.ncbi.nlm.nih.gov/11104662/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen