C57BL/6JCya-Capza1em1/Cya
Common Name
Capza1-KO
Product ID
S-KO-18742
Backgroud
C57BL/6JCya
Strain ID
KOCMP-12340-Capza1-B6J-VB
When using this mouse strain in a publication, please cite “Capza1-KO Mouse (Catalog S-KO-18742) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Capza1-KO
Strain ID
KOCMP-12340-Capza1-B6J-VB
Gene Name
Product ID
S-KO-18742
Gene Alias
CAPZ, CAZ1, Cappa1, capZ alpha-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 3
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000094028
NCBI RefSeq
NM_009797
Target Region
Exon 2
Size of Effective Region
~1.2 kb
Overview of Gene Research
Capza1, also known as capping actin protein of muscle Z-line alpha subunit 1, is a subunit of the capping protein complex CapZ, an actin-binding complex that can regulate actin cytoskeleton remodelling [3,6]. Actin filament assembly, which Capza1 is involved in, plays a crucial role in maintaining tumour cell morphology and movement, and thus is important in biological processes like epithelial-mesenchymal transition (EMT) [3].
In various cancers, Capza1 shows diverse functions. In lung adenocarcinoma (LUAD), high Capza1 expression has an unfavourable prognostic value, and it is associated with immune infiltration cells, immune checkpoints etc., suggesting it as a potential immunological antitumor target [1]. In gastric cancer, overexpression of Capza1 is associated with well-differentiated histology, smaller tumour size etc., and in vitro, forced expression of Capza1 decreases gastric cancer cell migration and invasion [7]. In hepatocellular carcinoma (HCC), Capza1 inhibits EMT by regulating actin cytoskeleton remodelling, reducing cell metastatic ability, and low expression of Capza1 promotes HCC cell invasion and migration both in vitro and in vivo [3,9]. Also, Helicobacter pylori-infected CAPZA1-overexpressing cells can give rise to cancer stem-cell marker CD44v9-positive cells, and CAPZA1 can inhibit autolysosome formation in H. pylori-infected gastric epithelial cells, increasing the risk of gastric carcinogenesis [2,4]. Moreover, polymorphisms in CAPZA1 may be associated with the development of gastric mucosal atrophy [5]. In addition, FAM21C can promote HCC invasion and metastasis by inhibiting the capping ability of CAPZA1 [6], and exosome-delivered circSTAU2 can inhibit gastric cancer progression by targeting the miR-589/CAPZA1 axis [8].
In conclusion, Capza1 plays a significant role in cancer-related biological processes, especially in tumour cell migration, invasion, and EMT, mainly through its function in regulating actin cytoskeleton remodelling. Studies on Capza1 in different cancer models help understand its role in cancer development, providing potential targets for cancer prognosis and treatment.
References：
1. Qin, Tingting, Xiang, Wanping, Mao, Yiming, Yang, Zhihao, Zhang, Hongpan. 2023. NcRNA-regulated CAPZA1 associated with prognostic and immunological effects across lung adenocarcinoma. In Frontiers in oncology, 12, 1025192. doi:10.3389/fonc.2022.1025192. https://pubmed.ncbi.nlm.nih.gov/36686785/
2. Tsugawa, Hitoshi, Mori, Hideki, Matsuzaki, Juntaro, Suematsu, Makoto, Suzuki, Hidekazu. 2018. CAPZA1 determines the risk of gastric carcinogenesis by inhibiting Helicobacter pylori CagA-degraded autophagy. In Autophagy, 15, 242-258. doi:10.1080/15548627.2018.1515530. https://pubmed.ncbi.nlm.nih.gov/30176157/
3. Huang, Deng, Cao, Li, Zheng, Shuguo. 2017. CAPZA1 modulates EMT by regulating actin cytoskeleton remodelling in hepatocellular carcinoma. In Journal of experimental & clinical cancer research : CR, 36, 13. doi:10.1186/s13046-016-0474-0. https://pubmed.ncbi.nlm.nih.gov/28093067/
4. Tsugawa, Hitoshi, Kato, Chihiro, Mori, Hideki, Suematsu, Makoto, Suzuki, Hidekazu. 2019. Cancer Stem-Cell Marker CD44v9-Positive Cells Arise From Helicobacter pylori-Infected CAPZA1-Overexpressing Cells. In Cellular and molecular gastroenterology and hepatology, 8, 319-334. doi:10.1016/j.jcmgh.2019.05.008. https://pubmed.ncbi.nlm.nih.gov/31146068/
5. Yamaguchi, Naoyuki, Sakaguchi, Takuki, Isomoto, Hajime, Yashima, Kazuo, Tsukamoto, Kazuhiro. 2023. Polymorphism in autophagy-related genes LRP1 and CAPZA1 may promote gastric mucosal atrophy. In Genes and environment : the official journal of the Japanese Environmental Mutagen Society, 45, 18. doi:10.1186/s41021-023-00274-5. https://pubmed.ncbi.nlm.nih.gov/37198664/
6. Lu, Yao, Huang, Deng, Wang, Baolin, Song, Juxian, Zheng, Shuguo. 2022. FAM21C Promotes Hepatocellular Carcinoma Invasion and Metastasis by Driving Actin Cytoskeleton Remodeling via Inhibiting Capping Ability of CAPZA1. In Frontiers in oncology, 11, 809195. doi:10.3389/fonc.2021.809195. https://pubmed.ncbi.nlm.nih.gov/35096613/
7. Lee, Young-Joon, Jeong, Sang-Ho, Hong, Soon-Chan, Yoo, Jiyun, Ko, Gyung Hyuck. 2013. Prognostic value of CAPZA1 overexpression in gastric cancer. In International journal of oncology, 42, 1569-77. doi:10.3892/ijo.2013.1867. https://pubmed.ncbi.nlm.nih.gov/23545944/
8. Zhang, Chenggang, Wei, Guanxin, Zhu, Xiuxian, Wu, Chuanqing, Tao, Kaixiong. 2023. Exosome-Delivered circSTAU2 Inhibits the Progression of Gastric Cancer by Targeting the miR-589/CAPZA1 Axis. In International journal of nanomedicine, 18, 127-142. doi:10.2147/IJN.S391872. https://pubmed.ncbi.nlm.nih.gov/36643863/
9. Huang, Deng, Cao, Li, Xiao, Le, Zheng, Ping, Zheng, Shu-Guo. 2019. Hypoxia induces actin cytoskeleton remodeling by regulating the binding of CAPZA1 to F-actin via PIP2 to drive EMT in hepatocellular carcinoma. In Cancer letters, 448, 117-127. doi:10.1016/j.canlet.2019.01.042. https://pubmed.ncbi.nlm.nih.gov/30742939/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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