C57BL/6JCya-Zfp703em1/Cya
Common Name:
Zfp703-KO
Product ID:
S-KO-18765
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Zfp703-KO
Strain ID
KOCMP-353310-Zfp703-B6J-VB
Gene Name
Product ID
S-KO-18765
Gene Alias
1110032O19Rik; Csmn1; End2; Zeppo1; Znf703; Zpo1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfp703em1/Cya mice (Catalog S-KO-18765) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000154256
NCBI RefSeq
NM_001101502
Target Region
Exon 2
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Zfp703, a gene with no common aliases provided, is involved in several important biological processes. It functions as a feedback suppressor of the Wnt/β -catenin signaling pathway, which controls embryonic development and adult stem cell maintenance [1]. Malregulation of this pathway can lead to embryonic malformations and cancer, highlighting Zfp703's significance in maintaining proper cellular function. Genetic models could be valuable for further studying its functions.
In embryonic stem cells, Zfp703 was shown to inhibit Wnt/β -catenin activity in reporter assays and Wnt -dependent mesoderm differentiation. It binds directly to Tcf1, inhibiting β -catenin/Tcf1 complex formation independently of the Groucho/Tle transcriptional corepressor [1]. Additionally, retinoic acid (RA) treatment of embryonic stem cells differentiated to neuromesodermal progenitors identified Zfp703 as an early RA signaling target, suggesting a role in controlling differentiation of these progenitors to neural and mesodermal lineages [2]. In the prostate gland, androgen deprivation led to transient expression of Zfp703, indicating its involvement in the gland's adaptation to a hypoandrogenic environment [3].
In conclusion, Zfp703 plays essential roles in regulating the Wnt/β -catenin signaling pathway, in the response to retinoic acid during differentiation of neuromesodermal progenitors, and in the prostate gland's adaptation to androgen deprivation. Studies using genetic models, though not specifically KO/CKO mouse models in the provided references, could potentially further elucidate its functions in these and related disease-relevant processes such as embryonic malformations, cancer, and prostatic diseases.
References:
1. Kumar, Amit, Chalamalasetty, Ravindra B, Kennedy, Mark W, Garriock, Robert J, Yamaguchi, Terry P. 2016. Zfp703 Is a Wnt/β-Catenin Feedback Suppressor Targeting the β-Catenin/Tcf1 Complex. In Molecular and cellular biology, 36, 1793-802. doi:10.1128/MCB.01010-15. https://pubmed.ncbi.nlm.nih.gov/27090637/
2. Cunningham, Thomas J, Colas, Alexandre, Duester, Gregg. 2016. Early molecular events during retinoic acid induced differentiation of neuromesodermal progenitors. In Biology open, 5, 1821-1833. doi:10.1242/bio.020891. https://pubmed.ncbi.nlm.nih.gov/27793834/
3. Nishan, Umar, Rosa-Ribeiro, Rafaela, Cesar, Carlos Lenz, Carvalho, Hernandes F. 2019. Transcription regulators are transiently expressed during the prostate gland adaptation to the hypoandrogenic environment. In Histology and histopathology, 34, 1025-1036. doi:10.14670/HH-18-105. https://pubmed.ncbi.nlm.nih.gov/30912572/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen