C57BL/6JCya-Arhgdibem1/Cya
Common Name
Arhgdib-KO
Product ID
S-KO-18912
Backgroud
C57BL/6JCya
Strain ID
KOCMP-11857-Arhgdib-B6J-VB
When using this mouse strain in a publication, please cite “Arhgdib-KO Mouse (Catalog S-KO-18912) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Arhgdib-KO
Strain ID
KOCMP-11857-Arhgdib-B6J-VB
Gene Name
Product ID
S-KO-18912
Gene Alias
D4, Gdid4, Ly-GDI
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000032344
NCBI RefSeq
NM_007486
Target Region
Exon 2~3
Size of Effective Region
~2.1 kb
Overview of Gene Research
Arhgdib, also known as Rho guanosine diphosphate dissociation inhibitor 2 (RhoGDI2), is a negative regulator of the Rho guanosine triphosphate (RhoGTP)ases RhoA, Rac1, and Cdc42 [4]. It mainly functions in regulating the actin network in various cells and is involved in multiple biological processes [4].
In bladder cancer, KDM6A promotes the transcription of Arhgdib by demethylating histone H3 lysine di/trimethylation (H3K27me2/3), which in turn inhibits Rac1 and blocks metastasis [1]. In breast cancer, Arhgdib expression is significantly higher in cancer tissues compared to benign tissues, and its depletion decreases cancer cell proliferation, migration, and invasion [5]. In kidney transplantation, pre-transplant anti-Arhgdib antibodies are associated with long-term kidney graft loss, and Arhgdib gene expression in the kidney is related to allograft outcome [2,3]. In trophoblasts, PD-L1 enhances migration and invasion by upregulating Arhgdib via transcription factor PU.1 [6]. In bladder cancer, ATG7-mediated autophagy promotes Arhgdib mRNA stability, leading to cancer cell invasion [7].
In conclusion, Arhgdib plays a crucial role in multiple biological processes and diseases, especially in cancer metastasis and kidney transplantation outcomes. The research on Arhgdib using various models helps to understand its functions in different physiological and pathological conditions, providing potential targets for disease treatment.
References：
1. Liu, Lei, Cui, Jianfeng, Zhao, Yajing, Shi, Benkang, Zou, Yongxin. 2021. KDM6A-ARHGDIB axis blocks metastasis of bladder cancer by inhibiting Rac1. In Molecular cancer, 20, 77. doi:10.1186/s12943-021-01369-9. https://pubmed.ncbi.nlm.nih.gov/34006303/
2. Kamburova, Elena G, Gruijters, Maartje L, Kardol-Hoefnagel, Tineke, Bemelman, Frederike J, Otten, Henny G. 2019. Antibodies against ARHGDIB are associated with long-term kidney graft loss. In American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 19, 3335-3344. doi:10.1111/ajt.15493. https://pubmed.ncbi.nlm.nih.gov/31194283/
3. Senev, Aleksandar, Otten, Henny G, Kamburova, Elena G, Emonds, Marie-Paule, Naesens, Maarten. . Antibodies Against ARHGDIB and ARHGDIB Gene Expression Associate With Kidney Allograft Outcome. In Transplantation, 104, 1462-1471. doi:10.1097/TP.0000000000003005. https://pubmed.ncbi.nlm.nih.gov/31651716/
4. Kardol-Hoefnagel, Tineke, van Logtestijn, Sofie A L M, Otten, Henny G. 2020. A Review on the Function and Regulation of ARHGDIB/RhoGDI2 Expression Including the Hypothetical Role of ARHGDIB/RhoGDI2 Autoantibodies in Kidney Transplantation. In Transplantation direct, 6, e548. doi:10.1097/TXD.0000000000000993. https://pubmed.ncbi.nlm.nih.gov/32548242/
5. Wang, Xiaonan, Bi, Xiaomin, Huang, Xing, Guo, Qianying, Wu, Zhengsheng. . Systematic investigation of biomarker-like role of ARHGDIB in breast cancer. In Cancer biomarkers : section A of Disease markers, 28, 101-110. doi:10.3233/CBM-190562. https://pubmed.ncbi.nlm.nih.gov/32176626/
6. Zhang, Ruonan, Jia, Linyan, Meng, Lulu, Duan, Tao, Wang, Kai. 2022. PD-L1 enhances migration and invasion of trophoblasts by upregulating ARHGDIB via transcription factor PU.1. In Cell death discovery, 8, 395. doi:10.1038/s41420-022-01171-6. https://pubmed.ncbi.nlm.nih.gov/36138021/
7. Zhu, Junlan, Tian, Zhongxian, Li, Yang, Huang, Haishan, Huang, Chuanshu. 2019. ATG7 Promotes Bladder Cancer Invasion via Autophagy-Mediated Increased ARHGDIB mRNA Stability. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 6, 1801927. doi:10.1002/advs.201801927. https://pubmed.ncbi.nlm.nih.gov/31016112/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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