C57BL/6JCya-Lipfem1/Cya
Common Name:
Lipf-KO
Product ID:
S-KO-18846
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Lipf-KO
Strain ID
KOCMP-67717-Lipf-B6J-VB
Gene Name
Product ID
S-KO-18846
Gene Alias
2310051B21Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lipfem1/Cya mice (Catalog S-KO-18846) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025680
NCBI RefSeq
NM_026334
Target Region
Exon 4
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
LipF, also known as gastric lipase, is involved in lipid-metabolic functions and various lipid metabolism processes [2].
In Mycobacterium tuberculosis, reduced LipF expression may contribute to the development of rifampicin (RIF) and streptomycin (STR) resistance. Expressing lipF from Mycobacterium tuberculosis in Mycobacterium smegmatis reduced the minimum inhibitory concentration of RIF and STR, and decreased the growth of M. smegmatis in the presence of these drugs, indicating that LipF expression is associated with increased sensitivity to RIF and STR in mycobacteria [1].
In gastric adenocarcinoma, the expression of LIPF is decreased, and this decrease is correlated with DGKA. The down-regulation of LIPF-DGKA may serve as potential biomarkers for the diagnosis of gastric cancer, and their decreased expression is associated with local invasion, disease stage, and poor prognosis [2].
In conclusion, LipF plays a role in mycobacterial drug resistance and gastric cancer. The study of LipF knockout or knockdown models in these contexts could potentially provide more insights into the underlying mechanisms of drug resistance in tuberculosis and the progression of gastric cancer, offering new perspectives for treatment strategies in these disease areas.
References：
1. Arriaga-Guerrero, Ana Leticia, Hernández-Luna, Carlos E, Rigal-Leal, Joyce, Bermúdez de León, Mario, Peñuelas-Urquides, Katia. 2020. LipF increases rifampicin and streptomycin sensitivity in a Mycobacterium tuberculosis surrogate. In BMC microbiology, 20, 132. doi:10.1186/s12866-020-01802-x. https://pubmed.ncbi.nlm.nih.gov/32450809/
2. Kong, Yi, Zheng, Yan, Jia, Yanfei, Li, Ping, Wang, Yunshan. 2016. Decreased LIPF expression is correlated with DGKA and predicts poor outcome of gastric cancer. In Oncology reports, 36, 1852-60. doi:10.3892/or.2016.4989. https://pubmed.ncbi.nlm.nih.gov/27498782/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen